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We narrowed to 1,029 results for: ELL

Showing: 41 - 80 of 1029 results

  1. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...purification as well as reversibly regulation of endogenous signaling activity in cells.  Let’s shed some...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based control...their use for protein purification and controlling cell signaling. Key components of OptoNBs OptoNBs have...can bind, it co-localizes with its target at the cell membrane and when the OptoNB can’t bind, it localizes... over-activated in cancer due to its involved in cell proliferation and differentiation.  Since Ras is...They expressed this OptoNB-SOS fusion protein in cells that expressed membrane-localized mCherry. With ...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade...

  3. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...Because each cell can have multiple copies of the transgene that will recombine independently, cells may acquire...expressed. Each cell remains the same color for its entire lifetime and daughter cells retain the same... the fate mapping of cell populations over time. The ability to track single cell dynamics at the organism...expressed in a single cell maintains its color over the entire length of the cell, even axons of up to ... specific cell type. The Confetti mouse was first used to describe the self-renewal of cells in the intestinal...for tracking the fate of Langerhans cells. In the Ubow mouse, cells expressing YFP, CFP, and RFP are not... of CFP++ cells permitted researchers to longitudinally map the turnover of Langerhans cells in the dermis...

  4. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...transfected cells, we count the number of tag-positive cells (tag ICC) as a percentage of total cells in the...pre-assembled in a 96-well plate. Glioma stem cells were transfected in 96-well format using an Amaxa ...Deliver csRNP into cells: Prepare cells for transfection, add csRNP and ssODN to the cell suspension, transfect...Proteins - the workhorses of the cell – never work alone in the cellular milieu. It is, therefore, critical...epitope tagging in mammalian stem cells. For easy scalability to the 96-well format, we developed a “TAG-IN...csRNP plus ssODN, is then transfected into stem cells. Cells are allowed to recover for 2-5 days and then...efficiently these components are delivered into cells; for each cell type, the transfection/delivery step needs...

  5. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ...researcher at Imperial College London, Dr. Tom Ellis. Dr. Ellis does research in synthetic biology and bioengineering...and Addgene  Many thanks to our guest, Tom Ellis! Dr. Ellis is a lead researcher at the Centre for Synthetic...yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes, and obstacles...academic researcher. Listen to the interview with Tom Ellis Podcast breakdown Intro: How Tom ended up in academia...techniques. Chapter 4: Research projects underway in the Ellis lab including yeast and E. coli engineering, microbial...specialising in engineering gene regulation in yeast. Dr. Ellis is now one of the world's leading yeast researchers...Resources at Addgene.org Find Plasmids from the Ellis Lab Find Synthetic Biology Kits Browse Our Synthetic...

  6. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...vectors (right) are transduced to target cells. Within target cells, Cas9 machinery is transiently expressed...-transformed mammalian cells. These viral vector systems also work equally well in dividing and quiescent...in order to facilitate its entry into the target cells (Beatty et al., 2012). These features make AdV an... complexes into transformed and non-transformed cells yielded rates of targeted mutagenesis similar to...nuclease-induced gene disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al., 2014... leads to a vast majority of AdV-modified human cells being subjected to scarless homology-directed genome...quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral vectors Since then...

  7. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...primary human cells  Cell therapy approaches can use base editors to correct mutations in stem cells and return... a way to improve editing in primary human cells (Gaudelli et al., 2020). One of the widely used base ...base editors with improved editing in cell lines Nicole Gaudelli, the Associate Director and Head of Gene...primary human cell editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells. ABE8 efficiently... In addition to stem cells the authors targeted 6 genes in primary human T cells using 8 selected ABE8s...to edit primary human T cells efficiently makes it a promising tool for T cell therapy development.  Reducing...base changes in cellular DNA and RNA dependent or independent of the gRNA used (Gaudelli et al., 2017)....

  8. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...Induction of Human Germ Cell Fate from Pluripotent Stem Cells. Cell Stem Cell, 2015. 17(2): p. 178-94....differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells. Cell Res, 2009. 19(...blood cells, and renal epithelial cells from urine are some of the easily isolated somatic cells that ...some of these cell types [13]:  Germ cells [14] Hepatocytes [15] Pancreatic β-cells [16] Intestinal...differentiated cell to a different cell type. While transdifferentiation is suited for switching cell types between... differentiation of human cells with synthetic modified mRNA. Cell Stem Cell, 2010. 7(5): p. 618-30. PubMed...et al., Reprogramming of T cells from human peripheral blood. Cell Stem Cell, 2010. 7(1): p. 15-9. PubMed...

  9. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ... the single-cell level can aid in drawing connections between signaling activity and cell phenotype. One...Covert, M.W. (2018). Live-cell measurements of kinase activity in single cells using translocation reporters... One method for monitoring live single-cell kinase activity is FRET, but FRET reporters are challenging...their Kinase Translocation Reporters (KTRs) whose cellular localization serves as a proxy measurement of ... used to measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between...when designing KTR experiments. Third, KTRs are not well suited for monitoring kinase activity that occurs...

  10. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...Pharmacological Sciences, a Cell Press reviews journal. Stem cells are special types of cells that can develop ...specialized cell types [2]. We general speak of 4 levels of stem cell potency: Totipotency: When a cell has ...pluripotent cells can be thought of as descendants of totipotent cells. An example of pluripotent cells is embryonic...differentiate into only one cell type. Muscle satellite cells found in the adult muscle are unipotent. They can differentiate... into myogenic precursor cells [5]. Classification of stem cells Stem cells are classified into two broad...Embryonic stem cells ESCs are pluripotent stem cells that are able to form all adult cell types and are... Adult stem cells (ASCs) Also known as somatic stem cells, these multipotent stem cells are located ...

  11. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation...human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells, 2013. 31(3): p. 458...possible to reprogram differentiated cells into induced pluripotent stem cells (iPSCs) by the addition of reprogramming... somatic cells that are otherwise difficult to obtain. These can then be used to perform cell therapy ...in vitro. Find stem cell plasmids at Addgene Many sources of adult somatic cells have been probed for...Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic...stem cells from a small amount of human peripheral blood using a combination of activated T cells and ...

  12. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ... of the cell and/or they cannot tumble in 720°, i.e. they are on a membrane or in an organelle, then their...transcriptional reporter or as a general cytoplasmic label of cells, there's no problem. You're OK. However, if you ...(POI) to GFP to study the protein's behavior in cells, in solution or something in between, you are using...fluorescent protein (FP) could be forming dimers in cells. Why should you care? Three simple ways a dimeric...nanomolar Kd will exist primarily as a dimer in cells while a protein with a high micromolar or low millimolar...millimolar Kd is unlikely to form dimers in a cell. The Kd of EGFP is 0.11 mM [2]. Following the simple...might think EGFP is unlikely to form dimers in a cell. Unfortunately, things are not quite so simple. ...

  13. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ... rising intracellular calcium levels within the cell. When levels of Ca2+ are high in the cell, calcineurin...infection (MOI) during cell library construction to ensure that the majority of cells receive one gRNA. The... of a genetically-defined subset of cells. By labeling fewer cells in a population, it’s easier to visualize...lengths, the density of cells that are labeled can be altered to dense (45% of cells labeled), intermediate...or aging cell populations. Figure 3: Micrographs of wild type (top panels) and cells with high...barcodes (iBARs) in their loop region. After infecting cells with sgRNAiBAR library containing lentiviral vectors...high MOI, which is beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR library...

  14. Immunology Research Plasmids and Resources

    Type
    Collection
    ...foreign antigens to adaptive immune cells, such as T cells and B cells. About a week following the initial...presenting cells. B cells in secondary lymphoid organs recognize specific antigens through the B cell receptor...have been described. NK Cell Signaling Natural killer (NK) cells can kill cells that have been infected... within an infected cell membrane, triggering cell death. TCR Signaling The T cell receptor (TCR) engages...through effects on numerous immune cell types including T cells and B cells. It also impacts the development...function of innate immune cells including NK cells, macrophages, dendritic cells, and granulocytes. TLR ...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...ATP (extracellular) ecAT3.10 extracellular ATP sensor (cell surface targeted) Imaging extracellular ATP... pH Imaging intracellular pH in live cells (pHRed) Imaging Intracellular pH in Live Cells with a Genetically...indicators FR-GECO1 Robert Campbell Lab (unpublished) Robert Campbell Calcium Yellow protein calcium sensor...SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep. 2019 Jun 25;27(13):...designed to specifically localize to cellular compartments or organelles. Addgene’s database includes many...in vitro vs. in vivo, cytoplasmic vs. extracellular vs. organelle-localized). Browse or search the tables...Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Nat Commun. 2020 ...

  16. Brain Initiative Collection

    Type
    Collection
    ... enhancer element Gordon Fishell 83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAVrg pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent...hDlx enhancer element Gordon Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...

  17. CRISPR Guide

    Type
    Collection
    ...with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID: ...in the same cell. This is called multiplexing , and increases the likelihood that any cell containing ... the cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...can increase efficiency. Synchronizing cells or modulating the cell cycle can help as HDR takes place during...expressing stable cell line. After applying your screening conditions to your transduced cell population, you...tracking of RNA in live cells, including orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview...promiscuity is unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the ...

  18. Antibody Guide

    Type
    Collection
    ... produced. Cells - Antibodies can also be produced in cell cultures using hybridomas, cell cultures created...created by fusing B cells with myeloma cells (immortalized B cells). The genetic reorganization that creates...identify and sort cells from a mixed population. Overview of application Collect cells from cell culture or ...specificity occurs in the B cell itself prior to antibody production; therefore, each B cell only produces one ... specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express...determines the isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can...mature B cell can clone itself to enable higher production of a single antibody, but each B cell can only...

  19. Plan Your Experiment

    Type
    Collection
    ...difficult-to-transfect cells. You can direct expression to specific cell types by using cell-specific promoters...human embryonic kidney 293 (HEK293) cells. For more difficult cell types, you may need to look into other...are good choices for more difficult cell types like primary cells. For CRISPR delivery, lentiviral and...deliver to target cells through microinjection or electroporation. The target cells translate the mRNA...complexes directly into your target cells. In this case, your target cells are not transcribing DNA to mRNA...possible genotypes within the resulting cell population. Some cells may remain wild type due to either (1...modifications . Cell Population The first step of validation is determining the type of cell population you...

  20. AAV for Neuronal Tracing

    Type
    Collection
    ...spread from this cell to synaptically connected cells (meaning, it can spread to cells that are projecting...infect a single "starter" cell, but new virus cannot spread to any neighboring cells. To enable monosynaptic...provided to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all the viral...projecting to the starter cell). Once in the secondarily infected cells that lack the G protein, the virus ...connected cells. To further refine the targeting of G-deleted rabies to specific starter cells, the tropism...G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein. EnvA-pseudotyped...is delivered to the starter cell, and specifically targets the starter cell via the EnvA receptor. RABV...

  21. Brain Armamentarium

    Type
    Collection
    ...channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213940-PHPeB ...expressing dTomato driven by PV+ basket cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213937-PHPeB ...channelrhodopsin-2 driven by chandelier cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213936-PHPeB ...expressing dTomato driven by chandelier cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213915-PHPeB ...Collection The Armamentarium for Precision Brain Cell Access is developing a collection of molecular genetic... reagents to gain access to many different brain cell types. The expanded collection of Armamentarium ...10aa-H2B-WPRE3-BGHpA (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG...

  22. Caltech Systemic Capsids

    Type
    Collection
    ...Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx mRuby2...Control Fishell 213829 pAAV_BiCHATe27_dTomato_nlsdTomato BiCHATe27 dTomato/nlsdTomato Control Fishell 213855...Control Fishell 213936 pAAV_BiPVe4_dTomato_nlsdTomato BiPVe4 dTomato/nlsdTomato Control Fishell 213940 ...Control Fishell 213944 pAAV_BiSSTe4_dTomato_nlsdTomato BiSSTe4 dTomato/nlsdTomato Control Fishell Chemogenetics...Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27 Activator ChR2 Fishell 213856 pAAV_BiVIPe4...Activator ChR2 Fishell 213915 pAAV_BiLAMP5e3_ChR2_mCherry BiLAMP5e3 Activator ChR2 Fishell 213937 pAAV_BiPVe4... Activator ChR2 Fishell 213941 pAAV_BiPVe3_ChR2_mCherry BiPVe3 Activator ChR2 Fishell 213945 pAAV_BiSSTe4...

  23. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...OPTN His CMV ALS Jon Ashwell 23051 pDEST26-hOPTN-D474N OPTN His CMV ALS Jon Ashwell 23052 pDEST15-hOPTN...hOPTN OPTN GST T7 ALS Jon Ashwell 23053 pDEST17-hOPTN OPTN His T7 ALS Jon Ashwell 23054 pDEST15-hOPTN-D474N...

  24. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . 155(7):1479-91. PMID: 24360272... for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander...Sontheimer EJ, Maxwell KL, Davidson AR. 2016. Naturally occurring off-switches for CRISPR-Cas9. Cell . 167(7)...described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more than...orthogonality, and multiplexibility in various species, as well as the development of new applications. The first...multiple loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR...Current libraries are available for gene knockout, as well as transcriptional activation or repression. Combined...

  25. Rett Syndrome

    Type
    Collection
    ...information Mouse and Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository...Unpublished Joost Gribnau Human Cell Line Models Induced pluripotent stem cells (iPSCs) are a powerful system...opens in a new window) LUHMES cell line , an immortalized pre-neuronal cell line that can be differentiated... RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines ...Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link ...X inactivation results in approximately half of cells expressing wild-type MECP2 and half expressing mutant...treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in a new window) JAX Mice for...

  26. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...to target a variety of cell populations in different brain regions. Labeled cell populations were tested...WPRE3-BGHpA AiP1379 AiE2150m SYFP2 Purkinje cells Cerebellum 208141 pAAV-AiE0390m_3xC2-minBG-SYFP2-WPRE3...regulatory sequences — termed enhancers — to drive cell type-specific expression. The Allen Institute for...differential chromatin accessibility across different cell types and cloned into an AAV backbone. Most enhancers...mouse brains. This collection contains plasmids for cell populations in the following regions: Isocortex ...Cortical subplate Striatum Pallidum Hypothalamus Cerebellum Whole brain Addgene ID Plasmid Allen Institute...-WPRE3-BGHpA AiP12320 AiE0475m SYFP2 Chandelier cells (Pvalb) Isocortex 230384* pAAV-AiE0475m-minBG-iCre...

  27. Lentiviral Prep Service

    Type
    Collection
    ...carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes...barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses...carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes...carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes...knockout pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone...

  28. Zebrafish Plasmid Collection

    Type
    Collection
    ...Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies on genetic... and track clones of cells over time. Examples of useful tools for studying cells that share clonal origin...Shendure Lab Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain - Alex...Mumm Lab. NTR 2.0/MTZ-based cell ablation system that allows sustained cell loss and ablation of ...below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation - Jeffrey...Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan...aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live imaging tools to study the development...

  29. Viral Production

    Type
    Collection
    ... preparations are added to cells in culture. Three to five days later, cell cultures are inspected for... a new vial of early passage cells. Approximately 2 weeks post-thaw, cell culture supernatant is tested...involves transducing cells with serial dilutions of the lentiviral vector, harvesting cells several days later...see maps section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre... (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445...Production Lentiviral preparations are produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging...packaging system developed by the Trono lab . Cell culture medium (OptiPro SFM) containing lentivirus is ...

  30. TALEN Plasmids and Kits

    Type
    Collection
    ...effectors to visualize subcellular positioning of target DNA sequences in living cells (TALE-mediated Genome...serial ligation. Validated in zebrafish somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate...ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru/Cowan Lab TALEN Kit Kit comprised...pC-GoldyTALEN Golden Gate TALEN 2.0 36033 pTAL5-BB Tom Ellis Plasmids pTAL5-BB and pTAL6-BB are designed as alternative..., both in microinjected embryos and transfected cells. TALEN expression is driven by the strong CAG promoter...destination vectors and a reporter vector for mammalian cell-based validation assay that are designed for use... the evaluation of TALEN activities in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate...

  31. Neurodegeneration Research Collection

    Type
    Collection
    ...protein-protein interactions and nerve cell function within the cell. Parkinson's Disease Parkinson’s disease...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of ...with the disease and implicate a wide range of cellular processes. The vast majority of cases (90-95%)...that causes the progressive degeneration of nerve cells in the brain. Symptoms include changes in behavior...emotion, uncontrolled movements, memory deficits, as well as difficulty speaking and swallowing. HD is caused...FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere et al. Mol Neurodegener. 2023 Aug 8...system, peripheral nervous system, vesicular brain cells and more. New and Noteworthy: AAV.rTH.PI.Cre.SV40...

  32. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging in Drosophila cells 3.3 MB...following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System The Förstemann... Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has produced...tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell lines were created using CRISPR and...methods. Several different tagging techniques, as well as the plasmids and protocols needed for using them...tagging transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use...guide the Cas9 protein to your target sequence, as well as design and clone in the homology arms for the...

  33. Viral Vectors

    Type
    Collection
    ...hard-to-transfect mammalian cells. In addition, "viral pseudotyping" can be used to target specific cells or tissues. ...genetic material into a cell, and even deliver this genetic material to specific cells in an organism. Figure...Genome Packaging Capacity Virus Size (nm) Cells Infected Target Cell Genome Integration Immune Response Lentivirus...because of their ability to transduce non-dividing cells. These viruses enable long-term stable gene expression...ability to preferentially infect and kill cancer cells. Adenoviruses have high transduction efficiency ... though this infectivity is limited to dividing cells. Retroviruses integrate permanently into the host...for the delivery of this genetic information to cells. Adapting plasmid DNA for use with virus-mediated...

  34. COVID-19 Resources

    Type
    Collection
    ... of the viral spike (S) protein to cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV...SARS-CoV-2 . TMPRSS2 - Hoffmann M, et al, 2020, Cell SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is...key step is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed...Human Angiotensin Converting Enzyme 2) is the host cell receptor mediating the entry of SARS-CoV and SARS-CoV...2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme that cleaves precursor ...2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine endosomal protease...increasing viral infectivity.( 6 ) PCP4 - (Purkinje cell protein 4) is involved in beating of airway cilia...

  35. Zhang Lab CRISPR Page

    Type
    Collection
    ...genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ., Cell 2013, Ran et al ., Nature...Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016/j.cell.2013.08.021. Epub 2013 Aug...Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014. Epub 2014 Sep... combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming...CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can ...

  36. Retrograde AAV viral preps

    Type
    Collection
    ...-GFP-Fishell-1 Dlx GFP Control Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control Fishell 83894...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hDlx Inhibitor DREADD Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hDlx Activator...83894 pAAV-hDlx-Flex-dTomato-Fishell_7 Dlx dTomato Control Fishell 104055 pAAV-CAG-eYFP CAG EYFP Control...83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 mDIx Activator Optogenetics Fishell 65014 pAAV-hsyn-Jaws-KGC-GFP-ER2... of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Harvey 137164 pAAV-nEF-Con/Fon/Von eYFP...Cre-dependent Calcium sensor Ting 83899 pAAV-mDlx-GCaMP6f-Fishell-2 mDlx GCaMP6f expression under the control of ...of the mDlx enhancer element Calcium sensor Fishell 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s...

  37. Deisseroth INTRSECT Collection

    Type
    Collection
    ... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions...to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND Flp,... Motifs. Cell Rep. 28(13):3450-3461. PubMed (Link opens in a new window) Mingote S, Amsellem A, Kempf ... powerful molecular reagents for biology have propelled our understanding of neural circuit functionality...parameters. Figure 1: Examples of intersectional cell population definitions using recombinases. A) Cre...adeno-associated virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic...multiple recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...

  38. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...and display of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac... the elements needed for replication in the host cell and are designed for a user to insert a gene of ..., or express the insert they carry in the target cell. These vectors allow you to test the function of....1-mCherry - Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible...or Fusion Protein Tags and fusion proteins are excellent tools for further understanding the function... as GFP, which allows you to visualize the cellular localization of your protein. Additionally...experiments, scientists often want to create stable cell lines in which the expression cassette of interest...

  39. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Amber N1 Yellow Mammalian Express a gene of interest fused to the N-terminus of Amber Amber C1 Yellow Mammalian...Venus pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses...protein interactions or conformation changes, as well as FRET standards. Plasmid...Protein Resources: Empty Backbones Biosensors Subcellular localization Optogenetics Background Förster ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses...interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest fused to the... the N-terminus of monomeric Venus mVenus C1 Yellow Mammalian Express a gene of interest fused to the ...

  40. Bacterial Expression Systems

    Type
    Collection
    ...organism due to its ease of use, speed of growth, and well understood biology. Other bacterial models, like... location of your protein of interest within the cell, you will need to tag it with something that makes...fused to FPs and monitor their localization in live cells. ID Plasmid Tags PI Additional Addgene Protein Visualization... and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for... inside living or fixed bacterial and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent Dyes...donor) tdTomato (acceptor) FRET/Dual FRET Robert Campbell Michael Davidson , Nathan Shaner , Roger Tsien...donor) mCitrine (acceptor) FRET/Dual FRET Robert Campbell , Michael Davidson 54571 54856 mT-Sapphire-pBAD...
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