We narrowed to 1,017 results for: LEA

1. Open Enzyme Collection

   Type

   Collection

   ...glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease I 165551 pOpen-Endonuclease IV E. coli E... E. coli Endonuclease IV 165561 pOpen-Endonuclease VIII E. coli E. coli Endonuclease VIII 165571 pOpen-Exonuclease...pOpen-Exonuclease III E. coli E. coli Exonuclease III 165559 pOpen-T5gene12/13 T5 Exonuclease 165560 pOpen-T4...Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-...

2. Fluorescent Protein Guide: Empty Backbones

   Type

   Collection

   ...Expression Cerulean 433 475 27 4.7 Monomer (A206K) Cerulean - Mammalian Expression pCS2+8CCerulean - Zebrafish...Expression mCerulean C1 - Mammalian Expression mCerulean3 433 475 32 3.2 Monomer (A206K) mCerulean3-N1 - Mammalian...Expression mCerulean3-C1 - Mammalian Expression moxCerulean3 434 474 36 3.2 Monomer (A206K) moxCerulean3 - Mammalian...C.elegans/Sea urchin pCS2+8NCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin mCerulean N1 - Mammalian Expression...Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and C-terminal... Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP pPD95_75 - C-terminal GFP for...cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial Vectors - Includes...

3. Distribution to Industry

   Type

   Collection

   ...Looking For? Please let us know what you would like to see available to industry scientists. Please note this...page for more information. To learn more about Addgene's MTA process please visit our Help Center . The ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to order...Addgene as well as by our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended...account, you can place orders through our website. Please note that all orders must be placed online through... product pages after you login to your account. Please see our Help Center for more information on pricing...language which we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer...

4. Plan Your Experiment

   Type

   Collection

   ...targets may not result in equivalent cleavage efficiency. In fact, cleavage efficiency may increase or decrease...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...decrease expression of a target gene? Once you have a clear understanding of your experimental goal, you are...also important to consider factors that maximize cleavage of the desired target sequence, or on-target activity...exhibits the lowest immunogenicity and are one of the least toxic methods for in vivo viral delivery. Additionally...Before proceeding, we recommend asking labmates/colleagues, searching the literature, or discussing with...Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and gRNA. Before...

5. TALEN Guide

   Type

   Collection

   ...a zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer...sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components of the...these tools and the researchers who are learning to wield them. Learn more about Addgene’s TAL effector and...years of research has shown that when fused to a nuclease or activation domain, a ZF array is an effective...at Massachusetts General Hospital also recently released a TALEN kit through Addgene. This kit, first described...

6. University of Florida Serotype Testing Panel for the Eye and Brain

   Type

   Collection

   ...available in each of the above serotypes and buffers. Please click on the plasmid name for ordering information...the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite Ling, et al...using the AAV44.9 serotype in future publications, please acknowledge John Chiorini and Shannon Boye and ...AAV44.9(E531D) serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...

7. Antibody Guide

   Type

   Collection

   ...code inserted into the plasmid. This produces the least amount of variation between antibodies, allows for... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...fragments - Fab fragments are created by enzymatically cleaving the variable regions of an antibody (each Fab ...they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles...single cells. Includes: Flow cytometry Read on to learn more about the applications common to each category...should be run with your protein of interest and at least one ubiquitously expressed protein such as GAPDH...protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced and amplified...

8. Genomic Deletions in Mammalian Cell Lines

   Type

   Collection

   ...amplify. Typically use primers at least 100 bp from the predicted cleavage site to ensure detection would...322.2 KB If you have trouble loading this video, please try viewing on YouTube . Abstract The prokaryotic...lines. Protocol For referenced Tables and Figures, please access the manuscript PDF posted at the top of ...genomic matches or near-matches to reduce risk of cleavage away from target sites (off-target effects). Ensure...which are likely to cause frameshift mutations leading to nonsense mediated decay of the mRNA transcript...of primers upstream and downstream of the sgRNA cleavage sites (“deletion band”; Figures 1 - 2 ). In the...for scarring (small indels produced at the sgRNA cleavage site without the intended deletion). Use a pair...

9. Malate Dehydrogenase CUREs Community Collection

   Type

   Collection

   ...MDH). The MCC is a national group of educational leaders providing research opportunities to their students...MDH and, if indicated on the plasmid map, a TEV cleavage site is present between the 6xHis-tag and the ... region of MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available... CUREs Community (Link opens in a new window) — Learn how to get started with a CURE, explore MDH projects...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and...window) External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan...undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate...

10. Retrograde AAV viral preps

    Type

    Collection

    ...CaMKII-somBiPOLES-mCerulean CamKII Bidirectional Optogenetics Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn Bidirectional...DREADDs Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825...GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting 51083 AAV-EF1a-DIO-...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Ting 83899...GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting 100853 pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

11. Zhang Lab CRISPR Page

    Type

    Collection

    ...-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system to facilitate genome...nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved in defense against invading phages...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease along with the guide RNA. For most applications...gain-of-function screening. For more information, please visit the Addgene SAM library page . Targeted gene... directed repair-mediated Kras G12D mutations, leading to macroscopic tumors of adenocarcinoma pathology... DNA targeting specificity of RNA-guided Cas9 nucleases. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann...

12. Bacterial Expression Systems

    Type

    Collection

    ...detection in western blots, or removed using protease cleavage sites. Other protein tags and signal peptides ... for protein purification. Commonly used tags, cleavage sites, and signal peptides include: Epitope tags...GST Solubility tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, factor Xa, enterokinase, ...Citrine (acceptor) FRET Wolf Frommer 65617 pFLIP42 mCerulean (donor) Citrine (acceptor) FRET Wolf Frommer 65618... screen the activity of arrays of zinc finger nucleases. Addgene Blog Introduction to FRET Bimolecular...interest at high levels. Perhaps high expression leads to slow bacterial growth, or maybe you only want...activity Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence...

13. Cre-Lox and Other Site-Specific Recombinases

    Type

    Collection

    ... and the systemic effects of Cre toxicity. Leakiness Leaky expression, where the gene of interest is expressed...Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome... from a mycobacteriophage, are serine SSRs that cleave all four DNA strands at once. They can mediate ...when a recombinase enzyme is present. Read on to learn about the different ways that Cre-lox and other ... Expression may cause repetitive recombination leading to genomic instability and cell death. It is important...populations. Read our blog post about how to minimize leakiness in experiments using AAV vectors. Highlighted ...

14. COVID-19 Resources

    Type

    Collection

    ...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...entry into cells. ( 2 ) FURIN - an enzyme that cleaves precursor proteins to a biologically active state... The SARS-CoV-2 S protein contains a potential cleavage site for furin proteases.( 3 ) BSG - (CD147), ...function.( 5 ) TACE - (ADAM17) a metalloprotease that cleaves hACE2, potentially increasing viral infectivity...degradation to develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth...et al, 2008, J Virol. Cathepsin L Functionally Cleaves the Severe Acute Respiratory Syndrome Coronavirus...Heurich A, et al , 2014, J Virol. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2...

15. CRISPR References and Information

    Type

    Collection

    ...analyze outcomes from cleaving nucleases (e.g. Cas9, Cas12a) and noncleaving nucleases (e.g. base editing...opens in a new window) A webtool that uses deep learning models for SpCas9 gRNA design and efficiency prediction...opens in a new window) A webtool that uses deep learning models for base editor gRNA design and efficiency...opens in a new window) A webtool that uses deep learning models for prime editor pegRNA design and efficiency...

16. Rett Syndrome

    Type

    Collection

    ...protein function results in Rett syndrome is not clear, however, the ability to bind to methylated DNA ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic...receptor (NCoR/SMRT) interacting domain (NID) a nuclear localization signal (not essential for localization...RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines are...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page...precise editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...your plasmids! To get started with your deposit, please click here ....

17. Optogenetics AAV Preps

    Type

    Collection

    ... 154948 CaMKII-somBiPOLES-mCerulean CamKII BiPOLES (soma-targeted) mCerulean Constitutive 5, 9, rg* Wiegert...154951 hSyn-DIO-somBiPOLES-mCerulean Syn BiPOLES (soma-targeted) mCerulean Cre dependent 5, 9, rg* Wiegert...Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

18. New England Biolabs Cell-Imaging Plasmid Collection

    Type

    Collection

    ...are also available Non-toxic to living cells To learn more about this system, visit the New England Biolabs...website. For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid...ACP-MCP-Tag plasmids - Substrate source (PDF, 46 KB) Please note: New England Biolabs (NEB) has discontinued...

19. Fluorescent Protein Guide: Biosensors

    Type

    Collection

    ...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted fluorescent...sensor (MARIO) A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically Encoded...3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad...sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin...

20. Mammalian RNAi Tools

    Type

    Collection

    ...Dicer, an RNase III enzyme, binds the shRNA and cleaves it to produce siRNA. The siRNA then binds the RNA-induced... siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute ...argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows a similar mechanism...Since it is pre-processed, it can bypass the Dicer cleavage step and proceed to binding of RISC. For more ...containing other RNAi components, like argonaute, please search our full site . ID Plasmid Vector Type PI...