...plasmid encoding your insert of interest Reagent Preparation DMEM Complete (also known as D10): 10% v/v FBS...Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Prepare a mixture of the three transfection plasmids into...empirically determined. Once a batch of PEI is prepared, transfect cells with a fluorescent plasmid using...

...their resistance genes, as one does not need to prepare separate batches of antibiotic-containing agar....or other antibiotic resistance) Procedure Day 1 Prepare carbenicillin to a concentration of 1 mg/mL – 4...

...many different experimental procedures, such as minipreps and RNA extractions. Plus, centrifuges are useful...

...sell competent cells, which come frozen and are prepared for optimal transformation efficiencies upon thawing...

...image of that object! Equipment Light Microscope Prepared Slides (or other sample that can fit on a microscope...

...and concentration of PCR product. Master Mix Preparation Multiply the volume of each reagent by the number...

...to room temperature (RT) before use. Reagent Preparation 1 M sodium phosphate monobasic monohydrate (NaH...

...Protocol Before starting this protocol, make sure to prepare solution D (see reagent section above for the recipe...