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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 466 results for: Sele

Showing: 381 - 400 of 466 results

  1. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker or cloning...

  2. Recombinases AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...

  3. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker or cloning...

  4. Antibody Plasmid Collection

    Type
    Collection
    ... human antibodies Synthetic Human Antibodies: Selecting and Evolving Functional Proteins. METHODS: A Companion...functional analysis of scFv antibody fragments selected from Tomlinson I+J phagemid libraries. Immunol...

  5. Worm Expression Resources

    Type
    Collection
    ...templates that incorporate a self-excising drug selection cassette (SEC). More information regarding this...vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John...

  6. Plant Plasmids and Resources

    Type
    Collection
    ...technology or the Plant CRISPR Resources for a full selection of plasmids expressing different functional variants...Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation...

  7. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker or cloning...

  8. Control AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ...al. (PMID: 21179091), as well as (v) distinct selection cassettes on pTAL7a and pTAL7b for enrichment ... as an alternative. Based on transient double-selection of transfected cells, the system has successfully...

  11. Optogenetics AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...

  12. The Pleiades Promoter Project

    Type
    Collection
    ... regulatory toolbox of MiniPromoters to drive selective expression in the brain.... regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U ...

  13. Bacterial Expression Systems

    Type
    Collection
    ...42°C). Transposition is efficient enough that selection is not required. pET-HIS-Sangamo 40786 TALEN Gang...includes the SacB gene, allowing for negative selection on 5% sucrose. Other empty vectors for protein...

  14. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas9 that can cut, activate, interfere, or nick. Selection , such as Puromycin or EGFP Cloning enzyme used... Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875 Bacteria BsaI none S. pyogenes...

  15. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...first gel lane (as in Figure 2). Select Analyze . Select Gels . Select Select First Lane . Drag the box to...to the next lane. Select Analyze . Select Gels . Select Select Next Lane . Repeat for all lanes. Plot ...protein bands as follows: Figure 3 Select Analyze . Select Gels . Select Plot Lanes . One graph per lane ...the Chart Editor , select the Customize tab. Select the Series drop down menu. Select the Trendline checkbox... lane. Import the gel image into ImageJ. Select File . Select Open . Choose the location of the file to... open. Change the image type to 8-bit . Select Image . Select Type . Choose 8-bit . Determine each lane...the bottom of each peak. Select the wand tool. Fill in each peak by selecting the center of the peak. ...

  16. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...for effective over-agar selection has been empirically determined. See selection curve below. Carbenicillin..., plated over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using...1 mg/mL and 2 mg/mL plates and effective selection. Selection Curve of Transformed E. coli after Over-...methodology for plating antibiotic over-agar for the selection of transformed E. coli . Over-agar spreading of...easy for an investigator to conveniently plate and select transformed cells containing plasmids differing...agar. This protocol will focus specifically on selection with carbenicillin; however it can be adapted ...adapted for any antibiotic through the use of a selection curve to determine optimal antibiotic concentration...

  17. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... target selection are continuously being improved. Below are suggestions for target selection. Use an ...concentration F.3 Protocol for lentiviral infection and selection G. Safety H. References H.1 Published articles...encoded in pLKO.1 allows for convenient stable selection. Figure 1 : Map of pLKO.1 containing an shRNA ...puromycin. Puro R Puromycin resistance gene for selection of pLKO.1 plasmid in mammalian cells. sin 3’LTR...replication. Amp R Ampicillin resistance gene for selection of pLKO.1 plasmid in bacterial cells pUC ori pUC...Determining the Optimal 21-mer Targets in your Gene Selection of suitable 21-mer targets in your gene is the...an siRNA selection tool to determine a set of top-scoring targets for your gene. For example, the Whitehead...

  18. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...enables selection of a stable cell culture after transduction. Performing antibiotic selection on transduced...This is the beginning of the selection process, which will begin the selection of a stable cell pool. Observe...transducibility of the cell line used, this antibiotic selection may be a vital step for obtaining a population...Remove media, replace with fresh media containing selection reagent Day 3–14: Change media as needed Day 14...Lentivirus preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation...Before beginning, determine the optimal dose of selective reagent for your target cell line. To do this,...resulting in massive cell death upon antibiotic selection. The number of cells transduced should be enough...

  19. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... thought on using selective medium throughout subsequent experiments. When selective medium is used, it...was used for the initial selection). Addgene recommends that the use of selective medium throughout experiments...site(s) of proviral integration across cells. Selective pressure on this heterogeneous cell pool could...the polyclonal cell pool is always undergoing selection, it’s best to generate monoclonal cell lines from... sure that the cell pool has been sufficiently selected with the appropriate antibiotic so that every ...biological effects on the cell line. In the absence of selective medium, there is a chance that the integrated ... Some researchers choose to maintain cells in selective medium at a reduced antibiotic concentration (...
Showing: 381 - 400 of 466 results