We narrowed to 520 results for: pes

...conditioned medium by filtration through a 0.45 µm PES filter or centrifugation at >500 x g for 5 minutes...concentrations, may be optimal for different cell types. Before starting this procedure, be sure that the...the lines for transgene expression and/or other phenotypes. For example, perform Western blotting to screen...

...doi.org/10.1371/journal.pcbi.1006508 Vicens Q, Bourne PE (2007) Ten Simple Rules for a Successful Collaboration...

...prime editing enzyme with a fully active Cas9 enzyme. PE-Cas9 comprises an active Cas9 fused to the reverse...mutations in a variety of target genes and cell types. Their Nature Biotechnology paper showcases an improved...

...KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient...modified to only infect user-defined starter cell types, as well as to only spread monosynaptically from...RABV is an enveloped virus. In general, viral envelopes dictate infection of target cells through their...

...by filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen...

...mScarlet plasmids here!   Cao W.X., Merritt D.M., Pe K., Cesar M., Hobert O. (2024) Comparative analysis...studies of E3 ligase cereblon By Mike Lacy Several types of drugs target the ubiquitin E3 ligase cereblon...

... license.   Find pYtags plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements...pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1...

...multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for nuclei...binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on constant...region. Isotypes The mammalian immune system produces five classes of antibodies, known as isotypes. An isotype...following table describes the five human isotypes. Some isotypes naturally form multi-antibody units, while...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling... applications, and is covered in the following Isotypes section. The variable region of the antibody is...“heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and...

...the eye and other tissues All Serotypes If an AAV is available in serotypes AAV1, AAV2, AAV5, AAV6, AAV8... that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...repository and request packaging in one of seven serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV11, or AAVrg... of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde Retrograde...AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed on the Function section...

... genetic material across a wide range of tissue types with minimal immune response in vivo. They have ...serotype. A helper plasmid containing adenoviral or herpesviral helper genes. These genes are not present in ...AAV vector production suitable for a variety of serotypes and applications. Read our AAV vector guide for...Service to produce AAV vectors for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2...AAV Rep and Cap genes for packaging in specific serotypes. Some also include adenoviral helper genes (E4...presence of a "helper" virus, such as adenovirus or herpes simplex virus, to replicate in the host cell and...

...viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1, AAV2, AAV5, AAV6, ...can be used to compare the tropism of different serotypes. In addition to the standard 100 µL sample size...be used to validate the performance of various serotypes before conducting larger scale experiments. For...#37825) Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made...#50465) Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made...

... that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...plasmids to be packaged in their choice of seven serotypes, resulting in over 28,000 possible choices! By...the Addgene repository in your choice of seven serotypes and four volumes. Details Scale and Titer Packaged...Serotype Packaged on Request can be ordered in seven serotypes: AAV1 AAV2 AAV5 AAV6 AAV8 AAV11 AAVrg Want more...before choosing a serotype? Learn more about AAV serotypes and tissue tropism on Addgene's blog . All-in-...

...many different cell types in the body during development. Unlike other cell types, each stem cell has ...stem cells, can differentiate into multiple cell types, but are typically limited to related cell lineages...somatic stem cells or fully differentiated cell types. Browse Addgene's collection of Differentiation...Lineage Reprogramming Reveals Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell...Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6. Buganim...

...expanded the CRISPR toolbox to include many different types of edits, including point mutations, base changes...kidney 293 (HEK293) cells. For more difficult cell types, you may need to look into other options. Browse...with, and are good choices for more difficult cell types like primary cells. For CRISPR delivery, lentiviral...cells. You can direct expression to specific cell types by using cell-specific promoters, which is useful...edit. CRISPR editing produces several possible genotypes within the resulting cell population. Some cells...

...zebrafish ( Danio rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater...Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain - Alex Schier Lab Other ...ablation of previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically...carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative...

...brain parenchyma or subretinal space Serotypes The following serotypes were developed in Arun Srivastava’...Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service...plasmid below is available in each of the above serotypes and buffers. Please click on the plasmid name ...

...number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16 subtypes based ... the targeting capability of Cas9 to make other types of modifications at specific loci. Activate or Repress...

...Burkholderia cenocepacia Miguel Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL (Quorum sensing molecule) Escherichia...to create, store, and replicate plasmids of all types, but beyond that, researchers also use bacteria ...Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence ...

...variation affects genome function to influence phenotypes....predict, characterize, and map how genome function shapes phenotype, and how these processes are affected...impact of genomic variants on genome function and phenotypes. Plasmids The collection will grow as the IGVF...

... use as a set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering ...here to view all pooled libraries. Pooled Library Types Barcoding CRISPR Screening Surface Display cDNA ...here to view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...