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We narrowed to 625 results for: Emb

Showing: 401 - 420 of 625 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used to modify genomic loci that...SpCas9 (Amrani et al., 2018). When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic...

  2. Hot Plasmids - August 2020

    Type
    Blog Post
    ...with plasmids encoding FPs tagged with the plasma membrane targetin sequence derived either from p63(1-29...non-specific labeling. For labeling the plasma membrane, they tested different target sequences for improved...

  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...Members of the bacterial world produce an assortment toxins to claim territory or kill competing microorganisms... (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a short-lived RNA...

  5. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...for Excellence in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of...for Excellence in Molecular Plant Sciences in September 2019 as a Ph.D. candidate. Her research focuses...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...harboring this mutation are deficient in outer membrane protease VII, which reduces proteolysis of the...pLemo (CamR) Expression of toxic, insoluble, or membrane proteins  T7 Express   IPTG-inducible expression...

  9. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...empathy, a willingness to listen, and an ability to remember the right stories at the right time. Like many...morefamousquotes.com A good listener absorbs and remembers and makes the teller feel heard. I am not always...

  10. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...recommendations.  As you optimize your protocol, always remember to include controls to show that your antibodies...sample).  When choosing a primary antibody, first, remember that antibodies recognize very specific 3-dimensional...

  11. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several... guest blogger! Daria Shcherbakova is a faculty member at Albert Einstein College of Medicine. Her expertise...

  12. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...cells.   GFP is big GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter...Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells." Science 296.5569 ...

  13. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... Symposium 2015. I personally participated as a member of the Career Paths Panel on the second day of ...people from all backgrounds are truly included as members of their research communities and are treated equitably...

  15. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute.... While this seems obvious, it is important to remember that the same is true when designing gRNAs for...

  16. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian expression systems...vivo cis-regulatory interactions in the chicken embryo. BioRxiv: https://doi.org/10.1101/135525. Find...

  17. A Control for All Seasons

    Type
    Blog Post
    ...and equal protein transfer from the gel to the membrane. A loading control is critical for data interpretation.... Always probe the loading control on the same membrane that the target protein was measured on.    Flow...

  18. Antibodies 101: Validation

    Type
    Blog Post
    ...Show suitability for an intended application - Remember, just because your antibody performs as expected...is just as helpful as knowing when it will. And remember, antibodies are like puppies. Just because an ...
Showing: 401 - 420 of 625 results