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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 318 results for: atic

Showing: 21 - 40 of 318 results

  1. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...opportunity to develop and use patient-specific somatic cells that are otherwise difficult to obtain. These...cell plasmids at Addgene Many sources of adult somatic cells have been probed for their suitability in...These advantages make PBMCs the ideal source of somatic cells for reprogramming purposes. In this post,...reprogramming often depends on the source of the somatic cells, the common goal of all reprogramming methods... only 0.001% when using PBMCs as the source of somatic cells. This translates to only about 16 hiPSC colonies... major hindrance in using PBMCs as a source of somatic cells for reprogramming. Enhancing PBMC reprogramming...

  2. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...bactericidal) or slow growth/prevent cell division (bacteriostatic); however, the distinction between the two ...categories may be a bit of a gray area as some bacteriostatic reagents can kill bacteria when used at high...subunit; inhibits aminoacyl translocation Bacteriostatic 50-100 ug/mL in EtOH Polymyxin B polypeptide...inhibits protein synthesis (elongation step) Bacteriostatic 10 ug/mL Chloramphenicol   Binds 50S ribosomal...ribosomal subunit; inhibits peptidyl translocation Bacteriostatic 5-25 ug/mL in EtOH *In prokaryotes.     ...

  3. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ... variants across a large number of genes as systematically as possible. In developing these assays, we...to demonstrate how gene expression profiling, systematic tumorigenesis assays, drug epistasis assays, ...genes: High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, Wu X, et al. Cancer...mutant collection is described in this paper. Systematic Functional Interrogation of Rare Cancer Variants...reference collections are described in this paper Systematic morphological profiling of human gene and allele...

  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...macromolecule. Biotin can be added enzymatically or chemically. Enzymatic biotinylation occurs naturally ...using a biotinylated primer. Biotin can also be enzymatically incorporated during PCR using a biotin labelled...sometimes easier and less expensive than other enzymatic methods. Scientists have commonly used this method...10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic biotinylation of proteins: A post-translational...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Indicators to Image Dynamic Zn(2+) Secretion of Pancreatic Islets. Anal Chem. 2019 Oct 1;91(19):12212-12219...One. 2014 Oct 23;9(10):e110600. Isei Tanida Electrostatic potential Quantitative measurements of membrane...

  6. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ... Patrick Kwok-Shing, et al. "Systematic functional annotation of somatic mutations in cancer." Cancer ...Plasmid Collection for “Functional Annotation of Somatic Mutations in Cancer.” Of all the mutations and ...annotation of rare gene aberration drivers of pancreatic cancer." Nature communications 7 (2016): 10500...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...sets and most machines have a protocol that automatically performs the normalization step.  Machines Most...and sets.   🔥Hot Tip🔥 The ABI machines automatically record fluorescent data on all channels - helpful... of PCR products in a rich web application, Bioinformatics, Volume 27, Issue 7, 1 April 2011, Pages 1019...1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman WM; Walker SJ; Vrana KE. 1999. Quantitative...Statistical analysis of real-time PCR data. BMC Bioinformatics 7, 85 (2006). https://doi.org/10.1186/1471-...

  8. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...donor [7]. Adult stem cells (ASCs) Also known as somatic stem cells, these multipotent stem cells are located...we’ll discuss the two widely used approaches of somatic cell conversion: conversion via iPSC formation,... reprogramming and, direct conversion from one somatic cell type to another, also known as transdifferentiation...pluripotent stem cell lines derived from human somatic cells. Science, 2007. 318(5858): p. 1917-20. PubMed...

  9. MXS Chaining

    Type
    Blog Post
    ...High-throughput cloning, in a nutshell, is the systematic combination of different genetic sequences into...L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of DNA molecules up to several hundred... 19363495. 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. VIII. Frequencies...PMC4409215. 7. Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XI. Further...

  10. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...conjugate with an enzymatic label such as horseradish peroxidase (HRP). Experiments using enzymatic labeling can...2019; McLaughlin, 2019).    Figure 1: Schematic of multiplex immunofluorescence approaches. A)...yourself stuck with red-green images generated automatically from the microscope software, you can always...

  14. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional...

  15. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...asking two questions: 1) Is the sample dynamic or static? 2) Is the sample thin (< 15 μm) or thick? These... effects of high light doses to the cells. Thin static samples Ex: Fixed monolayers of cells or thin (...microscopy can be combined with deconvolution, a mathematical software post-processing step that reassigns...in light penetration mentioned above (5). Thick static samples Ex: Fixed tissue sections (> 15 um), 3D...

  17. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654 and ...

  18. TALEN Engineering

    Type
    Collection
    ... ( Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells ( Miller et al., Nat Biotechnol. 2011 ; Reyon...

  19. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window, and are...
Showing: 21 - 40 of 318 results