1,167 results

1. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

2. Lentivirus Production

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   Protocol

3. Colony Formation Titering Assay

   Type

   Protocol

4. Fluorescence Titering Assay

   Type

   Protocol

5. General Transfection

   Type

   Protocol

6. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

7. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

8. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

9. AAV Production in HEK293 Cells

   Type

   Protocol

10. Protocol - How to Perform a Diagnostic Digest

    Type

    Protocol

11. Protocol - How to Run an Agarose Gel

    Type

    Protocol

12. Protocol - Bacterial Transformation

    Type

    Protocol

13. Protocol - How to Create a Bacterial Glycerol Stock

    Type

    Protocol

14. Protocol - How to Ligate Plasmid DNA

    Type

    Protocol

15. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type

    Protocol

16. DNA Quantification

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    Protocol

17. Protocol - How to Inoculate a Bacterial Culture

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    Protocol

18. Protocol - How to Purify DNA from an Agarose Gel

    Type

    Protocol

19. Protocol - How to Streak a Plate

    Type

    Protocol

20. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

21. Gibson Assembly Protocol

    Type

    Protocol

22. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type

    Protocol

23. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type

    Protocol

24. Ligation Independent Cloning

    Type

    Protocol

25. What is Polymerase Chain Reaction (PCR)

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    Protocol

26. Protocol - How to Design Primers

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    Protocol

27. Protocol - pLKO.1 – TRC Cloning Vector

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    Protocol

28. Protocol - How to Perform Sequence Analysis

    Type

    Protocol

29. Protocol - Over-Agar Antibiotic Plating

    Type

    Protocol

30. Video Library

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    Protocol

31. CRISPR Library Amplification

    Type

    Protocol

32. AAV ddPCR Titration

    Type

    Protocol

33. Lentivirus ddPCR Titration

    Type

    Protocol

34. Kit Free RNA Extraction

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    Protocol

35. Weighing Reagents Protocol

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    Protocol

36. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type

    Protocol

37. Lab Safety for Biosafety Levels One and Two

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    Protocol

38. Pipetting Protocol

    Type

    Protocol

39. Water Bath Protocol

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    Protocol

40. Using a Light Microscope Protocol

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    Protocol