We narrowed to 154 results for: 11
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TypeBlog Post... Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, pp...Toolbox for Plants.” ACS Synthetic Biology, vol. 3, no. 11, American Chemical Society (ACS), 20 Feb. 2014, pp...Mammalian Cells.” ACS Synthetic Biology, vol. 8, no. 11, American Chemical Society (ACS), 5 Nov. 2019, pp...
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mTOR Pathway
TypeCollection...LKB1 Also known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin... -
AAVED
TypeCollection...Retrograde Tracing Gowan Tervo and Sarada Viswanathan 11:00 AM Circuit Tracing Ali Cetin 12:00 PM Lunch 1:... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post...not include stem loop 3, and guide RNA nucleotides 11-20 are not resolved due to structural disorder. HNH...DNA target are essential for cleavage. Nucleotides 11-20 are disordered and don’t appear in the Figure ... editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739... -
AAV Titration by qPCR Using SYBR Green Technology
TypeProtocol... = 1.59 x 10 11 molecules/μL To obtain a solution at 2 x 10 9 molecules/μL: 1.59 x 10 11 / 2 x 10 9 = ... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...from researchers at Amgen, it was stated that only 11% of the academic research in the literature was reproducible...specific compliance requirements – e.g. 21 CFR part 11, HIPAA? Answering this question helps you evaluate... -
CRISPR Activation: A Practical Guide
TypeBlog Post...Derived Transcriptional Activator Systems. PLoS ONE 11:e0158294 . https://doi.org/10.1371/journal.pone.0158294...control of endogenous gene activation. Nat Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Tanenbaum... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post... National Academy of Sciences 110.18 (2013). 7306-11. Pubmed PMID: 23589850. 9. Cronin, J., Zhang, X.Y...PMID: 22407723. PubMed Central PMCID: PMC3456965. 11. Zhang B., Metharom P., Jullie H., Ellem K.A., Cleghorn... -
Download Science-Themed Zoom Backgrounds from Addgene
TypeBlog Post...change? Addgene has you covered. Today we’re releasing 11 bold science-themed backgrounds! Show off your favorite... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post...Method with High Throughput Capability." PLoS ONE. 3(11): e3647 (5 November 2008). ... -
Zhang Lab CRISPR Page
TypeCollection...Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013...Sanjana NE, Shalem O, Zhang F. Nat Methods . 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed . Return... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post... for multicolor imaging across scales. Nat Commun 11, 239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev...dynamics of protein-protein interactions. Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. ... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post... data, and knowledge without exclusivity. Chapter 11: The Stem Cell Bank (CReM Bank) at the Center for... -
Addgene's Top Blog Posts from 2020
TypeBlog Post... and our new cloning grade DNA service. We've had 11 blog posts on COVID-19 featuring plasmid tools from... -
Arf GTPase Family
TypeCollection...and challenges. Molecular Biology of the Cell , 30 (11), 1249–1271. PMID: 31084567 (Link opens in a new ... -
CRISPR Plasmids - Bacteria
TypeCollection...using CRISPR. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329-6. doi: 10.1016/j.bbrc.2013.08.013... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...Polstein LR & Gersbach CA, Nat Chem Biol 2015 Mar;11(3):198-200. SunTag system for single molecule imaging... Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on their ...basis for Gateway cloning. The combination of all 11 att pairs in series with unique spacer sequences ...pMemoryArray) gives a DNA sequence capable of recording 11 bits (1.375 bytes) of information. This leads to ...pMemoryArray. Yang et al., Nat Methods 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Bioluminescent... Schmidl, et al. ACS Synth Biol. 2014 Nov 21;3(11):820-31. Inncredible new tools for epitope tagging... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the cap gene which... -
Build Your CRISPR Vocabulary
TypeBlog Post... editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...with Addgene Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR... -
Important Considerations When Using AAVs
TypeBlog Post...of AAVs as a new tool for neuroanatomical tracing (11). With the ability to produce neuron specific transfection...therapy 18.2 (2007): 171-182. PubMed PMID: 17328683. 11. Wouterlood, Floris G., et al. "A fourth generation... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...agarose gel electrophoresis. Methods Mol Biol. 2009;582:11-25. doi: 10.1007/978-1-60761-340-4_2. PMID: 19763938...concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: 19263463.... -
Gibson Assembly Protocol
TypeProtocol...the mouse mitochondrial genome. Nature Methods , 7(11), 901–903. https://doi.org/10.1038/nmeth.1515 (Link... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post... the message I shared with the Addgenies on March 11, 2020 after rumors spread in our building that someone... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...unknown microbial coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 ... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post... in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene Materials...Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. 10.1038/nmeth.3047... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...Proceedings of the National Academy of Sciences USA 112(11) (2015): 3570-5. PubMed PMID: 25733849. PubMed Central...PMC4115528. Find plasmids from this paper at Addgene. 11. Cress, Brady F., et al. “CRISPathBrick: Modular ... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...photoactivation-localization microscopy (PALM, also FPALM) (11). For PALM, a small area containing inactive PA-FPs...PubMed PMID:18727145. PubMed Central PMCID:PMC4007248. 11.Hess, Samuel T., Thanu PK Girirajan, and Michael ... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...Expression CYPet-C1 - Mammalian Expression AmCyan1 453 486 11 Tetramer AmCyan1-N1 - Mammalian Expression MiCy (... 14003 Chu et al. : Nature Methods, May 2014 Vol. 11 No. 5, pp.572-8 Yu et al. : Nature Communications... -
Neurodegeneration Research Collection
TypeCollection...Bace1 plasmids . Capitini et al. iScience. 2023 Apr 11. See More Other Resources BRAIN Initiative Collection... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...combinations: biomedicine and astrobiology. During the last 11 years he has studied various aspects extreme life... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...Risk of Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https://doi.org/10.1371/journal.pone... -
K. phaffii: Rising to the Occasion
TypeBlog Post...Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389/fmicb.2020.607028 Riley... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...been critical to the Broad’s success over the last 11 years and was certainly critical to drawing me towards... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...immune cross-reactivity with SARS-CoV. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15562-9 Subbarao... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post.... Nat Methods. 2007 Mar;4(3):251-6. Epub 2007 Feb 11. PubMed. SLIC: a method for sequence- and ligation-independent... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 ... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...to create any program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the moon... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...Genome Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR...