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We narrowed to 480 results for: Lif

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Showing: 21 - 40 of 480 results

  1. CRISPR Guide

    Type
    Collection
    ...experiments and must be amplified before use (Figure 8C), following the amplification protocol specified by...timing of CRISPR/Cas9 delivery. eLife . 3 e04766. https://doi.org/10.7554/eLife.04766 PMID: 25497837 Ma, Y.... by the depositing lab. After amplification, you’ll need to use next-generation sequencing (NGS) to verify...verify that the composition of your amplified library matches that of the original library. Next, you’... copies of the aptamer improves stability and amplifies the signal for better imaging of genomic loci,...SHERLOCK (and SHERLOCKv2) allows for greater amplification and quantification and can be used on lateral... Innovative Genomics Institute, University of California, Berkeley. Plan Your CRISPR Experiment Resources...

  2. mTOR Pathway

    Type
    Collection
    ... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...

  3. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    .... Subsequent freeze and thaw cycles reduce shelf life. To recover bacteria from your glycerol stock, open...from thawing completely and will improve the shelf life. It is very important that you shake the glycerol...

  4. Fluorescent Proteins: FRET

    Type
    Collection
    ... the acceptor. Alternatively, the fluorescence lifetime (the time between excitation of the fluorophore...of the donor can be measured using fluorescence lifetime imaging microscopy (FLIM) or FLIM-FRET, where ...energy transfer through FRET reduces the donor lifetime. FRET is often used to study 1) protein-protein.... https://www.leica-microsystems.com/science-lab/life-science/what-is-fret-with-flim/ (Link opens in a...

  5. Ligation Independent Cloning

    Type
    Protocol
    ...sterile dH20 to 40 μl Step 4: Amplify Insert by PCR Perform PCR amplification of your insert following the...

  7. Antibody Guide

    Type
    Collection
    ...Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification Methods ...present on the Fc region. This allows for the amplification of signals by having multiple secondary antibodies...on the antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific ...region of primary antibodies allows for signal amplification through the indirect detection method. In the...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...

  8. AAV Packaged on Request

    Type
    Collection
    ...preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such...legal approval and an implementation letter DNA amplification Viral vector production Density centrifugation... Quality is our priority. During this time, we amplify DNA, produce the prep, and perform comprehensive...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ... LSH-R LIF leukemia inhibitory factor (cholinergic differentiation factor) CDF, DIA, HILDA LIFR leukemia...factor receptor alpha CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS LRSAM1 leucine rich repeat and... GIF, GLIF, MMIF MLN motilin MGC138519 MLNR motilin receptor GPR38, MTLR1 MPL myeloproliferative leukemia... function as signaling proteins involved in proliferation, cell differentiation, and apoptosis. Plasmid...ACTH, CLIP, LPH, MSH, NPP, POC PPARA peroxisome proliferator-activated receptor alpha MGC2237, MGC2452, NR1C1... NR1C1, PPAR, hPPAR PPARD peroxisome proliferator-activated receptor delta FAAR, MGC3931, NR1C2, NUC1,...NUCI, NUCII, PPAR-beta, PPARB PPARG peroxisome proliferator-activated receptor gamma CIMT1, NR1C3, PPARG1...

  10. CRISPR References and Information

    Type
    Collection
    ...selection of organisms. From the Database Center for Life Science (DBCLS) in Japan. Developed by the Kumiko...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene ...CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system...

  11. Tetracycline Inducible Expression

    Type
    Collection
    ...transcriptional and proliferative state of each cell in a population. Proliferation history is monitored... tissue distribution, low toxicity, a known half-life (24 hours), and is relatively inexpensive. The induction...

  12. Cell Migration Consortium Plasmids

    Type
    Collection
    ...University of Virginia Denise Montell , University of California, Santa Barbara Martin Schwartz , Yale University...University of Illinois Ron Vale , University of California, San Francisco Browse Plasmids ID Plasmid Gene...

  14. Distribution to Industry

    Type
    Collection
    ...portion of Addgene's collection is available to qualified for-profit entities. Addgene reserves the right...right to determine if an entity is qualified to receive materials from our repository. All purchases are...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pHR-FUSN-mCh-Cry2WT FUS SFFV ALS Clifford Brangwynne 101224 pHR-FUSN-mCh-Cry2olig FUS SFFV ALS Clifford Brangwynne 101226...SFFV ALS Clifford Brangwynne 122441 pHR-FUSN-miRFP670-Cry2WT FUS Cry2WT, miRFP670 SFFV ALS Clifford Brangwynne...ALS2 ALS2 T7 Primary lateral sclerosis juvenile Cliff Tabin 19457 pcDNA5/FRT/TO HSPA1L HSPA1L CMV Parkinson's...101226 pHR-HNRNPA1C-mCh-Cry2WT HNRNPA1 SFFV ALS Clifford Brangwynne 101792 pMXs-mPKR-FLAG-IG EIF2AK2 Flag...HNRNPA1C::mCherry::SspB HNRNPA1 mCherry SFFV ALS Clifford Brangwynne 122669 M31: pHR-SFFVp- TDP-43C::mCherry...mCherry::SspB TARDBP mCherry SFFV ALS Clifford Brangwynne 123600 pcDNA6-N-3XFLAG-Vcp VCP Flag ALS Bruce...HNRNPA1-miRFP670-TRF1 HNRNPA1 miRFP670 SFFV ALS Clifford Brangwynne 228034 pDONR223 Rab25-STOP RAB25 T7...

  16. Open Enzyme Collection

    Type
    Collection
    ...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...

  17. AAV Molecular Tools

    Type
    Collection
    ...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru 99121 pAAV-ihSyn1...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru 117383 TRE-DIO-eYFP...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ...more likely a combination of gene variation and lifestyle or environmental factors. For example, variations...2019) pLVX-UbC-rtTA-Ngn2:2A:Ascl1 (Herdy et al. Elife. 2019) Use plasmids expressing SOX10, OLIG2, and...

  19. Plasmids for Stem Cell Research

    Type
    Collection
    ... Stem Cell Research Plasmids Stem cells are proliferative, unspecialized cells with the capacity to differentiate...optimize the conversion of fibroblasts into neurons. Elife. 2019 May 17;8. pii: 41356. Gage Fibroblasts iPSCs...

  20. Brain Initiative Collection

    Type
    Collection
    ...promoter that contains a positive feedback loop for amplifed expression of the tTA Viviana Gradinaru 99121-...promoter. Contains a positive feedback loop for amplifed expression of the tTA Viviana Gradinaru 99130-...
Showing: 21 - 40 of 480 results