We narrowed to 817 results for: cell

...Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging in Drosophila cells 3.3 MB...following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System The Förstemann... Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has produced...tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell lines were created using CRISPR and...tagging transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use... from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly... CRISPR tagging technique for use in Drosophila cells that uses PCR to generate both an expression cassette...

...use electro-competent cells instead of the more common chemically-competent cells. The number of bacterial... manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-...1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total...colonies, you might want to use higher competency cells. Additionally, if your final product is going to...

...genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . 155(7):1479-91. PMID: 24360272... for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander...described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more than...multiple loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR...genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific flanking...proteins can be used to image genomic loci in live cells. RNA targeting : Cas13 enzymes target RNA rather...non-specific cleavage does not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used ...

...integrase to insert the viral genome into a host cell. Scientists can take advantage of this to stably...integrate transgenes of interest into a variety of cell types. Wild-type gamma-retroviruses have been engineered...of Large T antigen for creation of immortalized cells. See the Weinberg Lab page for additional resistance...Expression of hTERT for creation of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid...inducible expression of GFP T2A Cre fusion in mammalian cells. Floris Foijer 47916 pRXTN CMV/MSV A modified version...MoMSV Stable expression of luciferase in mammalian cells. Alice Wong 83356 pMXs-3XHA-EGFP-OMP25 MoMSV For...tagging mitochondria with HA epitopes in mammalian cells. David Sabatini Return to top Popular Gamma-Retroviral...

...molecular switches that transmit signals within the cell. GTPases have an active (GTP) and inactive (GDP)...prominent roles in signaling pathways regulating cell migration, survival, adhesion, growth, and differentiation...Cancer Impact Ras is ubiquitously expressed in all cells and all tissues. Ras can activate a variety of ...pathways, which ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential...essential for regulating the growth of the cell, however when mutations occur in the Ras gene it can be... active form, constantly activating pathways for cell growth and potentially leading to cancer. Constitutively...E2F2 E2F3 E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor...

...access to cortical cell types. Cell , 188 (11), 3045–3064. https://doi.org/10.1016/j.cell.2025.05.002 PMID...to target a variety of cell populations in different brain regions. Labeled cell populations were tested...regulatory sequences — termed enhancers — to drive cell type-specific expression. The Allen Institute for...differential chromatin accessibility across different cell types and cloned into an AAV backbone. Most enhancers...mouse brains. This collection contains plasmids for cell populations in the following regions: Isocortex ...-WPRE3-BGHpA AiP12320 AiE0475m SYFP2 Chandelier cells (Pvalb) Isocortex 230384* pAAV-AiE0475m-minBG-iCre...pAAV-AiE0475m-minBG-iCre(R297T)-BGHpA AiP13790 AiE0475m Cre Chandelier cells (Pvalb) Isocortex 214561 pAAV-AiE2566m-minBG-SYFP2...

...compatible with fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting... multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence... for detecting the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for... loci, as well as chromosome painting in living cells. Visualizing a specific genomic locus requires recruitment...repetitive loci can be efficiently visualized in living cells using a single gRNA that has multiple targeted sequences...

...adipose tissue-derived mesenchymal stem cells into pluripotent stem cells by a mutant adeno-associated viral...serotype also demonstrates enhanced transduction of cells in culture for ex-vivo conditioning. The AAV2(trpYF...displays enhanced transduction of hematopoietic stem cells and microglia. The AAV6(dbY-F+T-V) serotype was ...AAV-mediated transduction and targeting of retinal bipolar cells with improved mGluR6 promoters in rodents and primates...human gene therapy of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 Apr;27(3...delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of... of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation...

...Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID... (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine/threonine...signals to determine the nutritional state of the cell and regulates the switch from anabolic to catabolic...growth factors, including insulin. mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact... promotes the conditions needed for uncontrolled cell growth and proliferation, including an abundant ...appears to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids... one drug, many effects. Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3):373-9. doi: 10.1016/j.cmet...

...expression systems including E. coli , mammalian cells, and cell-free systems. NanoBiT Assays NanoLuc Binary... to either LgBiT or SmBiT and expressed in live cells. When the two proteins interact, the subunits come...proteins at endogenous levels in lysates or live-cells, thus preserving physiological relevance. NanoBRET...designed to measure molecular proximity in living cells. NanoBRET PPI assays use NanoLuc Luciferase as a...NanoBRET 618 fluorophore, as the energy acceptor. Live-cell reagents allow you to detect PPI induction and inhibition... NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase...NanoLuc fusions are useful for both intracellular and extracellular applications where you want minimal...

...Jinhyun Kim Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ID Name Expression...Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression...expression of designer pro-taCasp3 and TEVp for studying cell ablation. 1, 5, 8 Nirao Shah , Jim Wells 124364 ...diphtheria toxin receptor fused to GFP for studying cell ablation. 2 Thomas Jessell , Eiman Azim 207997 pAAV...Tools These AAV encode tools that can be used for cell or protein labeling. ID Name Expression System Activity...AAV8-DIO-Reporter viruses infected in postsynaptic cells. 5, 8 Don Arnold 232352 pAAV-DIO-ATLASsnFlp Syn-...-fDIO-Reporter viruses infected in postsynaptic cells. 5, 8 Don Arnold 232353 pAAV-DIO-BACE-HA Syn-driven...

... Protease Inhibitor . Cell, 181 (2), 271–280.e8. https://doi.org/10.1016/j.cell.2020.02.052 (Link opens...virus. SARS-CoV-2 entry into a host cell, like that of SARS-CoV and MERS CoV, depends on ...key step is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed...Additional Addgene resources ACE2 plasmids - Host cell receptor mediating the entry of SARS-CoV and SARS-CoV...2 S protein and is involved in virus entry into cells (Hoffman et al., 2020). FURIN plasmids - an enzyme...2 S protein and is involved in virus entry into cells (Wang et al., 2020). Cathepsin L plasmids - a cysteine...Heurich et al., 2014). PCP4 plasmids - (Purkinje cell protein 4) is involved in beating of airway cilia...

...function in zebrafish, plants, and human cells ( Maeder et al., Mol Cell 2008 , Foley & Yeh et al., PLoS ONE... Townsend et al., Nature 2009 , Zou et al., Cell Stem Cell 2009 ). Resources A detailed protocol for practicing...ZFNs that function in zebrafish, plants, and human cells Kits...

...Kits Allen Institute for Cell Science Plasmid Collection New England BioLabs Cell-Imaging Plasmid Collection...activate, inhibit, and control molecular pathways or cells. Chemogenetics Guide | Chemogenetics Plasmids Use...activate or inhibit engineered receptors that affect cell signalling pathways. Luciferase Plasmids Use bioluminescent...physiological intracellular processes with genetically encoded fluorescent biosensors. Subcellular Localization...Localization Visualize organelles and subcellular structures with well-characterized marker proteins. In Vivo...

...between animal cells. They are important for holding cell structures together and reducing cell stress. There...give the cell its shape, organize cellular organelles, and are necessary for movement and cell division...example, houses the cell’s genetic material. The mitochondria produces the energy cells required for chemical... on cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle...Fluorescent Protein Collection . Return to top Cell Junctions Cell junctions are responsible for forming connections...General Structures Eukaryotic cells contain a variety of organelles and subcellular structures, each with their...Return to top Cytoskeleton The cytoskeleton is the cell's internal framework that assists in sustaining structure...

...proliferating cells. Expression may cause repetitive recombination leading to genomic instability and cell death...and control the expression of specific genes in cells or organisms. Each recombinase enzyme recognizes... the enzyme at specific times or locations using cell-specific promoters or inducible systems, you can...promoters. Expression of both fragments in the same cell reconstitutes a functional protein, allowing recombination...expression. For example, iCre or FLPo in mammalian cells may facilitate higher rates of recombination. Gene...Flp-IN: A single FRT site within the genome of a host cell line serves as the target location for integration...expression vector allows you to visualize which cells have undergone recombination. Reporter plasmids ...

...barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses...carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes...carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes...carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes...Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version 1 Ready-to-use lentiviral...

...and display of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac... the elements needed for replication in the host cell and are designed for a user to insert a gene of ..., or express the insert they carry in the target cell. These vectors allow you to test the function of....1-mCherry - Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible...experiments, scientists often want to create stable cell lines in which the expression cassette of interest...is incorporated into the host DNA. For mammalian cells, you can do this through viral vector delivery...transduce both dividing and nondividing cells, integrate into host genome pLenti CMV GFP DEST ...

...expression of the transactivator proteins in mammalian cells or other species by codon optimization of their ...gene of interest from two separate vectors. Stable cell lines or transgenic animals can be made to achieve... and ensures that all transfected or transduced cells express both components in the same relative ratio...derivatives can be found in Krueger et al., 2004. Some cell culture sera may contain tetracycline or its derivatives...expression of mouse Oct4, Sox2, Klf4, and Myc for iPS cell generation Rudolf Jaenisch 51543 FUW-tetO-hOKMS ...expression of human Oct4, Sox2, Klf4, and Myc for iPS cell generation Tarjei Mikkelsen 172115 PB-TO-hNGN2 Piggybac...pSF3-ultraID Expression of UltraID in mammalian cells with the Tet-On or Tet-Off system Julien Béthune...

...then cell type. For CNS: For infant rhesus macaques: Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased...tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For rats...tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1, AAV1-X1...including: the delivery method, mouse strain, target cell type or tissue of interest, and model organism under... above helps you choose the best capsid for your cell type. First, do you want to target CNS ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE or...AAV-PHP.eB with GRE or transgenic animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10, AAV-...

...mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the.... This buffer is not compatible for use in live cells and will interfere with conjugation. For applications...antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were performed...email [email protected] . Immunocytochemistry (ICC) Cells are fixed and labeled with the antibody and a species-specific...Western Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE, transferred to a membrane...

...expressing these in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between... research purposes include animals or hybridoma cells, but are difficult to generate due to expense and...monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived from an individual...transiently transfected into mammalian tissue culture cells, the R-mAbs are secreted into the culture media ...

...genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ., Cell 2013, Ran et al ., Nature...Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016/j.cell.2013.08.021. Epub 2013 Aug...Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014. Epub 2014 Sep...CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can ...recombination and fluorescence assisted sorting of cells and nuclei in addition to sgRNA expression. This...

... Modeling Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids Matthew Meyerson Dana-Farber Cancer...Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University...University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute...

...Institute for Cell Science Plasmid Collection E11 Bio PRISM Collection New England BioLabs Cell-Imaging Plasmid...Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase CUREs ...

...important to normal cell signaling and physiology, and, when dysregulated, can enable cancer cell survival. Although...that is essential for regulating the growth of the cell. p53 p53, the most frequently mutated gene in human...

...Cas protein and a target-specific gRNA in the same cell at the same time. All-in-one plasmids ensure that...both the Cas and gRNA are delivered to the same cells, but are limited to the function of the Cas protein...an additional plasmid containing a Cas enzyme or cells or organisms in which Cas has been integrated into...

...to activate programmed cell death or dormancy for phage-infected bacterial cells so as to limit the spread...population. Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian...

...to blue light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered...improve the expression and membrane targeting in host cells. Other microbial opsins, such as the Halorhodopsins...actively move cations or anions into or out of the cell. When using these opsins as a tool to control neuronal...switch tools to activate, inhibit, and manipulate cellular processes. Plasmid...plasmids that use light to control a protein or cellular activity. For example, search "Cdc42", "transcription...

...Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells ( Miller et al., Nat Biotechnol. 2011 ; Reyon and...., Nat Biotechnol 2012 ) Human pluripotent stem cells ( Hockemeyer et al., Nat Biotechnol. 2011 ) TALE...TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013 ) More detailed information...

... location of your protein of interest within the cell, you will need to tag it with something that makes...fused to FPs and monitor their localization in live cells. ID Plasmid Tags PI Additional Addgene Protein Visualization... and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for... inside living or fixed bacterial and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent Dyes... Siouxsie Wiles 106476 pET28a_T5-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia...Mikhail Shapiro 106473 pET28a_T7-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia...Mikhail Shapiro 106474 pET28a_T7-ARG2 Bacterial cells Ultrasound (acoustic reporter gene, ARG2) Escherichia...

...collection of synthetic biology plasmids related to cell-to-cell communications and environmental sensing. Sensing...

...or genetic elements of interest to introduce in cells. A packaging plasmid (or Rep/Cap plasmid) containing...or herpes simplex virus, to replicate in the host cell and complete the lytic cycle. To avoid the use of...provided by helper plasmids and specific packaging cell lines. Helper plasmids generally encode the adenoviral... General Transfection AAV Production in HEK293T Cells AAV Purification by Iodixanol Gradient Ultracentrifugation...

...p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose ...or E2 Cyclin G CCNG1 CCNG2 Cyclin G1 or G2 CDC25 Cell division cycle 25C Cdk4/6 CDK4 CDK6 Cyclin-dependent...member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 GADD45A GADD45B GADD45G...transcription factor and tumor suppressor activated by cellular stress. Cancer...transcription factor and tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and...

...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...firefly luciferase to simultaneously measure the # of cells containing reactivated latent provirus and the overall...strength of viral transcriptional response in these cells. Warner Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral...

...in which the cell line was described. Using Cellosaurus Cellosaurus is searchable by cell line name (recommended...resource called Cellosaurus, a growing cell line information database describing nearly 145,000 cell lines. Cellosaurus... related cell lines when annotating a cell line Community submissions via email: cellosaurus@sib.swiss...with cell line catalogs and collections. Importantly, Cellosaurus also identifies problematic cell lines...HeLa cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus Generated a new cell? Or ...problems with cell lines. Science 2014; 346:1452–1453. Bairoch, A. The Cellosaurus, a Cell-Line Knowledge...problems, like cells lines. A 2014 study in Science found that approximately one-third of cell lines used ...

...Tracking lineage and cell identity at the single-cell level with CellTag CellTagging is a lentiviral based...individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. ...reprogrammed cells based on single-cell gene expression patterns. The lab has also used CellTags for tagging...describes the process where differentiated cells are turned into a cell type of choice while bypassing the intermediate...majority of cells failing to develop the desired identity. The development of single cell technologies...simultaneously tracks clonal history and cell identity at the single-cell level, thus linking ancestral gene...), a self-renewing cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed...

...Expression of firefly luciferase from a cell-free mammalian cell lysate Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE...not secreted, so cells must be lysed or the substrate must be delivered intracellularly to measure luminescence...expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 212936 pGL3 Basic Vector Firefly... viral infection efficiency, to study changes in cell physiology, or as a reference level of expression...Expression of Gaussia luciferase; GFP is expressed if cells are infected with virus Christopher Buck 98580 pLX304...membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc ...serve as sensors of proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (...

...to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND Flp,...parameters. Figure 1: Examples of intersectional cell population definitions using recombinases. A) Cre...adeno-associated virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic...Neve R, Huang ZJ, Deisseroth K. 2014. Targeting cells with single vectors using multiple-feature Boolean...Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed (Link opens in a new...well-defined cellular sub-populations has generally been limited to single-component cellular definitions...multiple recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...

...complete plasmid can be transformed into bacterial cells for propagation. The majority of ligation reactions...Interpretation Uncut vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid...