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We narrowed to 527 results for: MEA;

Showing: 21 - 40 of 527 results
  1. AAV for Neuronal Tracing

    Type
    Collection
    ...from this cell to synaptically connected cells (meaning, it can spread to cells that are projecting to ...
  2. Viral Vector Guides and Plasmids

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    Collection
    ...Many viral plasmids are also self-inactivating, meaning that they can only complete one round of transcription...
  3. Microbiology Resources

    Type
    Collection
    ...Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus (WNV) Zika Don’t see your field...
  4. COVID-19 Resources

    Type
    Collection
    ...fluorescent reporter plasmids that have been used for measuring viral entry and activity in neutralization assays...
  5. Plasmids for Stem Cell Research

    Type
    Collection
    ...cardiomyocytes using calcium activity as a functional measure of success. Mol Cell Cardiol. 2013 Apr 13;60C:97...
  6. Genetic Code Expansion

    Type
    Collection
    ... the aaRS enzyme form an orthogonal pair, which means they work independently of the host cell's natural...
  7. Antibody Guide

    Type
    Guide
    ... two types of antigen specificity: monoclonal, meaning many copies of one clone that are specific to a... a certain epitope, or polyclonal, meaning there are many clones that are specific to multiple epitopes...bound. Signaling molecules , proteins that emit a measurable light or color in response to a specific stimulus...proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here, HRP is used as ...samples can be normalized to a total protein measurement. Both methods will also serve as a control for...conjugate, typically HRP, is activated and its output measured. This method can be performed using the indirect...Fix your samples. Prepare your samples using permeabilization, antigen retrieval, and/or sectioning. Block...
  8. CRISPR Guide

    Type
    Guide
    ...based on the gRNA sequence without cleavage. This means proteins with other functions can be fused to a ...Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes they will be referred...principle, the ability to induce base transversions means that any base-to-base conversion is possible with...CASTs can result in bidirectional insertions — meaning they may not be suitable for applications where...Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall...
  9. Science Guides

    Type
    Guide
    ...Optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons) and their...optogenetics research utilize light to detect, measure, and control molecular signals and cells in order...
  10. Adenovirus Guide

    Type
    Guide
    ...and therefore needs to be supplied through other means. In order to produce rAdV vectors, E1 needs to be...Different serotypes exhibit varying tissue tropisms, meaning they preferentially infect certain tissues, which...
  11. Lentiviral Vector Guide

    Type
    Guide
    ...only infect mitotically active cell types. This means that lentiviruses can infect a greater variety of...populations that can be difficult to transduce by other means. A concern with gene therapies is the possibility...
  12. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAV serotypes exhibit strong neuronal tropism, meaning they can efficiently target specific brain regions...tools developed by researchers are used to detect, measure, and control molecular signals of cells (commonly...
  13. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...acceptor fluorophore can be measured using microscopy techniques. FRET measurement sensitivity makes it suitable...and is the most rigorous method for measuring FRET. FLIM measures the fluorescence decay time of the donor...one of the first techniques which enabled the measurement of single molecule interactions in living cells...molecular interactions were detected by indirect means often using probes with the potential to target ...donor) to another fluorophore (the acceptor) by means of intermolecular long-range dipole-dipole coupling...living cells. Since then FRET has also been used to measure conformational changes, cleavage activity using...is still one of the best and most used pairs to measure FRET. The table below lists plasmids that can be...
  14. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...standard deviation of each replicate measurement divided by the mean of all the replicates multiplied by...allergy labs use standard curves with ELISAs to measure allergen concentrations in food; and your friend...dilute a control sample of known concentration and measure a specific response as a function of the concentration...the BCA reagent. The absorbance of both is then measured on a spectrophotometer and concentration versus...free of any contaminants that could affect the measurements. You'll need to prepare the standard, standard...curve? To create a standard curve, you graph the measured response of the serially diluted standard as a...concentration of the unknown sample based on its measured response. An example of this is shown in Figure...
Showing: 21 - 40 of 527 results