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We narrowed to 526 results for: MEA;

Showing: 21 - 40 of 526 results

  2. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...examples of reporter plasmids that can be used for measuring infectivity and neutralizing activity are listed...

  5. AAV for Neuronal Tracing

    Type
    Collection
    ...from this cell to synaptically connected cells (meaning, it can spread to cells that are projecting to ...

  6. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Many viral plasmids are also self-inactivating, meaning that they can only complete one round of transcription...

  7. Microbiology Resources

    Type
    Collection
    ...Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus (WNV) Zika Don’t see your field...

  8. COVID-19 Resources

    Type
    Collection
    ...fluorescent reporter plasmids that have been used for measuring viral entry and activity in neutralization assays...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...cardiomyocytes using calcium activity as a functional measure of success. Mol Cell Cardiol. 2013 Apr 13;60C:97...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...acceptor fluorophore can be measured using microscopy techniques. FRET measurement sensitivity makes it suitable...and is the most rigorous method for measuring FRET. FLIM measures the fluorescence decay time of the donor...one of the first techniques which enabled the measurement of single molecule interactions in living cells...molecular interactions were detected by indirect means often using probes with the potential to target ...donor) to another fluorophore (the acceptor) by means of intermolecular long-range dipole-dipole coupling...living cells. Since then FRET has also been used to measure conformational changes, cleavage activity using...is still one of the best and most used pairs to measure FRET. The table below lists plasmids that can be...

  15. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...standard deviation of each replicate measurement divided by the mean of all the replicates multiplied by...allergy labs use standard curves with ELISAs to measure allergen concentrations in food; and your friend...dilute a control sample of known concentration and measure a specific response as a function of the concentration...the BCA reagent. The absorbance of both is then measured on a spectrophotometer and concentration versus...free of any contaminants that could affect the measurements. You'll need to prepare the standard, standard...curve? To create a standard curve, you graph the measured response of the serially diluted standard as a...concentration of the unknown sample based on its measured response. An example of this is shown in Figure...

  16. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...photostability of different fluorescent proteins? Measuring photostability Since it is clear that a photostable...photostability, an experiment is performed that measures fluorescence intensity over time. In order to ...with low excitation power. By repeating these measurements for different fluorescent proteins and by comparing...directly compared. An example of a photostability measurement of various cyan fluorescent proteins is depicted...protein distribution. Therefore, photostability measurements can be performed with soluble fluorescent proteins.... It should be noted that the photostability measurements reported in the literature are performed in ... the experimental designs used to make these measurements and hope this information can help guide your...

  17. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...  Intracellular protein targets, measured by fixing and permeabilizing membranes For intracellular protein...It does not, however, permeabilize cells and requires subsequent permeabilization with detergents such ...Have you ever wanted to measure expression of your protein of interest in a single cell? Or perhaps, ...is used for a variety of applications such as measuring target protein expression levels, assessing post-translational...flow cytometers detect Cell size and complexity, measured using visible light scatter The optical system...membranes produces a greater SSC.  Protein expression, measured using fluorescent protein fusions In addition ...uses 2 or 3 different fluorescent colors each measuring a different target. Flow cytometers however are...

  18. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...physical titer are direct p24 measurements via ELISA and qPCR for viral RNA. Measuring p24 levels in a viral ...particles. Direct measurement of lentiviral RNA is one alternative to direct p24 measurement. Like a p24 ELISA... as it only measures infectious viral particles. One of the si mplest methods of measuring functional ...flavors, physical or functional. Physical titers measure the amount of viral particles in a sample and are...acid. Functional titers, or infectious titers, measure how many of the viral particles produced can actually...drawback of this method, however, is that a p24 ELISA measures all p24 in the sample regardless of whether or...considered. Similar to the p24 assay, titering via measurement of viral RNA can overestimate the amount of infectious...

  19. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...donor/acceptor fluorophores (Stryer, 1978), a measurement that is unaffected by intervening proteins, lipid...HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim...blog has an excellent primer on FRET and how to measure it). Molecular mailbox: How to get your fluorophore... on the same street as the donor), then FRET measurements are possible. In fact, even if the majority ...properly targeted donor/acceptor pairs, then any measureable FRET will be swamped out by a sea of uncoupled...cell surface receptors) or with experiments on permeabilized cells. Biarsenical labeling reagents If you ...compounds cross cell membranes, thus enabling FRET measurements in intact cells. The Gradia Lab has deposited...

  20. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... ensure you are measuring the events-of-interest. Sensors suitable for ER measurements include: CEPIA ... requiring only a simple measurement. However, single wavelength measurements are more susceptible to ...subtype) and specific subcellular compartments for measurements. All of the sensors discussed below fall into...size doesn’t fit all, but never fear! That just means there is a great fit out there waiting for you. ...slower). GCaMPf may be suitable for very rapid measurements, like action potentials in neurons. jGCaMP7 ...additional versions are available to simultaneously measure calcium changes in the ER, cytosol and mitochondria...you combining your calcium reading with other measurements, specifically fluorescent ones? Many of the ...
Showing: 21 - 40 of 526 results