We narrowed to 718 results for: des.1

...integration. Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral plasmids...viral vector guides and plasmids available at Addgene and other related resources. Science... Science Guides Viral Vectors...Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors Resources...selection of in-stock viral preps available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral...the production of viral vectors that contain the desired genetic cargo. In turn, these viral vectors are...

... orientation of each target site (see Figure 1). Figure 1: Recombination outcomes are determined by target...minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and Flp Recognition Sites. Controlling...target site sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-, Flp-, or...efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector...light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...

...Link opens in a new window) References Botman, D., de Groot, D. H., Schmidt, P., Goedhart, J., Teusink,...fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913-z ...

...more information on shRNA design and delivery, see resources below. Figure 1: Overview of shRNA-mediated...Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols...target sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute protein 2 (Ago2), which ...RISC). RISC unwinds the double-stranded siRNA and degrades one of the strands. The remaining single-stranded... Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab...Taxman, D. J. (2010). Short hairpin RNA (shRNA): design, delivery, and assessment of gene knockdown . Methods...

...system. ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On...and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic tool to control...skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR...though the DNA-binding profile is reversed (Figure 1). However, rtTA can induce much stronger expression...Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR from CMV...promoters (sometimes generally called pTet) have been designed by combining tet O sequences with elements like...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...

...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV2 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV5 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV8 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV9 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-AAVrg pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-PHPeB pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

...rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA and G-protein is delivered to the...with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932 Wickersham IR, Lyon DC, Barnard...Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...

...gRNA expression . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid...Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy...coexpression. Didier Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression... Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible...Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression. Miguel Ramalho-Santos...Tet-inducible lentivirus for ORF expression, multicistronic design enables tracking of viral transduction. See plasmid... based) for inducible knockdown, multicistronic design enables tracking of viral transduction. See Meerbrey...

...phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression...immunostaining. Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated...create and express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy...for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions...

...Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link opens in a new...populations defined by multiple parameters. Figure 1: Examples of intersectional cell population definitions... as a proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach...interneurons encode fear memory. Nat Neurosci. 23(1):61-74. PubMed (Link opens in a new window) Hafner... experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...

...Bacterial TAG Farren Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial...Bacterial TAG Farren Isaacs 73547 pEvol-pAzFRS.1.t1 pAzFRS.1.t1 E. coli p-azido-l-phenylalanine (pAzF) Bacterial...recognized by the cognate orthogonal tRNA. Figure 1: Use of synthetase and target plasmids in genome code...7FTrp) Bacterial TAG Thomas Huber 207639 pEVOL-NBK-1 PylRS M. mazei Pyrrolysine Bacterial Cole DeForest...and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair instead. For...genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids. Scientists have...included. For any and all questions you have while designing, implementing, or troubleshooting GCE experiments...

...in the iNDI plasmid collection at Addgene. Figure 1: Schematic of iNDI iPSC line generation, phenotyping...types, as noted in the purpose field: ID Plasmid Description Additional Resources iNDI (Link opens in a new...

...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development...in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene activation... the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...BrainSpan Atlas of the Developing Human Brain - includes developmental transcriptome information, prenatal...

...small enough to be packaged in AAV particles. Figure 1: Overview of RNA targeting with CRISPR. Browse, sort...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...mutation reduces off-target effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-...or search the tables below for CRISPR plasmids designed for RNA editing. To learn more about RNA editing... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

..., or the beginning of the coding sequence. Figure 1: Overview of CRISPR interference. Browse, sort, or... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

... assembly reaction** 1 5 min., 37 ºC (2.5 μl) 687 100% 274,200 2,742,000 1 60 min., 37 ºC (2.5 μl)...expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed a mixture...representative transformation plates obtained from 1-, 12- and 24-fragment assemblies of the lacI/lacZ ...lacZ cassette, and illustrates how the volume of the 1 ml outgrowth spread on each transformation plate can...fidelity studies conducted by Potapov et al. at NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological...over 90% for 24-fragment assemblies (Figure 3, Table 1). These improvements (5- to 12-fold increases in the... 16 ºC) x 30 (100 μl) 78 90.7% 783 9,792 Table 1. Yields and fidelities for Golden Gate Assemblies ...

...start designing the specific components (Cas enzyme and guide RNA) for your experiment (Figure 1). You...gRNA), the design of the gRNA sequence, and finally how to validate your genetic edit. Figure 1: Flow chart...population. Some cells may remain wild type due to either (1) a lack of gRNA and/or Cas9 expression or (2) a lack...Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit, A., Parnas, O...used for gRNA design. Read more about how to design your gRNA . Synthesize and Clone Desired gRNAs Once ...multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down to location...on proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...

...also indirectly modify local chromatin state. Figure 1: Overview of epigenetic modification using CRISPR....or search the tables below for CRISPR plasmids designed for epigenetic modification. To learn more about... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of specific genomic regions...CRISPR plasmids that have been designed for use in bacteria. CRISPR...Resources The following CRISPR plasmids have been designed for use in bacteria. To find emtpy gRNA plasmids... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...the dCas9-activator to your specific locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...