We narrowed to 810 results for: mal

...(Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG Huiwang...AbK Mammalian TAG Simon Elsaesser 140011 pAS_4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG ...-aminotyrosine Mammalian Huiwang Ai 154762 pAS_FLAG-Mma PylRS PylRS M. mazei Mammalian TAG Simon Elsaesser...archaeon Mammalian TAG Simon Elsaesser 154764 pAS_FLAG-Mx1201 PylRS PylRS M. alvus Mammalian TAG Simon...acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS (human-opti) PylRS M. flavescens acetyl-lysine Mammalian Tao...mazei Mammalian TAG Simon Elsaesser 174896 pAS_4xMmaPylT(UUA)_EF1_MmaPylRS PylRS M. mazei Mammalian TAA ...mazei Mammalian TGA Simon Elsaesser 174901 pAS_MmaPylT_EF1_NES-MmaPylRS WT PylRS M. mazei Mammalian TAG ...

...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3) GTPase Arfrp1 10139 201 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap2 84364 521 Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap3...Arap3 (CENTD3) 64411 1544 Mammalian (pEGFP-C2) GAP Acap1 (CENTB1) 9744 740 Mammalian (pFLAG-CMV2) GAP Acap2...

...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression...or miniTurbo in the mammalian nucleus pSF3-ultraID - Expresses ultraID in mammalian cells with the Tet-...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins...proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible...FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian expression pNIC-CTHF - C-terminal ...including Xenopus HA Epitope tag pcDNA3.1-HA - Mammalian expression vector with N-terminal HA tag pInducer20...Myc Epitope tag pKMyc - N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector ...

... CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression...of organisms, ranging from bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation ...Gaussia luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase ...but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and ATP-independent, and ...noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s) Description...the control of a CMV promoter is present for normalization William Kaelin 71248 pXPG Firefly Insertion ... expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 212936 pGL3 Basic Vector...

...29;7:12266. David Savage Maltose Monitor maltose levels and metabolism (FLIPmal) Fluorescence Resonance...Calcium Small near-infrared indicator iGECInano Design and Initial Characterization of a Small Near-Infrared...:19562. Takashi Tsuboi Maltose Single fluorescent protein biosensor for maltose Rapid construction of ...Jun 3. 1(1):11. Wolf Frommer Maltose Green fluorescent MBP-based maltose biosensor A genetically encoded...Biosensors Fluorescent Proteins: Biosensors Metal Ions Small Molecules Neurotransmitters Cell Physiology Browse...Diverse Functional Payloads to Cells of Behaving Mammals. Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-...

...Browse Plasmids: Anti-CRISPR Small Precision Edits CRISPR can be used to make small, very precise edits of ... (dsDNA) plasmid. Small single-stranded DNA (ssDNA) oligos often work well for small edits like a single-point...Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion...missing sequence, and is more commonly used for small precision edits. NHEJ directly ligates the break...efficient in repairing DSBs, it frequently causes small nucleotide insertions or deletions (indels) at the...modification ). In most cases, NHEJ gives rise to small indels in the target DNA that result in amino acid... handful of bases, or even a single base. These small precision edits can be done via HDR, base editing...

...methods: in animals, in cultured immune cells, or through the use of recombinant plasmids. Animals - Antibodies...Antibodies produced in animals make use of the animal’s immune system. The antigen of interest is introduced...introduced to the animal, which then mounts an immune response to it. Once the immune response has developed...antibody. Monocultures of B cells isolated from animals immunized as described above are used to create... protein chains: two large heavy chains and two small light chains. These four domains come together to...only produce one variable region. Isotypes The mammalian immune system produces five classes of antibodies...antibodies are typically collected directly from animals and contain antibodies targeting various epitopes...

...19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the TetR protein binds to tet O, blocking...typically features seven copies of tet O upstream of a minimal CMV promoter, and other tet- or dox-dependent promoters... placing seven copies of tet O upstream of the minimal CMV promoter. In the absence of tetracycline, tTA...sensitivity to doxycycline and three copies of a minimal activation domain from VP16 for increased induction...engineered to eliminate binding of endogenous mammalian transcription factors to further minimize background... expression of the transactivator proteins in mammalian cells or other species by codon optimization of...separate vectors. Stable cell lines or transgenic animals can be made to achieve greater control over expression...

...GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian hU6 epegRNA...CRISPR Prime Edit CRISPR Plasmids: Prime Edit Mammalian Bacteria Drosophila Plant Empty pegRNA Resources... activity; can be used in PE2–PE5 systems PE6 — small prime editors with optimized RT and/or Cas9 domains... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6 pegRNA BsaI No mRFP1 David Liu 140448 QPM-... No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI...sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob ...

...-Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled...destination vectors and a reporter vector for mammalian cell-based validation assay that are designed ...vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing unique... contains the VP64 domain (four repeats of the minimal activation domain of VP16) at the C terminus. 47389...Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize...plasmids were optimized for TALEN expression in mammalian systems including hard-to-transfect cell lines...sequence to drive efficient TALEN expression in mammalian cells, (iv) an improved, truncated TALE backbone...

...vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with smaller Cas enzymes...focus on making single edits using CRISPR/Cas9 in mammalian cells as an example, but many of these principles...common categories of genetic manipulations used in mammalian cells and their corresponding Cas enzymes. In ...specific genomic edits, like point mutations or small tag insertions, by copying from a DNA donor template...exert their activity within a very narrow window proximal to the PAM sequence Base Edit Prime Edit Generate...KRAB can be more effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase... and base editor variants often have different optimal editing windows. Be sure to refer to the literature...

...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development...Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources...criteria, defined as the presence of relatively normal early development, regression with a loss of spoken... rare cases Rett syndrome can be inherited. In females, random X inactivation results in approximately...PMID: 21074045 2017 - the Bird lab determines a minimally functional MECP2 that rescues neurological defects...opens in a new window) PMID: 29019980 Return to top Animal Models Studies of the MECP2 protein in mouse and...general introduction to CRISPR technology or the mammalian CRISPR resources for a full selection of plasmids...

... nuclease domains: pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in... domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802 (for expression of...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette...domain ( Sollu et al., Nucleic Acids Res ) in mammalian cells or zebrafish). pCLR2068 (for expression ...domain. ( Doyon et al., Nat Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al...

...2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell culture. Five ... specialized Cas proteins snip foreign DNA into small fragments approximately 20 bp in length and paste...characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9... improving targeting in AT-rich genomes. Cpf1's small size also makes it suitable for multiplexing and...permanent genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific... this non-specific cleavage does not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also ...has CRISPR plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. ...

...sources of antibodies for research purposes include animals or hybridoma cells, but are difficult to generate...into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits...recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived from ...the plasmids are transiently transfected into mammalian tissue culture cells, the R-mAbs are secreted ...use. Other scientists may choose to work with a smaller fragment of an antibody, which could offer increased...increased stability and ease of production due to their small size. These recombinant antibody fragments can be...antibody heavy- and light-chain DNAs into a single mammalian expression vector A tool kit for rapid cloning...

...DHLAG, HLADG, Ia-GAMMA CD8A CD8a molecule CD8, Leu2, MAL, p32 CD8B CD8b molecule CD8B1, LYT3, Leu2, Ly3, MGC119115...cytotoxicity triggering receptor 3 1C7, CD337, LY117, MALS, NKp30 NFAT5 nuclear factor of activated T-cells...TRAP, gp39, hCD40L CD8A CD8a molecule CD8, Leu2, MAL, p32 CD8B CD8b molecule CD8B1, LYT3, Leu2, Ly3, MGC119115... HSCR, HSCR2 EGF epidermal growth factor (beta-urogastrone) HOMG4, URG EGFR epidermal growth factor receptor...Categories Plasmid Tables Additional Resources Mammalian organisms are exposed to millions of potential...Chemokines Chemokines, or chemotactic cytokines, are small proteins that can be secreted by immune cells and...X-associated protein - SHFM1 split hand/foot malformation (ectrodactyly) type 1 DSS1, ECD, SEM1, SHFD1...

...are sporadic, having no prior family history. A small percentage (5–10%) are familial ALS cases having...These familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic... Disease Huntington’s disease (HD) is a fatal autosomal dominant genetic disorder that causes the progressive...difficulty speaking and swallowing. HD is caused by abnormal expansion of a repeating CAG triplet series within...mutated HTT gene will have huntingtin proteins with abnormally long polyglutamine sequences. These glutamine-rich...medication and surgery. PD primarily involves the malfunction and death of dopamine-producing neurons in the... specific known cause) nature of the disease, a small portion of cases can be attributed to known genetic...

...RPS6KA2 RPS6KA3 RPS6KA6 Ribosomal protein S6 kinase RPS6KB RPS6KB1 RPS6KB2 Ribosomal protein S6 kinase B1...collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active Ras is the most...Plasmids Ras Gene List Resources Background Ras is a small GTPase and is a member of the G protein (guanine...addition and removal of a phosphate group. Under normal physiological conditions GTPases play prominent...factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP EIF4EBP1 EIF4EBP2...Ras-related C3 botulinum toxin substrate (rho family, small GTP binding protein Rac) RAF AFAF BRAF RAF1 Serine...

...Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as...SARS-CoV-2 Plasmids Other Coronavirus Plasmids Mammalian Targets General Plasmid Tools Pooled Libraries...encoding SARS-CoV-2 proteins, (spike protein, S; small envelope protein, E; nucleocapsid protein, N; and... SARS-CoV-2 plasmids for E. coli , yeast, and mammalian expression, and spike protein...al., 2020). Cathepsin L plasmids - a cysteine endosomal protease that triggers proteolysis of SARS-CoV... I., Holmes, E. C., & Garry, R. F. (2020). The proximal origin of SARS-CoV-2 . Nature medicine, 26 (4)...

...localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based on the acyl carrier protein. ...SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag...CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control Plasmid CLIP-tag...Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag...MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid ACP... Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic role of the ...