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We narrowed to 939 results for: TIM

Showing: 21 - 40 of 939 results

  1. Zebrafish Plasmid Collection

    Type
    Collection
    ...embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding, and transparency...proteins to visualize and track clones of cells over time. Examples of useful tools for studying cells that...clonal origin in zebrafish can be found below: Optimized QF-binary expression system for use in zebrafish...vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live...

  2. CRISPR References and Information

    Type
    Collection
    ... uses an open-source algorithm to automate and optimize gRNA and HDR donor design. The current version...Link opens in a new window) Program for designing optimal gRNAs. Provides feedback on number of potential...human. Developed by the George Church Lab . CRISPR Optimal Target Finder (Link opens in a new window) Identification...vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...mutant to inhibit MMR PEmax — optimized PE2 enzyme containing RT optimized for human codons, additional...PE2–PE5 systems PE6 — small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding...

  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Photoactivatable Photoconvertible Photoswitchable Timers Self-Labeling Tags Additional Resources The following...drops to half its maximum value. Maturation is the time for fluorescence to reach half its maximal value... Mammalian Expression Return to top Fluorescent Timers Fluorescent protein variants that change their ...their fluorescence from the blue to red over time, allowing tracking of processes such as intracellular trafficking...

  5. Open Enzyme Collection

    Type
    Collection
    ...Link opens in a new window) designed and codon-optimized the Open Enzymes parts for expression in Escherichia...pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB...pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI...

  6. Viral Production

    Type
    Collection
    ...generated using standard methods that have been optimized for each specific virus in order to generate high...Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

  7. COVID-19 Resources

    Type
    Collection
    ...production, secretion, and codon-optimized versions designed for your expression system. ...SARS-CoV materials are available only in the US at this time. Return to top Plasmids Encoding Mammalian Genes...Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain and...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ... TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector...Sangamo)-Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled...replacing pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...

  9. Neurodegeneration Research Collection

    Type
    Collection
    ...characterized by the progressive degeneration and ultimate death of neurons. Common neurodegenerative diseases...Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive...motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to limb weakness,...

  10. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...rEcoli XpS (Bacterial strain #192872) , and an optimized phosphoserine orthogonal translation system, pSerOTS-C1... C321.ΔA (Bacterial strain #68306) , with the optimized phosphoserine orthogonal translation system, SepOTSλ...Rogulina, S., & Rinehart, J. (2023). System‐wide optimization of an orthogonal translation system with enhanced...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ... for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...

  12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...growth rate, so my cell pool is going to evolve over time, which is bad news for repeatability and bad news...had never done it before and it worked the first time probably because I used a robust cell line. If your...The Surveyor kit had a lot of good tips for first time users, all of which I followed. If you have never...the duplex and run it as an undigested control. Sometimes the PCR products can look smeared, so having an...products (GFP-GFP duplex) as a control. 4. After optimizing the reaction and confirming the presence of the...landscape of research opportunities, it’s an exciting time to be in the lab. Based on the power of available...John Doench & David Root and are described in Optimized sgRNA design to maximize activity and minimize...

  13. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...infectious particle production, including codon optimization, deletion of the C-terminal 18-21 amino acids...SARS-CoV materials are available only in the US at this time. Accessory Plasmids Envelope and Packaging Plasmids...

  14. Antibody Plasmid Collection

    Type
    Collection
    ...but are difficult to generate due to expense and time. An alternative approach is to use synthetically...include consistency between lots and the ability to optimize the antibody’s antigen binding sequence to improve...

  15. Worm Expression Resources

    Type
    Collection
    ...constructs in precise cell types and/or developmental timeframes. Here we highlight some of the plasmids and tools...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with...

  16. Plant Plasmids and Resources

    Type
    Collection
    ...Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized ...Siliques CRISPR-mediated Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference...

  17. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...demethylation by Tet1. These modifications persist over time and are potentially heritable in dividing cells....target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  18. Ras Pathway

    Type
    Collection
    ...Ras can activate a variety of pathways, which ultimately turn on genes involved in cell growth, differentiation... 1 RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...targeting : Cas13 enzymes target RNA rather than DNA, sometimes requiring a protospacer flanking sequence (PFS...imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . 155(7):1479-91. PMID...

  20. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...webinar! Special thanks to our panel of experts, Tim Miles, David Goertson, Xinhong Chen, and Miggy Chuapoco...didn’t get answered live - but he kindly took some time to address all your unanswered questions via text...Chuapoco, and to all our participants for their time and excellent questions.    Additional Resources...Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org Find Adenovirus...
Showing: 21 - 40 of 939 results