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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 704 results for: des.1

Showing: 21 - 40 of 704 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6...SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide ...screens. The libraries are available in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together..., Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science . 2014 Jan 3;343(6166...):186-91. doi: 10.1038/nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids...oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed based on...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids...

  2. Lentivirus Plasmids

    Type
    Collection
    ...search "lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid...Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy...working with lentivirus: these viruses are based on HIV-1, which may require additional lab biosafety procedures... EGFP coexpression. Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression...plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible...lentiviral systems available at Addgene have been designed for increased researcher safety. Read our Lentiviral... are used in conjunction with the plasmid that encodes the viral genome (typically called the transfer...

  3. Lentiviral Prep Service

    Type
    Collection
    ...version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially...Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version 1 Ready-to-use lentiviral... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...Version 2 of the Celltag library contains 4934 barcodes to combinatorially index cells for single-cell...Version 3 of the Celltag library contains 5737 barcodes to combinatorially index cells for single-cell...

  4. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense), and four transposase components (TnsA, TnsB...some mammalian systems have been developed. Figure 1: Overview of the basic CAST mechanism. Created with...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated transposases...

  5. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... orientation of each target site (see Figure 1). Figure 1: Recombination outcomes are determined by target...minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and Flp Recognition Sites. Controlling...target site sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-, Flp-, or...efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector...light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...

  6. Mammalian RNAi Tools

    Type
    Collection
    ...more information on shRNA design and delivery, see resources below. Figure 1: Overview of shRNA-mediated...Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols...target sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute protein 2 (Ago2), which ...RISC). RISC unwinds the double-stranded siRNA and degrades one of the strands. The remaining single-stranded... Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab...Taxman, D. J. (2010). Short hairpin RNA (shRNA): design, delivery, and assessment of gene knockdown . Methods...

  7. Viral Vector Guides and Plasmids

    Type
    Collection
    ...integration. Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral plasmids...viral vector guides and plasmids available at Addgene and other related resources. Science... Science Guides Viral Vectors...Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors Resources...selection of in-stock viral preps available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral...the production of viral vectors that contain the desired genetic cargo. In turn, these viral vectors are...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...system. ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On...and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic tool to control...skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR...although the DNA-binding profile is reversed (Figure 1). However, rtTA can induce stronger expression of ...Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR from CMV...promoters (sometimes generally called Ptet) have been designed by combining tet O sequences with elements like...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...

  9. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Link opens in a new window) References Botman, D., de Groot, D. H., Schmidt, P., Goedhart, J., Teusink,...fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913-z ...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3 BbsI Injection or in vitro transcription... , Harrison , Wildonger 49408 pCFD1-dU6:1gRNA dU6:1 BbsI Injection or in vitro transcription Virmilion...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ... to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID ...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...

  11. Brain Initiative Collection

    Type
    Collection
    ...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV2 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV5 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV8 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV9 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-AAVrg pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-PHPeB pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

  12. Retrovirus Plasmids

    Type
    Collection
    ...10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for ...envelope and packaging plasmids. ID Plasmid Type Description PI 8449 pUMVC Packaging Contains MoMLV gag and...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...

  13. AAV for Neuronal Tracing

    Type
    Collection
    ...rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA and G-protein is delivered to the...with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932 Wickersham IR, Lyon DC, Barnard...Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...

  14. Antibody Plasmid Collection

    Type
    Collection
    ...phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression...immunostaining. Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated...create and express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy...for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link opens in a new...populations defined by multiple parameters. Figure 1: Examples of intersectional cell population definitions... as a proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach...interneurons encode fear memory. Nat Neurosci. 23(1):61-74. PubMed (Link opens in a new window) Hafner... experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...

  16. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Service at Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid, expressing...effects in vivo . While AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled... are used in conjunction with the plasmid that encodes the viral genome (typically called cis -plasmid...plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for AAV...

  17. Genetic Code Expansion

    Type
    Collection
    ...Bacterial TAG Farren Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial...Bacterial TAG Farren Isaacs 73547 pEvol-pAzFRS.1.t1 pAzFRS.1.t1 E. coli p-azido-l-phenylalanine (pAzF) Bacterial...7FTrp) Bacterial TAG Thomas Huber 207639 pEVOL-NBK-1 PylRS M. mazei Pyrrolysine Bacterial Cole DeForest...and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair instead. For...based upon four nucleotides, 64 codons, and 20 amino acids. Yet in the past two decades, biologists have...included. For any and all questions you have while designing, implementing, or troubleshooting GCE experiments...non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites changes to UAG...

  19. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...donor and acceptor. FRET is often used to study (1) protein-protein interactions where each protein is...standard consisting of ECFP and EYFP connected by 1 flexible glycine- and serine-containing peptide linker...fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine...

  20. Rett Syndrome

    Type
    Collection
    ...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development...in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene activation... the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...BrainSpan Atlas of the Developing Human Brain - includes developmental transcriptome information, prenatal...
Showing: 21 - 40 of 704 results