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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 254 results for: sms

Showing: 21 - 40 of 254 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... aeruginosa . Anti-CRISPR genes employ varied mechanisms to block CRISPR. Some prevent the CRISPR-Cas ...Wiedenheft B, Maxwell KL, Davidson AR. 2015. Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...structures available in the PDB. Parton DL, Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans... the yeast vacuole, as well as to examine the mechanisms behind autophagic pathways. Rosado, et al. Autophagy... both in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly...has revolutionized genome editing in multiple organisms thanks to its simple, easily modifiable RNA-guided...screens required to identify correctly modified organisms has limited routine use of this technology. To...conditionally knock down proteins in a variety of model organisms. As long as it was expressed in a cell that also... mutagenized proteasome subunit PSMB5 to find mechanisms of resistance to a proteasome inhibitor; CRISPR-X...

  3. Plan Your Experiment

    Type
    Collection
    ...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed to specifically...calcium sensors (fRCaMP1/2, GECO1/2) The kinetic mechanisms of fast-decay red-fluorescent genetically encoded...

  6. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR...

  7. CRISPR Plasmids and Resources

    Type
    Collection
    ...collections feature plasmids for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila...

  9. Adenovirus Plasmids

    Type
    Collection
    ...adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and yeast. Two plasmids...

  10. Zebrafish Plasmid Collection

    Type
    Collection
    ...screening fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected...

  11. TALEN Plasmids and Kits

    Type
    Collection
    ...Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector...

  12. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ... possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate gene expression) that...eight two-input RSMs and three three-input RSMs. The plasmids used to construct these RSMs are listed in... not. How might one implement RSMs? In our study (1), we implement RSMs in E. coli with two plasmids (...two-input GRSMs use the same recognition site array on the register, and all three-input GRSMs use the ...these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence configuration...by temporally ordered biochemical events. How do RSMs work? The state of an RSM is defined by the sequence...expressed in each state. To help researchers adapt GRSMs for their research, we provide MATLAB-based software...

  15. Rett Syndrome

    Type
    Collection
    ...differentiated cells that can be used to understand the mechanisms of the disease and for the development of successful...

  16. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...genetic code is used by most organisms on Earth, but different organisms have different preferences for...codons are used preferentially in which organisms - organisms have codon usage bias. You can find many... do about them? Read on to find out!   Why do organisms have different codon usage biases? The reasons...reasons for varied codon preferences among organisms aren’t completely understood, but some possible reasons... strains. Adaptation to changing conditions - Organisms often need to express genes at different levels...While codon preferences can be very useful for organisms, they can be problematic for researchers trying...can find a variety of databases showing various organisms’ codon preferences online). Even if the gene is...

  17. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... posts: Five Popular Model Organisms, Part 1 Five Popular Model Organisms, Part 2 ...providing access to genotypic study of diverse organisms. Methods for genetic manipulation still need to... transform, store, and share all of these new organisms continues to be challenging. Organism specific... created under this programs via Addgene. New organisms for genotype to phenotype studies New organismal...to tag and curate reagents from these emerging organisms so scientists can easily find the useful new reagents... Saccharomyces cerevisiae (both fine research organisms) -  perhaps you’ll choose to study Crepidula atrasolea...social bonds that they form including genetic mechanisms that control who a female wants to mate with ...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...responsible for bioluminescence found in a variety of organisms, ranging from bacteria to aquatic animals to insects...

  19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double stranded...can consider the microbiome as a collection of organisms, their genes, and metabolic processes, occupying... diseases have connections to either specific organisms or organism/gene interactions with the host. This... al., 2014). These could be genes, specific polymorphisms, including antibiotic resistance genes. This...This approach successfully targeted specific organisms when using bacteriophages or mobile plasmids in a...Pseudomonas species Expanding this tool to other organisms, the Ji lab brought genome and base editing to...genome engineering remains challenging for most organisms in the genus. Work from the Sun lab demonstrates...

  20. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... a variety of functions that allow their host organisms to thrive in competitive environments. The genes...encode genes that allow the host to infect other organisms. If you’ve ever wondered why E. coli is safe to...means to deliver DNA and other molecules to host organisms. For instance, vectors derived from the Agrobacterium...like by living where no one else wants to. Some organisms manage this with plasmids that allow them to eat... eat unusual substrates that other organisms can’t use. Other plasmids offer resistance to toxic substances... which protect the host from attacks by other organisms. To conserve energy, these specialized degradative...
Showing: 21 - 40 of 254 results