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We narrowed to 438 results for: ERG

Showing: 101 - 150 of 438 results

  1. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...sensitivity and detection - since they frequently undergo conformational changes after target binding, aptamers... biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and... L. “Aptamer based electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2...

  2. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... was designed in response to requirements that emerged from intensive engagement with a wide range of ...research computing. The two guiding principles emerging from the engagement with the Wisconsin research...fundamental driver of these principles and has emerged as a byproduct of connectivity. Between 2012 and...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...fashion while allowing for creative solutions to emerge. They enjoy the fact that each new challenge will...amount of time can sometimes become cauldrons on the verge of boiling. On other occasions, clients can be excessively...thoughtful planning and engagement, leaving mental energy to enjoy the more rewarding and educational parts...

  4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...approach, Zhang et al. turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from...Young WB, Khalili K, Hu W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent...Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger et al., 2017).     Figure 3: Comparison...C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate Route for Adeno-.... https://doi.org/10.1007/s11064-004-6881-1 Taschenberger, G., Tereshchenko, J., & Kügler, S. (2017). ...

  6. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...there is a lack of expert mentorship during undergraduate and graduate studies. Students also need more... a rapidly changing, globalized environment re-emerging from international financial struggle, our new...mentorship in the field of Biomedicine to 78 undergraduate/graduate students from 5 independent universities...

  7. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...both methods did a more comprehensive gene list emerge that accurately reflected the scope of genes known... straightforward positive selection of plants undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly...microscopic plants must be visually identified as undergoing RNAi through the fluorescent reporter and manually...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    ...has been demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move toward...Examining serotypes 1, 8 and 9, not only do they undergo retrograde and anterograde transport, but they ...avoid this rate limiting step and can immediately undergo replication and transcription. However, with scAAV...

  9. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...delivery methods, but since TEs insert mostly in intergenic regions, gene disruption is less of a concern...to a single location in bacterial genomes, the Sternberg lab developed the INTEGRATE (Insertion of Transposable...transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021) Contemporary Transposon Tools...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...substrate, luciferin, which releases energy as a photon. The photon energy is transferred to the acceptor fluorophore...pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab...fusion protein (referred to as dCas9-VPR) had a synergistic effect on target gene activation. The novel dCas9...rely on the principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla...

  12. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x...resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence Activated Cell Sorting...

  13. Tags and Other Markers

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control...

  14. Neuroscience

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control...

  15. TREAT-AD Plasmid Collection

    Type
    Collection
    ...disease (AD) by generating validated resources for emerging targets in AD and creating high-quality, well-...

  16. Antibodies

    Type
    Collection
    ... are expressed from plasmids. These antibodies undergo application-specific validation and consistent ...

  17. KLF Research Plasmids

    Type
    Collection
    ...Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About ...

  19. All Antibodies

    Type
    Collection
    ...recombinant antibodies. These monoclonal antibodies undergo application-specific validation and quality control...

  20. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion...

  21. E11 Bio PRISM Collection

    Type
    Collection
    ...non-profit focused research organization supported by Convergent Research (Link opens in a new window) , in collaboration...

  22. Synthetic Biology - Overview

    Type
    Collection
    ...of community-created BioBricks JBEI - Joint Bio-Energy Institute Synberc - Synthetic Biology Engineering...

  24. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...accounts for many, many lattes and espressos. The energy boost is well needed to keep up with all the awesome...great opportunity – so if you’re running out of energy, then it’s time for a coffee run! ...

  25. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...few examples are provided here for reference: Untergasser Lab Preparation of chemically competent cells... subsequent transformation protocols below: Untergasser Lab Transformation of chemically competent cells...

  28. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...effort was led by two amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School...Bourne charged the audience to use their useful energy and to choose institutions with allies engaged ...

  29. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study...and worked around the world, carrying out her undergraduate work at the University of London and the University...

  30. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...understand about Dopamine? DA is produced by specific dopaminergic neurons in a brain region called the substantia... Report endogenous DA release from a single dopaminergic fiber in mouse brain slices Demonstrate DA detection...

  31. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive when ...):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger, J., Song, WC. Complement and its role in innate...

  32. Antibody Plasmid Collection

    Type
    Collection
    ...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...

  33. Distribution to Industry

    Type
    Collection
    ...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...

  34. AAV Molecular Tools

    Type
    Collection
    ...transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter...

  37. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... Subsequent work found that both NaCl and non-detergent sulfobetaine 201 (NDSB-201) were required for ... PubMed. Is VP22 nuclear homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001 Aug;19(8)...

  38. Microbiology Resources

    Type
    Collection
    ...Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions...

  39. Worm Expression Resources

    Type
    Collection
    ...promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4-...

  40. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...human U2OS cells. Based upon this, they chose an intergenic DNA region (IDR3) as a reference loci to measure...measure inter-locus distances of loci pairs from intergenic regions, intronic regions, and a pericentromeric...

  42. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K. phaffii is....2017.941 Bernauer L., et al., Komagataella phaffi as Emerging Model Organisms in Fundamental Research. Front...

  43. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...LabCentral is a lab and office space shared by numerous emerging biotech companies. The space was modern with clean...When I heard about how LabCentral aims to enable emerging biotech companies, I was excited to go there because...

  44. How to Lead a Great Meeting

    Type
    Blog Post
    ...useful information, and invigoration - a renewed energy for continuing the project after leaving the meeting...you will be running collaborative, informative, energizing meetings all your own. Additional Resources...

  45. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...had some supervisory experience working with undergraduate students? On top of that, perhaps you were involved...career services offices. You might think only undergraduates use these services, but some of the general...

  46. TALEN Guide

    Type
    Collection
    ...Jens Boch at the Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published...

  48. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ... either. Coming from a strong background of undergraduate research straight to a lab career at a biotech...more! This role is a good fit for you if you are energized from interacting with others, and have strong ...

  49. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...control plasmid for expression in forebrain GABA-ergic interneurons (83895). See our Serotype Testing AAV...which targets expression of ChR2 to forebrain GAB-ergic interneurons through the mDlx enhancer element. ...

  50. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... luciferase substrate, the luciferase transfers energy to the FP, resulting in a fluorescent signal. Since...based on the principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase...
Showing: 101 - 150 of 438 results