We narrowed to 445 results for: DUR

...evidence,” and to speak out in unprecedented force during the 2015 federal election.   Even as we marched...

..., it is much easier to genetically modify cells during the reprogramming process as they are propagated...

... Note: depending on which method you use, the procedure will vary slightly.   Two Golden Gate options...

... plasmid information. Enter plasmid information During the remainder of the process, you will enter plasmid...

...glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil editing, and instead introduced...

...K., & Carroll, D. (2013). Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes...

...resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second copy of the...

...neuronal loop which is activated and strengthened during memory formation and memory retrieval. In neuronal...

...simultaneously measuring the activities of CaMKII and RhoA during induction of structural plasticity in single dendritic...

...bacteria, which take up plasmids and amplify them during cell division. Because bacteria are easy to grow...advantage of the process of DNA replication in vitro. During replication, the DNA helix is unwound by a helicase...incorporation of modified, fluorescently-tagged bases during in vitro DNA replication in addition to the normal...utilizes modified, fluorescently-tagged nucleotides. During Illumina NGS, a long piece of DNA is fragmented...Addgene, all incoming plasmids are sequenced with NGS during our quality control process . NGS allows us to ...

...or which are both blunt-ended, can then be fused during a ligation reaction with T4 DNA ligase. Restriction...easy to add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...cloning is a recombination-based cloning method. During Gateway, moving a piece of DNA from one plasmid...

...modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically or genetically ...cytidine to uridine, which is read as thymidine during subsequent DNA repair, creating a C to T change...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...Optogenetics Plasmid Collection . An optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed...outside of the brain, rendering the experimental procedure less invasive. Different activation wavelengths...

...vector is determined by the packaging plasmid used during production, as it carries the Cap region responsible...adeno-associated virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector production...

...polymerase. Figure 1: Simplified promoter region during transcription. Created with BioRender.com. DNA ...

...Snowball, A., Knauss, S., von Schimmelmann, M., During, M. J., Lignani, G., Schorge, S., Young, D., Kullmann...

...HER911 cells throughout viral vector production. During experiments, GFP can be used to sort cells infected...

.../10.1128/jvi.72.11.8463-8471.1998 PMID: 9765382 Durand, S., & Cimarelli, A. (2011). The inside out of ...

...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...

... for all steps. Procedure Day 1 Add 200 ng DNA to each 50 µL aliquot of thawed Endura Duos on ice. Flick...representation of individual plasmids in the pooled library during amplification. This protocol is designed to be ... µL electrocompetent cells (Default: 4 tubes of Endura Duos, Lucigen, 60242-1) Alternatives include Stbl4... cm cuvette, 1.8 kV, 1 pulse. Aliquot 25 µL DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation...contain a total of 5 mL (3 mL SOC + 2 mL transformed Endura from two separate transformations). Pro-Tip Do ...mix when removing SOC containing transformed DNA-Endura from cuvette. Shake four 14 mL Vented Falcon Tubes...pulling motion Take care not to split or gouge agar during the scraping process. Add each scrape into a 50...