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We narrowed to 85 results for: psin

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Showing: 41 - 80 of 85 results

  6. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...

  8. Hot Plasmids - October 2020

    Type
    Blog Post
    ...a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now available as AAV5. Find the AAV...

  11. Common Injection Routes in Mice

    Type
    Blog Post
    ... place the needle in the correct vein without collapsing it. Retro-orbital injections are also thought...

  14. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...they determined that an A122D mutation in the rhodopsin domain conferred several new improvements. The...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...to express either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the...

  18. Brain Initiative Collection

    Type
    Collection
    ...optogenetics. Human Synapsin (hSynapsin) promoter Edward Boyden 107712-AAV9 pAAV-hSynapsin-FLEX-soCoChR-GFP... Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell...AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and ...pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics....pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted...Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1...

  21. Optogenetics Plasmid Collection

    Type
    Collection
    ...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps... microbial opsins to be adopted as an optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from...Find optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and...Plasmids Optogenetics Plasmid Collection Microbial Opsin Plasmids Optical Switch Plasmids Additional Resources...light. Many of these plasmids encode for microbial opsins, which are membrane channels and pores derived ...cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified to increase...anions into or out of the cell. When using these opsins as a tool to control neuronal activity, scientists...

  22. Brain Armamentarium

    Type
    Collection
    ...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer...

  23. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-hSynapsin1-axon-GCaMP6s Syn axon-GCaMP6s none Constitutive 1 Lin Tian 112000 pAAV-hSynapsin1-axon-GCaMP6f...LiLac Promoter CAG CaMKII Dlx EF1a GFAP/GfaABC1D Synapsin Other Activity Cre-dependent Flp-dependent Cre...Constitutive 1, 9, rg* Marianne Fyhn 172921 pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 Syn axon-jGCaMP8s mRuby3...mRuby3 Constitutive 1, 9 GENIE Project 172923 pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 Syn axon-jGCaMP8f mRuby3...none Constitutive 8 Marianne Fyhn 172922 pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 Syn axon-jGCaMP8m mRuby3... 5, 9 Douglas Kim , GENIE Project 111261 pAAV-hSynapsin1-axon-GCaMP6m Syn axon-GCaMP6m none Constitutive...axon-GCaMP6f none Constitutive 1 Lin Tian 112005 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 Syn axon-GCaMP6s bicistronic...

  25. Optogenetics AAV Preps

    Type
    Collection
    ...optogenetics AAV collection, including channelrhodopsins, halorhodopsins, and more....optogenetics plasmids available in Addgene's collection. Opsin Excitatory Wild-type ChR2 ChR2/H134R soCoChR ChR2...Arch and variants eNpHR and variants InSynC (non-opsin) Jaws WiChR stGtACR iC++ OPN3 PdCO PPO Bidirectional...CaMKII CBA Dlx E2 regulatory element EF1a/nEF GFAP Synapsin TRE Fluorophore GFP Red-wavelength fluorescent...AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2...dependent PHPeB Viviana Gradinaru 107708 pAAV-hSynapsin- soCoChR-GFP Syn CoChR (soma-targeted) GFP Constitutive...Constitutive 9 Edward Boyden 107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn CoChR (soma-targeted) GFP Cre dependent...

  27. Lentivirus ddPCR Titration

    Type
    Protocol
    ...cations can affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol,...

  28. Chemogenetics Plasmids

    Type
    Collection
    ... developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting... light-sensing optogenetic element such as channelrhodopsin. When the luciferase enzyme oxidizes its substrate...luciferin), it generates light that activates the opsin to either excite or inhibit the target neuron. LMO...

  29. AAV Molecular Tools

    Type
    Collection
    ...Viviana Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator...pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of the...HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev 200778 AAV-∆Hapln1...and fused with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev 203840 pAAV-hSyn-Kir2.1WT...

  30. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... 177471 Anti-Synapsin-3 [L125/121R] Synapsin-3 Human Mouse IgG2a 177472 Anti-Pan-Synapsin [L125/129R] ...Neuroligin-2 Mouse Mouse IgG2a 206608 Anti-Synapsin-3 [L125/76R] Synapsin-3 Human Mouse IgG2a 206610 Anti-Cav1.3...Mouse IgG2a 225366 Synapsin-3 (Homo sapiens) recombinant monoclonal antibody. Synapsin-3 Human Mouse IgG2a...Bassoon Rat Mouse 182079 pan-Synapsin scFv [L125/129] L125/129 pan-Synapsin Mouse 182080 Aldh1L1 scFv [...] Pan-Synapsin Mouse IgG2a 177473 Anti-SAPAP3 [L126/93R] SAPAP3 Mouse Mouse IgG2a 177474 Anti-GAD65/67...

  31. AAV Viral Preps

    Type
    Collection
    ...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...

  32. Deisseroth INTRSECT Collection

    Type
    Collection
    ...additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies... Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock... Cre Yes Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock...

  33. COVID-19 Resources

    Type
    Collection
    ...in virus entry into cells (Wang et al., 2020). Cathepsin L plasmids - a cysteine endosomal protease that.... J., Bartelink, W., & Rottier, P. J. (2008). Cathepsin L functionally cleaves the severe acute respiratory...

  34. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Ho 102364 pSIN-PAmCherry-mSNCA-NE SNCA NE EF-1 alpha Parkinson's Shu Leong Ho 102366 pSIN-hSNCA-NE SNCA...Mathea 227004 pAAV-CMVenh synapsin-intron-aSYNUCLEIN A53T SNCA CMVenh synapsin Parkinson's Veerle Baekelandt...Vallon 247940 pAAV-CMVenh synapsin-intron-aSYNUCLEIN WT SNCA CMVenh synapsin Parkinson's Veerle Baekelandt...209085 piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623) SNCA TRE Parkinson's Vikram ...piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628) SNCA Avi TRE Parkinson's Vikram...

  35. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...reporter VARNAM Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator. Neuron. 2023... mammalian neurons using engineered microbial rhodopsins. Nat Methods. 2014 Aug;11(8):825-33. Adam Cohen...D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based voltage imaging tools for use in muscles...

  36. Optogenetics Guide

    Type
    Guide
    ...non-neuronal cell types. Luminopsins Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic tools...Microbial Opsins and Variants Channelrhodopsins from C. reinhardtii Other Channelrhodopsins Anion Channels...Channels Halorhodopsins Archaerhodopsins Leptosphaeria Rhodopsins These lists are not exhaustive — check... Guide Jump To: Optogenetics Guide Opsins Glossary of Common Opsins Optical Switches Glossary of Common...cell (yellow dots), activating the neuron. Opsins Opsins are light-gated ion channels or pumps that absorb...trafficking signals to localize opsins to the cell membrane. Microbial opsins, such as those described below...some examples of relevant variants. Channelrhodopsins Channelrhodopsins are a foundational optogenetics tool...

  37. Luciferase Plasmid Collection

    Type
    Collection
    ...to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion proteins for bioluminescent ...

  38. Immunology Research Plasmids and Resources

    Type
    Collection
    ...1 CREB, MGC9284 CTSB cathepsin B APPS, CPSB CTSE cathepsin E CATE CTSL1 cathepsin L1 CATL, CTSL, FLJ31037...FLJ31037, MEP CTSS cathepsin S MGC3886 ERAP1 endoplasmic reticulum aminopeptidase 1 A-LAP, ALAP, APPILS, ...CHEMERINR, ChemR23, DEZ, MGC126105, MGC126106 CTSG cathepsin G CG, MGC23078 CX3CL1 chemokine (C-X3-C motif)...factor CCN2, HCS24, IGFBP8, MGC102839, NOV2 CTSG cathepsin G CG, MGC23078 CX3CL1 chemokine (C-X3-C motif)...

  39. Chemogenetics Guide

    Type
    Guide
    ...activation Magnus et al., 2011 Luminopsins Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic fusion... enzyme generates light that activates the opsin. The opsin may excite or inhibit the neurons expressing.... E. (2015). Inhibitory luminopsins: genetically-encoded bioluminescent opsins for versatile, scalable...-fluorescent protein fusions, to more-sensitive opsins has led to improved LMOs with increased efficiencies...systems in our Optogenetics Guide . Figure 4: Luminopsins can be activated through chemogenetic or optogenetic...LMOs and their activity in neurons LMO Luciferase Opsin Ligand Effect Outcome Reference LMO3 sbGLuc VChR1...., Yin, H. H., & Hochgeschwender, U. (2016). Luminopsins integrate opto- and chemogenetics by using physical...
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