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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 443 results for: DUR

Showing: 381 - 400 of 443 results

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...K., & Carroll, D. (2013). Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes...

  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...simultaneously measuring the activities of CaMKII and RhoA during induction of structural plasticity in single dendritic...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...neuronal loop which is activated and strengthened during memory formation and memory retrieval. In neuronal...

  9. Molecular Biology Reference

    Type
    Guide
    ...bacteria, which can uptake plasmids and amplify them during cell division. Because bacteria are easy to grow...Specifically, A pairs with T and C pairs with G. During replication, DNA unwinds and the DNA polymerase...utilizes modified fluorescently tagged nucleotides. During illumina NGS, a long piece of DNA is first fragmented...Addgene all incoming plasmids are sequenced with NGS during our quality control process . NGS allows us to ...

  10. CRISPR Guide

    Type
    Guide
    ...modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically or genetically ...cytidine to uridine, which is read as thymidine during subsequent DNA repair, creating a C to T change...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

  11. Cloning

    Type
    Guide
    ...are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ligase. Restriction...easy to add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...

  12. Optogenetics Guide

    Type
    Guide
    ...functions. Example schematic of an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed...outside of the brain, rendering the experimental procedure less invasive. Different activation wavelengths...

  13. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...vector is determined by the packaging plasmid used during production, as it carries the Cap region responsible...adeno-associated virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector production...

  14. Chemogenetics Guide

    Type
    Guide
    ...Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM,...

  15. Adenovirus Guide

    Type
    Guide
    ...HER911 cells throughout viral vector production. During experiments, GFP can be used to sort cells infected...

  16. Lentiviral Vector Guide

    Type
    Guide
    .../10.1128/jvi.72.11.8463-8471.1998 PMID: 9765382 Durand, S., & Cimarelli, A. (2011). The inside out of ...

  17. Antibody Guide

    Type
    Guide
    ...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...

  18. CRISPR Library Amplification

    Type
    Protocol
    ... for all steps. Procedure Day 1 Add 200 ng DNA to each 50 µL aliquot of thawed Endura Duos on ice. Flick...representation of individual plasmids in the pooled library during amplification. This protocol is designed to be ... µL electrocompetent cells (Default: 4 tubes of Endura Duos, Lucigen, 60242-1) Alternatives include Stbl4... cm cuvette, 1.8 kV, 1 pulse. Aliquot 25 µL DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation...contain a total of 5 mL (3 mL SOC + 2 mL transformed Endura from two separate transformations). Pro-Tip Do ...mix when removing SOC containing transformed DNA-Endura from cuvette. Shake four 14 mL Vented Falcon Tubes...pulling motion Take care not to split or gouge agar during the scraping process. Add each scrape into a 50...

  19. Lentivirus Production

    Type
    Protocol
    ...polyethylenimine (PEI) transfection protocol. This procedure can be modified for alternative packaging cell...that are below passage 15 for viral production. Procedure Seed 293T packaging cells at 3.8×10 6 cells per...DNA tube. Incubate the mixture 12–15 min at RT. During the incubation, add 10 mL of DMEM Complete to a... pellet any packaging cells that were collected during harvesting. Filter supernatant through a 0.45 μm...

  20. AAV Production in HEK293 Cells

    Type
    Protocol
    ...glucose, lower FBS media causes less filter clogging during harvesting. The addition of sorbitol has been shown.... Autoclave or sterile filter. Pro-Tip Stirring during the cooling period is recommended or the solution...overgrow your cells. Pass the cells twice a week during the maintenance phase and do not allow cells to.... Thaw a new vial of cells after 30 passages. Procedure Trypsinize and resuspend the HEK293T cells from...
Showing: 381 - 400 of 443 results