Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 521 results for: pes

Showing: 21 - 40 of 521 results

  1. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...conditioned medium by filtration through a 0.45 µm PES filter or centrifugation at >500 x g for 5 minutes...concentrations, may be optimal for different cell types. Before starting this procedure, be sure that the...the lines for transgene expression and/or other phenotypes. For example, perform Western blotting to screen...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...cloning of PCR products. Finney M, Nisson PE, Rashtchian A. Current Protocols in Molecular Biology...plasmids may be successfully adapted to numerous cell types using preferred culture conditions and transfection...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...prime editing enzyme with a fully active Cas9 enzyme. PE-Cas9 comprises an active Cas9 fused to the reverse...mutations in a variety of target genes and cell types. Their Nature Biotechnology paper showcases an improved...

  5. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient...modified to only infect user-defined starter cell types, as well as to only spread monosynaptically from...RABV is an enveloped virus. In general, viral envelopes dictate infection of target cells through their...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...by filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen...

  7. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...mScarlet plasmids here!   Cao W.X., Merritt D.M., Pe K., Cesar M., Hobert O. (2024) Comparative analysis...studies of E3 ligase cereblon By Mike Lacy Several types of drugs target the ubiquitin E3 ligase cereblon...

  8. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... license.   Find pYtags plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements...pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1...

  9. Antibody Guide

    Type
    Collection
    ...multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for nuclei...binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on constant...region. Isotypes The mammalian immune system produces five classes of antibodies, known as isotypes. An isotype...following table describes the five human isotypes. Some isotypes naturally form multi-antibody units, while...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling... applications, and is covered in the following Isotypes section. The variable region of the antibody is...“heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and...

  10. CRISPR Guide

    Type
    Guide
    ...RNA-binding protein to stabilize pegRNAs Twin PE - two PEs make complementary edits to opposite strands...stability PEmax - optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced... Overview of prime editing Popular prime editing (PE) tools include: PE2 - improved RT enzyme efficiency...employed to knock out target genes in various cell types and organisms, but modifications to various Cas ...are rare cases where Acr can work with multiple types, such as AcrVA3.1, which inhibits both Cas12a and...about 8 bp, specified by a gRNA. There are two main types of base edits: Base transitions - edit to same classification...then carries out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration...

  11. AAV Viral Preps

    Type
    Collection
    ...the eye and other tissues All Serotypes If an AAV is available in serotypes AAV1, AAV2, AAV5, AAV6, AAV8... that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...repository and request packaging in one of seven serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV11, or AAVrg... of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde Retrograde...AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed on the Function section...

  12. Serotype Testing AAV

    Type
    Collection
    ...viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1, AAV2, AAV5, AAV6, ...can be used to compare the tropism of different serotypes. In addition to the standard 100 µL sample size...be used to validate the performance of various serotypes before conducting larger scale experiments. For...#37825) Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made...#50465) Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made...

  13. AAV Packaged on Request

    Type
    Collection
    ... that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...plasmids to be packaged in their choice of seven serotypes, resulting in over 28,000 possible choices! By...the Addgene repository in your choice of seven serotypes and four volumes. Details Scale and Titer Packaged...Serotype Packaged on Request can be ordered in seven serotypes: AAV1 AAV2 AAV5 AAV6 AAV8 AAV11 AAVrg Want more...before choosing a serotype? Learn more about AAV serotypes and tissue tropism on Addgene's blog . All-in-...

  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...many different cell types in the body during development. Unlike other cell types, each stem cell has ...stem cells, can differentiate into multiple cell types, but are typically limited to related cell lineages...somatic stem cells or fully differentiated cell types. Browse Addgene's collection of Differentiation...Lineage Reprogramming Reveals Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell...Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6. Buganim...

  15. Plan Your Experiment

    Type
    Collection
    ...expanded the CRISPR toolbox to include many different types of edits, including point mutations, base changes...kidney 293 (HEK293) cells. For more difficult cell types, you may need to look into other options. Browse...with, and are good choices for more difficult cell types like primary cells. For CRISPR delivery, lentiviral...cells. You can direct expression to specific cell types by using cell-specific promoters, which is useful...edit. CRISPR editing produces several possible genotypes within the resulting cell population. Some cells...

  16. Using a Light Microscope Protocol

    Type
    Protocol
    ...dish. Microscopes come in a huge range of shapes and sizes - from phone-sized, foldable microscopes that...Introduction Microscopes are emblematic of biological research and are found in many different types of laboratories...accessible) to massive transmission electron microscopes that allow us to see cellular components with...protocol, we'll focus on one of the most common types that you're likely to encounter: the compound light...light microscope. As the name suggests, light microscopes take advantage of the physical properties of ...you may wish to record your observations. Some microscopes have digital cameras attached to them that allow...capture images directly to a computer, but many microscopes do not and you may have to dust off your drawing...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ...zebrafish ( Danio rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater...Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain - Alex Schier Lab Other ...ablation of previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically...carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative...

  18. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...brain parenchyma or subretinal space Serotypes The following serotypes were developed in Arun Srivastava’...Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service...plasmid below is available in each of the above serotypes and buffers. Please click on the plasmid name ...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16 subtypes based ... the targeting capability of Cas9 to make other types of modifications at specific loci. Activate or Repress...

  20. Bacterial Expression Systems

    Type
    Collection
    ...Burkholderia cenocepacia Miguel Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL (Quorum sensing molecule) Escherichia...to create, store, and replicate plasmids of all types, but beyond that, researchers also use bacteria ...Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence ...
Showing: 21 - 40 of 521 results