...website contains additional information CRISPR Optimal Target Finder Identifies gRNA target sequences ...below: ID Plasmid 46294 pBS-Hsp70-Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila...hsp70 promoter. 45945 pHsp70-Cas9 : The codon-optimized Cas9 nuclease under the control of the Drosophila...

...intensity is 50% of its maximum value. Maturation is the time for fluorescence to obtain half-maximal value. Structure...Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation (nm) Emission (nm) Brightness... - Mammalian Expression Jump to Top Fluorescent Timers Protein Excitation (nm) Emission (nm) Brightness...with a wide variety of colors, some specifically optimized for yeast Lindquist Lab Vectors - Gateway cloning...

...mutant to inhibit MMR PEmax - optimized PE2 enzyme containing RT optimized for human codons, additional...PE2–PE5 systems PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition of RNA-binding...

...tag pAquaN1 Cyan Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express...pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet...pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet...

...Link opens in a new window) designed and codon-optimized the Open Enzymes parts for expression in Escherichia...pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB...pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI...

...generated using standard methods that have been optimized for each specific virus in order to generate high...Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

...of recoded E.coli (rEcoli XpS 192872 ) and an optimized phosphoserine orthogonal translation system (pSerOTS-C1... recoded strain of E. coli ( 68306 ) with the optimized phosphoserine orthogonal translation system ( ... and a new pSerOTS described in: System-wide optimization of an orthogonal translation system with enhanced...

... TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector...Sangamo)-Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled...replacing pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...

...promoters had been identified that responded to stimuli, such as hormones or metal ions; however, these...pursue a non-endogenous system for eukaryotes. At the time, bacterial systems from E. coli presented the best...Biotechniques . 2004 Oct;37(4):546, 548, 550. PubMed . Optimization of the Tet-On system for regulated gene expression...

...characterized by the progressive degeneration and ultimate death of neurons. Common neurodegenerative diseases...Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive...motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to limb weakness,...

...SARS-CoV materials are available only in the US at this time. Return to top Plasmids Encoding Mammalian Genes...RNAse activity of the Cas13 protein to develop and optimize the method termed Specific High Sensitivity Enzymatic...Libraries of Spike (S) Ectodomain and RBD mutants (Timothy Whitehead). SARS-CoV-2 Spike Receptor Binding Domain...

...tool for CRISPR/Cas design Program for designing optimal gRNAs. Provides feedback on number of potential...PAMs. Currently supports: mouse and human. CRISPR Optimal Target Finder Tool from the O'Connor-Giles Lab ...vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9 PDF 66.8 KB Fujii gRNA design and cloning...

... for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...

...infectious particle production, including codon optimization, deletion of the C-terminal 18-21 amino acids...SARS-CoV materials are available only in the US at this time. Accessory Plasmids Envelope and Packaging Plasmids...

...growth rate, so my cell pool is going to evolve over time, which is bad news for repeatability and bad news...had never done it before and it worked the first time probably because I used a robust cell line. If your...The Surveyor kit had a lot of good tips for first time users, all of which I followed. If you have never...the duplex and run it as an undigested control. Sometimes the PCR products can look smeared, so having an...products (GFP-GFP duplex) as a control. 4. After optimizing the reaction and confirming the presence of the...landscape of research opportunities, it’s an exciting time to be in the lab. Based on the power of available...John Doench & David Root and are described in Optimized sgRNA design to maximize activity and minimize...

...but are difficult to generate due to expense and time. An alternative approach is to use synthetically...include consistency between lots and the ability to optimize the antibody’s antigen binding sequence to improve...

...constructs in precise cell types and/or developmental timeframes. Here we highlight some of the plasmids and tools...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with...

...Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized ...Siliques CRISPR-mediated Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference...

...demethylation by Tet1. These modifications persist over time and are potentially heritable in dividing cells....target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

...Ras can activate a variety of pathways, which ultimately turn on genes involved in cell growth, differentiation... 1 RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide...