We narrowed to 634 results for: des.2

...M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries, R., Brouns, S. J., & Van Der...is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental...Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview of the NHEJ repair mechanism. Multiplex ...systems enable researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single...Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...your experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with...the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar to Cas9 and Cas12...

...Layer 2-3_IT Isocortex 230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT ...Layer 2-3_IT Isocortex 220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT ...Layer 2-3_IT Isocortex 230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT ...populations using systemic AAV delivery. The collection includes the top vectors with the highest specificity and...

...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex, comprised of a combination of Cas5...are largely underutilized compared to their Class 2 counterparts like Cas9. This is attributed in part... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute protein 2 (Ago2), which facilitates... International Journal of Biomedical Science, 13 (2), 48–57. PubMed (Link opens in a new window) . Moore...RISC). RISC unwinds the double-stranded siRNA and degrades one of the strands. The remaining single-stranded... binding of RISC. For more information on shRNA design and delivery, see resources below. Figure 1: Overview... Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab...Taxman, D. J. (2010). Short hairpin RNA (shRNA): design, delivery, and assessment of gene knockdown . Methods...

...containing transgene under CMV Bamburg 16407 pAdEasy 2-GFP beta-gal Test plasmid that contains β-gal and ...use in AdEasy™ System Vogelstein 16401 pAdEasy-2 Adenoviral vector for use in AdEasy™ System, for use with...recombination with the shuttle plasmid. This collection provides a curated selection of popular plasmids that can...for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production of viruses containing...adenoviral vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use ... for adenoviral vector production. ID Strain Description PI 16398 BJ5183 E. coli strain for recombination...

...suggests that titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard...catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre... a new vial of early passage cells. Approximately 2 weeks post-thaw, cell culture supernatant is tested...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...

...phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression...+J phagemid libraries. Immunol Lett. 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for...create and express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy...for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions...

...constitutive Expression of the tet-off transactivator (tTA). 2 Viviana Gradinaru 99120 pAAV-ihSyn1-tTA Inducible ...receptor fused to GFP for studying cell ablation. 2 Thomas Jessell , Eiman Azim 207997 pAAV hSyn-fDIO-...Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation...

...components (Figure 2), and the optimal system will likely depend on your cell type. Figure 2: Different delivery...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that...CRISPR-Based genome editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006...used for gRNA design. Read more about how to design your gRNA . Synthesize and Clone Desired gRNAs Once ...multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down to location...on proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...

...2007 ) in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for expression of both a “+” and...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...Expression Vectors This page lists plasmid vectors designed to express nucleases in different cell types. ...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

...cells; function is unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible ...such as anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found...secondary immune response Monomer with a valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary... ranging from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include...used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared to alternative... is described in more detail in the Multiplex Assays section below. The following table describes the ... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody...

...196208 GB1-A11(2-196)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196209 GB1-A11(2-196, G38R)-Strep...196211 GB1-A11(2-196, G175R)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196212 GB1-A11(2-196, G189E...8661 p4455 FLAG-hPLIC-2 UBQLN2 Flag CMV ALS Peter Howley 8662 p4456 FLAG-hPLIC-2 NTF UBQLN2 Flag CMV ALS...Pietro De Camilli 194005 VPS13D1-1576-(Y1253S) VPS13D GFP CMV Spinocerebellar Ataxia Pietro De Camilli...Parkinsonism Pietro De Camilli 232865 mCherry-VAB(VPS13C) VPS13C mCherry CMV Parkinsonism Pietro De Camilli 232866...Parkinsonism Pietro De Camilli 232867 VPS13C^Halo-delta(ATG2C-PH) VPS13C Halo CMV Parkinsonism Pietro De Camilli ...Parkinsonism Pietro De Camilli 232869 mCherry-PH(VPS13C) VPS13C mCherry CMV Parkinsonism Pietro De Camilli 232872...

...AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde Retrograde AAV To ...comprehensive high-quality quality control process includes titering, purity assessment, sterility testing...

...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh...Protocols, 2016 . Yamamoto Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system has been...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information...information about HDR/repair template design. How to use CRISPR to tag your gene of interest Mendenhall and...interest is currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein...protein to your target sequence, as well as design and clone in the homology arms for the donor plasmid. The...

...optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a ...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for... "transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to...

...chromosomal integration of DNA. ACS Synthetic Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing...CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the ...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at...mcherry with either 0, 11, 18, or 20 matching nucleotides. Together, these plasmids allow to explore the...

... funding from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement No 777372....proteomic interactions represent the framework that provides biological context. Assays monitoring impedance...sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled...solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561 Superti-Furga...

...LuxSit-i and neoLux1.2 : Artificial luciferases desined de novo using deep-learning approaches. Split Luciferase...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion...Structurally destabilized firefly luciferases : A set of three increasingly destabilized mutants which...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion of...plasmids. ID Plasmid Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc...your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return to Top Other Highlighted...

...window) An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...CRISPR/Cas design (Link opens in a new window) Program for designing optimal gRNAs. Provides feedback on...window) This tool helps design (10 different prediction scores), clone (primer design), and evaluate gRNAs...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...window) CRISPR/Cas9 reagent generation; gRNA design; HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor...CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums. CRISPR...

...outgrowth volumes described in Figure 3, with calculated yields from entire outgrowth built from 2 µl of the ...Golden Gate Assembly is commonly used to assemble 2–10 DNA fragments in a single “one-pot” reaction to...the transformants onto the selection plate. Figure 2 shows representative transformation plates obtained...fidelity studies conducted by Potapov et al. at NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological... (percent correct) Calculated colony totals Per 2 μl assembly reaction Per full assembly reaction** ...fidelity and bias profiling." bioRxiv (2018): 322297. 2. Potapov, Vladimir, et al. "A single-molecule sequencing... push the test system, we redesigned for 12 and 24 fragments.  This redesign was guided by both advances...