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We narrowed to 163 results for: NAP

Showing: 41 - 80 of 163 results

  1. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells Note: This is not an extensive...fluorescently label antibodies, and purification is a snap using thin layer chromatography (Fessenden, 2009...

  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... a particular neurotransmitter release or post-synaptic current. Fortunately, if your lab focuses primarily...expressing, generic neuronal promoters such as synapsin or camKIIα drive most of the constructs. As an...neuronal death if allowed to express for too long (synapsin was privately communicated to me as one of these...

  5. Brain Initiative Collection

    Type
    Collection
    ...optogenetics. Human Synapsin (hSynapsin) promoter Edward Boyden 107712-AAV9 pAAV-hSynapsin-FLEX-soCoChR-GFP...-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA... viral vector Hillel Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which...Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1...Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter Lin Tian 111068-AAV5 pAAV-hSyn-dLight1.2...Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter Lin Tian 111069-AAV1 pAAV-CAG-dLight1.2...

  6. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human...101129 pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control...Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag Control...Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression...expression under T7 control 101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient...transient mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box)... localization Supplemental Information SNAP and CLIP tags SNAP and CLIP tag FAQs (PDF, 252 KB) ACP and...

  7. Biosensor AAV Preps

    Type
    Collection
    ...111262 pAAV-hSynapsin1-axon-GCaMP6s Syn axon-GCaMP6s none Constitutive 1 Tian 112000 pAAV-hSynapsin1-axon-GCaMP6f...HT Promoter CAG CaMKIIa Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent...Serotype PI Acetylcholine Sensors 137950 pAAV.hSynap.iAChSnFR Syn iAChSnFR none Constitutive 1 Looger ... none Constitutive 1, 9, rg* Fyhn 172921 pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 Syn axon-jGCaMP8s mRuby3...mRuby3 Constitutive 1, 9 GENIE 172923 pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 Syn axon-jGCaMP8f mRuby3... none Constitutive 1, 9, rg* Fyhn 172922 pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 Syn axon-jGCaMP8m mRuby3... Constitutive 1, 5, 9 Kim , GENIE 111261 pAAV-hSynapsin1-axon-GCaMP6m Syn axon-GCaMP6m none Constitutive...

  8. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...400,000 cells were stained with the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite...You can see an example of their work on their synaptotagmin 1 page. The data produced is available on the...to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot...

  9. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...channel Mouse Mouse IgG2a 140071 Anti-Snapin/SNAPAP [L8/15R] Snapin/SNAPAP Human Mouse IgG2a 140072 Anti-Mitofusin... 177471 Anti-Synapsin-3 [L125/121R] Synapsin-3 Human Mouse IgG2a 177472 Anti-Pan-Synapsin [L125/129R] ... Human Mouse IgG2a 114476 Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a 114477 Anti-TRPML3... Human Mouse IgG2a 114482 Anti-Synaptotagmin-12 [N277/7R] Synaptotagmin-12 Mouse Mouse IgG2a 114483 Anti-GluA1...) Rat Mouse IgG2a 114558 Anti-Synaptotagmin-10 [N269/73R] Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin... Mouse Mouse IgG2a 177526 Anti-Synaptotagmin-7 [N275/14R] Synaptotagmin-7 Mouse Mouse IgG2a 177527 Anti-Lgi1... Human Mouse IgG2a 188216 Anti-Synaptotagmin-6 [N270/47R] Synaptotagmin-6 Mouse Mouse IgG2a 188217 Anti-CASK...

  10. AAV for Neuronal Tracing

    Type
    Collection
    ...Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools for Monosynaptic Neuronal...Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with modified...not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype...Wickersham Introduction to Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect... within infected cells, but is required for transynnaptic spread (Wickersham et al., 2007a, Wickersham...cannot spread to any neighboring cells. To enable monosynaptic spread of this deletion-mutant rabies, the essential...

  12. AAV Molecular Tools

    Type
    Collection
    ... constitutive Expression of pre-synaptic mGRASP-mCerulean for synaptic connectivity mapping 5 Kim 34912... constitutive Expression of post-synaptic mGRASP-dTomato for synaptic connectivity mapping 5 Kim Tools...tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator...pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of the...Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP for labeling of axon terminals. 1 Zeng ...Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression ...expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals. 1 Luo 60658 pAAV-EF1α-...

  13. Genetic Code Expansion

    Type
    Collection
    ...Schultz 48696 pANAP AnapRS E. coli 3-(6-acetylnaphthalen-2-ylamino)-2-amino-propanoic acid (Anap) Mammalian...4xEcoLeuT(CUA)_AnapRS AnapRS E. coli 3-(6-acetylnaphthalen-2-ylamino)-2-amino-propanoic acid (Anap) Mammalian...Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF) pyrrolysine (Pyl...Bacterial TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (...Mammalian Elizabeth Kirby 223508 pCMV_NESPylRS(AF)_hU6tRNAPyl PylRS (Y306A, Y384F) M. mazei Mammalian TAG Edward... Edward Lemke 223509 pCMV_NESPylRS(WT)_hU6tRNAPyl PylRS M. mazei Mammalian TAG Edward Lemke 223511 pEVOL_PylRS...

  15. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive Cre expression...AAV. Because Cre expression is controlled by the synapsin promoter, it and therefore mCherry will only be...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...membrane Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal...hippocampal synapses. Neuron. 2006 Sep 21. 51(6):773-86. Leon Lagnado Endo/Exocytosis Synaptophysin with tandem...2023.07.18.549527 Eric Schreiter Calcium GCEPIA1-SNAP ER for sensing ER calcium A genetically encoded ...sensitivity and scale (4s) or speed (4f) of imaging synaptic transmission Glutamate indicators with increased...glutamate sensors (iGluSnFR3) for improved imaging of synaptic transmission Glutamate indicators with improved...activation kinetics and localization for imaging synaptic transmission. Nat Methods. 2023 May 4. Kaspar ... high-frequency release at Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115...

  18. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV...all our GABA sensor AAV inventory here. pAAV.hSynap.iGABASnFR (112159-AAV1) Optogenetics Optogenetics...

  20. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...turnover within a neuron? For my PhD I studied a synaptic protein that is linked to neurodegeneration. The...normal cell function, and, because I was studying a synaptic protein, I wanted to measure it’s levels in its...

  23. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Claudio Alarcon 200789 CLIPf-KIF5A-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...ALS Alison Twelvetrees 200791 KIF5A-SNAPf-HisTag-FLAG KIF5A SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...200792 CLIPf-KIF5A(delta954-1027)-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...CLIPf-KIF5A(1-914; delta505-610)-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...Twelvetrees 200798 CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...MBP, TEV, SNAP PH ALS Anthony Hyman 221886 TH0994-pOCC177-Fus-wt_(1-211) FUS His, MBP, TEV, SNAP PH ALS ... TEV, SNAP PH ALS Anthony Hyman 221888 TH1149-pOCC177-FUS(Nhe)-RGG_RtoK FUS His, MBP, TEV, SNAP PH ALS...

  24. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...the presence of a specific protease inhibitor, asunaprevir, the viral protease can no longer self-cleave...components are 1) a SMASh-tagged protein and 2) asunaprevir (small molecule). dTAG The degradation tag (dTAG...

  25. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

  26. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...a sequence manager A sequence manager, such as SnapGene, can be very helpful to confirm primer design....currently investigating the molecular biology of synaptic transmission and plasticity.     Javier García-Nafría...

  27. Hot Plasmids - November 2023

    Type
    Blog Post
    ...is a scaffolding protein known for its roles in synapse development, transmission, and plasticity and is... is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD-95 [K28/43R] has been...

  28. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...expertise to our mutual benefit.  Our partnership with SnapGene, for example, allows us to display high-quality... into it. At the same time, this project gives Snapgene great exposure to the community of biological ...

  30. Important Considerations When Using AAVs

    Type
    Blog Post
    ... neurons (retrograde transport) or towards the synapse (anterograde transport), which is a possible mechanism... storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen or a dry ice/ethanol bath...

  31. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Optogenetics Karl Deisseroth AV-1-26967M 100049-AAV1 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Optogenetics Karl...Optogenetics Karl Deisseroth AV-9-26967M 100049-AAV9 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Optogenetics Karl...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...CI.eGFP.WPRE.rBG James M. Wilson AV-2-26967M 100049-AAV2 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Karl Deisseroth...floxed-eNpHR-EYFP-WPRE-pA Karl Deisseroth AV-5-26967M 100049-AAV5 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Karl Deisseroth...

  33. Optogenetics AAV Preps

    Type
    Collection
    ...Bidirectional BiPOLES Promoter CAG CaMKII CBA Dlx EF1a/nEF Synapsin E2 regulatory element Fluorophore GFP Red-wavelength...EYFP Cre dependent PHPeB Gradinaru 107708 pAAV-hSynapsin- soCoChR-GFP Syn CoChR (soma-targeted) GFP Constitutive...Constitutive 9 Boyden 107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn CoChR (soma-targeted) GFP Cre dependent...ChrimsonR tdTomato Flp dependent 8 Boyden 100049 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Syn ChETA EYFP ...

  34. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...developed for their use with rabies virus (RABV) in monosynaptic retrograde tracing of neurons. In this system...express a viral element necessary for RABV trans-synaptic transport. Learn more about these and other initiatives...for neuronal tracing . Browse AAV vectors for monosynaptic neuronal tracing available at Addgene. Optogenetics... from the main cell body, or soma, towards the synapse at the axons. Blood-brain barrier (BBB) Layer of... of molecules, vesicles or organelles from the synapse at the axons towards the main cell body, or soma...

  35. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Erik Snapp 49150 BFP-KDEL...GalT signal anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N-10 Golgi apparatus MannII...

  37. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ... Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your labmates celebrating the...

  39. Call for Guest Bloggers

    Type
    Blog Post
    ...Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein College ...

  40. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ... its bistable nature, it enables inhibition of synapses distant to the site of injection with high temporal...
Showing: 41 - 80 of 163 results