We narrowed to 1,016 results for: LEA

...Expression mCerulean 433 475 27 4.7 Monomer (A206K) Cerulean - Mammalian Expression pCS2+8CCerulean - Zebrafish...pCS2+8NCerulean - Zebrafish/Xenopus/Worm/Sea urchin mCerulean N1 - Mammalian Expression mCerulean C1 -...Expression mCerulean3 433 475 32 3.2 Monomer (A206K) mCerulean3-N1 - Mammalian Expression mCerulean3-C1 - Mammalian... Mammalian Expression moxCerulean3 434 474 36 3.2 Monomer (A206K) moxCerulean3 - Mammalian Expression ...different colors as needed for your experiments. Learn more about the flexibility of self-labeling tags...

...targets may not result in equivalent cleavage efficiency. In fact, cleavage efficiency may increase or decrease...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...decrease expression of a target gene? Once you have a clear understanding of your experimental goal, you are...also important to consider factors that maximize cleavage of the desired target sequence, or on-target activity...exhibits the lowest immunogenicity and are one of the least toxic methods for in vivo viral delivery. Additionally...Before proceeding, we recommend asking labmates/colleagues, searching the literature, or discussing with...Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and gRNA. Before...

...a zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer...sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components of the...these tools and the researchers who are learning to wield them. Learn more about Addgene’s TAL effector and...years of research has shown that when fused to a nuclease or activation domain, a ZF array is an effective...at Massachusetts General Hospital also recently released a TALEN kit through Addgene. This kit, first described...

...available in each of the above serotypes and buffers. Please click on the plasmid name for ordering information...the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite Ling, et al...using the AAV44.9 serotype in future publications, please acknowledge John Chiorini and Shannon Boye and ...AAV44.9(E531D) serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...

...code inserted into the plasmid. This produces the least amount of variation between antibodies, allows for... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...fragments - Fab fragments are created by enzymatically cleaving the variable regions of an antibody (each Fab ...they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles...single cells. Includes: Flow cytometry Read on to learn more about the applications common to each category...should be run with your protein of interest and at least one ubiquitously expressed protein such as GAPDH...protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced and amplified...

...pmEYFP-1 , pECFP18aaEYFP mCerulean mVenus 433 0.49 527 104,000 0.64 5.3 2.3 mCerulean N1 , mVenus N1 mTurquoise2...Koushik et al. (2006) Cerulean, Venus, and VenusY67C FRET reference standards mCerulean-mVenus FRET efficiency...referred to as intramolecular or unimolecular FRET). Learn more about FRET on our blog and explore the tables...proteins connected via flexible peptide linkers mCerulean, mVenus, and Amber (mVenus Y67C) FRET standards...its use for measuring resonance energy transfer mCerulean, mVenus, and Amber (mVenus Y67C) constructs for...and Fixed Cells Additional Resources at Addgene Learn more on the Addgene Blog: Introduction to FRET and...

...MDH). The MCC is a national group of educational leaders providing research opportunities to their students...MDH and, if indicated on the plasmid map, a TEV cleavage site is present between the 6xHis-tag and the ... region of MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available... CUREs Community (Link opens in a new window) — Learn how to get started with a CURE, explore MDH projects...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and...window) External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan...undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate...

...CaMKII-somBiPOLES-mCerulean CamKII Bidirectional Optogenetics Simon Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn...DREADDs Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825...GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Jonathan Ting 51083 AAV-...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Jonathan ...GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Jonathan Ting 100853 pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system to facilitate genome...nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved in defense against invading phages...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease along with the guide RNA. For most applications...gain-of-function screening. For more information, please visit the Addgene SAM library page . Targeted gene... directed repair-mediated Kras G12D mutations, leading to macroscopic tumors of adenocarcinoma pathology... DNA targeting specificity of RNA-guided Cas9 nucleases. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann...

...detection in western blots, or removed using protease cleavage sites. Other protein tags and signal peptides ... for protein purification. Commonly used tags, cleavage sites, and signal peptides include: Epitope tags...GST Solubility tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, factor Xa, enterokinase, ...Citrine (acceptor) FRET Wolf Frommer 65617 pFLIP42 mCerulean (donor) Citrine (acceptor) FRET Wolf Frommer 65618... screen the activity of arrays of zinc finger nucleases. Addgene Blog Introduction to FRET Bimolecular...interest at high levels. Perhaps high expression leads to slow bacterial growth, or maybe you only want...activity Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence...

... and the systemic effects of Cre toxicity. Leakiness Leaky expression, where the gene of interest is expressed...Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome... from a mycobacteriophage, are serine SSRs that cleave all four DNA strands at once. They can mediate ...when a recombinase enzyme is present. Read on to learn about the different ways that Cre-lox and other ... Expression may cause repetitive recombination leading to genomic instability and cell death. It is important...populations. Read our blog post about how to minimize leakiness in experiments using AAV vectors. Highlighted ...

...analyze outcomes from cleaving nucleases (e.g. Cas9, Cas12a) and noncleaving nucleases (e.g. base editing...opens in a new window) A webtool that uses deep learning models for SpCas9 gRNA design and efficiency prediction...opens in a new window) A webtool that uses deep learning models for base editor gRNA design and efficiency...opens in a new window) A webtool that uses deep learning models for prime editor pegRNA design and efficiency...

...protein function results in Rett syndrome is not clear, however, the ability to bind to methylated DNA ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic...receptor (NCoR/SMRT) interacting domain (NID) a nuclear localization signal (not essential for localization...RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines are...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page...precise editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...your plasmids! To get started with your deposit, please click here ....

...are also available Non-toxic to living cells To learn more about this system, visit the New England Biolabs...website. For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid...ACP-MCP-Tag plasmids - Substrate source (PDF, 46 KB) Please note: New England Biolabs (NEB) has discontinued...

... 154948 CaMKII-somBiPOLES-mCerulean CamKII BiPOLES (soma-targeted) mCerulean Constitutive 5, 9, rg* Simon...154951 hSyn-DIO-somBiPOLES-mCerulean Syn BiPOLES (soma-targeted) mCerulean Cre dependent 5, 9, rg* Simon...Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted fluorescent...sensor (MARIO) A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...Nagai Magnesium MagFRET-1 Mg 2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically Encoded...3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad...sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin...

... ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...et al., 2020). FURIN plasmids - an enzyme that cleaves precursor proteins to a biologically active state... The SARS-CoV-2 S protein contains a potential cleavage site for furin proteases (Anderson et al., 2020...TACE plasmids - (ADAM17) a metalloprotease that cleaves hACE2, potentially increasing viral infectivity...Rottier, P. J. (2008). Cathepsin L functionally cleaves the severe acute respiratory syndrome coronavirus..., O., & Pöhlmann, S. (2014). TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2...

...Disease. These diseases persist for many years and lead to a major burden on the patients, their families.... Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using...spinal cord. Over time, a loss of motor neurons leads to limb weakness, difficulty speaking, swallowing...percentage (5–10%) are familial ALS cases having at least one other affected family member. These familial... individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It...libraries . Use the ultracompact and programmable endonuclease, TnpBmax , for genome editing. (Marquart et ...

...downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed...DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically...enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This... CRISPR plasmids designed for RNA targeting. To learn more about RNA targeting and other CRISPR topics...

...Dicer, an RNase III enzyme, binds the shRNA and cleaves it to produce siRNA. The siRNA then binds the RNA-induced... siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute ...argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows a similar mechanism...Since it is pre-processed, it can bypass the Dicer cleavage step and proceed to binding of RISC. For more ...containing other RNAi components, like argonaute, please search our full site . ID Plasmid Vector Type PI...