We narrowed to 658 results for: CHI;

...tissue, these protein barcodes allow for efficient machine-learning segmentation of neurons and proofreading...been used in expansion microscopy workflows to achieve high-resolution optical imaging down to individual... microscopy and reconstruction of neurons with machine learning. Adapted from Park & Sheridan et al. (... Park, S.Y., Sheridan, A., An, B., Jarvis, E., Lyudchik, J., Patton, W., Axup, J.Y., Chan, S.W., Damstra...

...cells in an organism and use the host cellular machinery to produce more virions. B) Viral plasmids have...to packaging cell lines and use their cellular machinery to generate viral vectors for research and gene...viral genome contains only the necessary genes to achieve this goal. When a virus recognizes a host cell,...genome into the cytoplasm and hijacks the host’s machinery to produce more viruses. In this way, viruses ...

...zebrafish research. China Zebrafish Resource Center (CZRC) - A resource center based in China focused on collecting...Sean Megason Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish...lineages and cell types in the vertebrate brain - Alex Schier Lab Other Tools of Interest A few examples of popular...

...Parkinson's Lynda Chin 21691 pLenti6/V5/DEST-VPS35 VPS35 V5 CMV Parkinson's Lynda Chin 21827 TLS 1: hTLS.pBSKS...Gabriele Kaminski Schierle 108866 pT7-7 aSyn C141 SNCA T7 Parkinson's Gabriele Kaminski Schierle 108867 pET29b...Kaminski Schierle 108868 pLJM1 EGFP-Tau MAPT GFP CMV Parkinson's, FTD Gabriele Kaminski Schierle 109321 ... mScarlet CMV ALS Kyoko Chiba 184826 pSN838 KIF5A mScarlet CMV ALS Kyoko Chiba 184827 pSN526 KIF5A mScarlet...Strep polH ALS Kyoko Chiba 184832 pSN840 KIF5A mScarlet Strep polH ALS Kyoko Chiba 184894 pMRX-IZ-ANXA11...-2b] - Chimeric FXN CMV Friedreich ataxia James Trimmer 227032 Anti-Pink1 [N4/15-2b] - Chimeric PINK1 ...pMXs-mPKR-FLAG-IG EIF2AK2 Flag LTR VWM disease Seiichi Oyadomari 101848 pTO_CHMP2B_LAP CHMP2B LAP CMV ...

...simplicity, other promoter elements and transcription machinery are not shown. Tetracycline Off (Tet-Off) The ...doxycycline (Zhou et al., 2006). Tet-On 3G systems can achieve >10,000-fold induction of the gene of interest....cell lines or transgenic animals can be made to achieve greater control over expression with flexibility...Galla, M., Maetzig, T., Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral and transposon-based ...

... or 20 matching nucleotides, the second binds to mcherry with either 0, 11, 18, or 20 matching nucleotides...possible to study the effect of the amount and stoichiometry of enzymes. The pLC97 plasmid allows for quick...

...Fab fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments...antibodies as Fab' fragments, isotype-switched chimeric antibodies, and Fc-silent mutants. Functional ...

...-Puro 73039 Expresses Nluc and copGFP from the chicken beta-actin CMV enhancer pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro... the EF1 promoter while pICPIS-CB contains the Chicken-beta actin promoter with CMV enhancer. pAdx-CMV-LacZ...polyA pICPIS-CB 73356 AdenoX shuttle vector with chicken-beta actin CMV enhancer promoter SV40 polyA Addgene...

...ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method. Titering by the...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...

...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

...gently touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well...nothing falls into the plate. Leave dilutions on the chilled 96-well freezer block in the BSC until ready to...Prepare the Master Mix Place a ddPCR plate onto a chilled 96-well freezer block and set aside in the droplet...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 20 µL of the master ...aspirating the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. In this protocol...

...2015). A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation...2012). Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon ...Söll, D. (2011). Expanding the Genetic Code of Escherichia coli with Phosphoserine . Science , 333 (6046...

...sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular tool for scientists... of Renilla luciferase Reuben Shaw 48688 pUltra-Chili-Luc Firefly Ubc Lentiviral expression of firefly... Mammalian expression of firefly luciferase Ming-Chih Lai 100984 pGL4.18 CMV-Luc Firefly CMV Mammalian...

...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to ...Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent...ice for 15-20 minutes or until completely thawed. Chill a box of 200 µL micropipette tips in a -20 °C freezer...kV, 1 pulse. Aliquot 25 µL DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation involves...sterile LB at 4 ℃. Day 2 (morning) Before beginning, prechill at least four conical tubes on ice and ensure ...

... order primers that are 60 bp long, with 30 bp matching the end of the adjacent fragment and 30 bp annealing...the assembled parts. Tips and Troubleshooting “Stitching” fragments together using oligos When you need...Pro-Tip Please note that the way to design the “stitching” primers and the amounts of primers to include...TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA...Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...

... gently touching the screen. Preparation of the Master Mix Place a ddPCR plate onto a chilled 96-well ...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 16 µL of the master ...pipetting the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the...

...Resources iGEM - International Genetically Engineered Machine Foundation Registry of Standard Biological Parts...papers on Syn Bio SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource Synthetic...

...Gi) - Inhibition HA fusion none 2, 5 Christopher Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D(Gq) - Activation...Activation HA fusion none 2, 5, rg* Christopher Richie 50467 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi...

...Infrastructure Kit Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains...(miRNAs) or small interfering RNAs (siRNAs) to achieve gene silencing in plants. It also includes plasmids...

...Schroeder, S., Krüger, N., Herrler, T., Erichsen, S., Schiergens, T. S., Herrler, G., Wu, N. H., Nitsche, A., ...Kogiso, H., Raveau, M., Yamakawa, K., Saito, D., Ikeuchi, Y., Okazaki, T., Asano, S., Inui, T., Marunaka...

...Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible recognition sites flank... for Site-specific Recombination in Drosophila Teaching an Old DOG New Tricks: Controlling Protein Activity...

...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below the primer...liquid to the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from...

...minutes at 95°C in a PCR machine or in a beaker of boiling water. If using a PCR machine, incubate the sample... that does not contain antibiotics. h. Without touching the sides of the dish, gently add DNA:FuGENE® ...PubMed . Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Zufferey R et...

... Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...

...SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down to location, location...proceeding, we recommend asking labmates/colleagues, searching the literature, or discussing with your PI to ...

...Tommaso Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Tommaso Patriarchi Oxytocin...

...image and the resolution your microscope can achieve. You achieve the highest resolution when your image is...

...surface and should be handled carefully. Avoid touching the cells when replacing media. T-175 flask, Corning...plasmids, we can determine the total μg/bp we need to achieve a 1:1:1 molar ratio of each plasmid: 2000 μg / ...

...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013...

...assemble TAL effector repeat arrays on a single architectural framework and the DNAs produced by all three...