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Showing: 631 - 660 of 707 results

  1. TALEN Guide

    Type
    Collection
    ...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...

  2. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging...for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid,...repository for AAV vector production. ID Plasmid Description PI Return to top Other Resources Addgene's Viral...

  3. Lentivirus Plasmids

    Type
    Collection
    ... for gRNA expression . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty...Lentiviral Packaging Plasmids ID Plasmid Generation Description PI 1732 pCPRDEnv 2nd Part of the FELIX vector...Popular Lentiviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

  4. Neurodegeneration Research Collection

    Type
    Collection
    ...2023) See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders...

  5. Genetic Code Expansion

    Type
    Collection
    ...UGA). The amber codon is the rarest codon in Escherichia coli , which makes it the most commonly used ...Liu 164081 pCMV-MfPylRS (human-opti) PylRS M. flavescens acetyl-lysine Mammalian Tao Liu 164195 pCMV-MtAcKRS...Liu 164196 pCMV-MfBulKRS (human-opti) BulKRS M. flavescens acetyl-lysine Mammalian Tao Liu 164579 pEVOL-...non-canonical amino acid incorporation. ID Strain Description PI 48998 C321.ΔA All TAG sites changed to TAA...

  6. Optogenetics AAV Preps

    Type
    Collection
    ...Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent protein ...

  7. Retrograde AAV viral preps

    Type
    Collection
    ... pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor... pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine... pAAV-hSyn-GRAB_NE1h Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3...and dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent...

  9. Plan Your Experiment

    Type
    Collection
    ...validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment.... mRNA and Proteins In addition to the methods described above, you can also directly deliver mature mRNA...endosomal approach. Similar to the mRNA method described above, this method is also transient, as the RNPs...

  10. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to... plasmids from this depositor's lab. Plasmid Description Vector Type Selectable Marker PI Publication ...

  11. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...10.1038/nprot.2009.98. Epub 2009 Sep 17. PubMed . Description Use of the OPEN method requires OPEN pools (available...using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove et al., Nature...

  12. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...neutralizing activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral ...

  13. Synthetic Biology - Overview

    Type
    Collection
    ...Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering of biological systems...Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate...

  14. Antibody Plasmid Collection

    Type
    Collection
    ...create and express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy...for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions...

  15. Viral Production

    Type
    Collection
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre....

  16. CRISPR Plasmids - Tagging

    Type
    Collection
    ...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information... the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell...

  17. CRISPR References and Information

    Type
    Collection
    ...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene...

  18. Rett Syndrome

    Type
    Collection
    ...lab determines a minimally functional MECP2 that rescues neurological defects of Rett syndrome. (Link opens...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ... nuclear receptor subfamily 2, group E, member 3 ESCS, MGC49976, PNR, RNR, RP37, rd7 NR2F1 nuclear receptor...Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories...immune response. Over 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can...immune response. Over 60 interleukins have been described. Symbol Name Synonyms EBI3 Epstein-Barr Virus ...and standardized building blocks: 14 different fluorescent proteins, 10 mammalian promoters and enhancers...Plasmids 101: Monitoring Cell Mobility Using Fluorescent Proteins The Challenges of Cell Culture External...

  20. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations... enhancers for specificity and brightness of fluorescent labeling across brain regions and populations...

  22. Synthetic Biology - Algal

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  23. Synthetic Biology - Bacterial

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  24. Synthetic Biology - Fungal

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  25. Synthetic Biology - Mammalian

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  26. Synthetic Biology - Plant

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  27. Synthetic Biology - Worm

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  28. Synthetic Biology - Yeast

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  29. Tags and Other Markers

    Type
    Collection
    ...our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity Recommended...

  30. Synthetic Biology - Strains

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication...
Showing: 631 - 660 of 707 results