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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 232 results for: SPL;

Showing: 101 - 150 of 232 results

  2. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...emission maximum. Note, you can also use the tool to display excitation spectra, which I excluded here for better...created through the actual APC-Cy7 fluorophore but displays an emission spectrum that overlaps with APC-Cy7...

  3. Institute for Protein Innovation

    Type
    Collection
    ...platform (Link opens in a new window) based on yeast display technology (Link opens in a new window) and an ...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Rajat Rohatgi 107855 pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G) TARDBP CMV ALS Rajat...Rajat Rohatgi 107860 pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV) TARDBP CMV ALS Rajat... VWM disease Petr Svoboda 121165 tau exon 9-12 splicing minigene MAPT CMV Parkinson's, FTD Stefan Stamm...ALS Agnel Sfeir 169375 pET3d-SF-DCTN1-TS DCTN1 SplitFIAsH, Strep, His T7 ALS, motor neuron disease Bernd...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... reverse transcription. The edited DNA strand displaces the unedited 5’ flap and the resulting heteroduplex...

  6. Worm Expression Resources

    Type
    Collection
    ...ubiquitous or a tissue-specific promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...membrane and gel during the transfer, while dark splotches could indicate dirty equipment, degraded or improperly...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...improved peroxidase reporter, APEX2, through yeast display. APEX2 has been shown to exhibit superior performance...functional complementation between two non-fluorescent split GFP fragments. When the two fragments, expressed...

  9. COVID-19 Resources

    Type
    Collection
    ...page. SARS-CoV-2 Pooled Libraries - Yeast surface display libraries of Spike (S) Ectodomain and RBD mutants...

  10. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGTGTCTTCAGGTCGTTGtcaacgctggaggaatTGTGTTGCGTTCTGGTAA crispld2 TAL3232 & TAL3233 TATCAGGAGGAGCTAGAAcccaacagcactaaacCCGACGCTCCTGTCCGAA...

  11. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...station if unwanted or biohazardous materials are splashed onto your face and/or get into your eyes. Wash...shaking or mixing, which may produce aerosols or splashes. These procedures should be done underneath a ...

  12. Caltech Systemic Capsids

    Type
    Collection
    ...strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive...

  13. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Knockout Human Olzmann 3rd ~10 13,920 Human lncRNA Splicing-targeting CRISPR Library 119977 Knockout Human...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ... of PEG solution to each 100 mL of supernatant. Split into 2 x 500 mL sterile bottles as needed. Add stir...cells were imaged with a 10X objective and were split 2 days before these images were taken. Last reviewed...

  15. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...More #agarart from our lab @SILS_UvA 🧬🧫 Soon on display @Micropia! #SciArt #MerryChristmas #happyholidays2018...

  19. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...important advance was quickly followed by even splashier news: Jiahao Sha of Nanjing Medical University...

  22. Editor's Choice, September 2016

    Type
    Blog Post
    ...Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva (a.k.a. FOP), that, ...

  28. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...was relieved to find out that the two hours were split up between several Addgene employees, who all made...

  34. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...creating v4 SunTag. Since the v4 system does not display protein aggregation, it’s recommended for most ...

  40. Validated gRNA Sequences

    Type
    Collection
    ...GLuc synthetic GATCTAGATACGACTCACTAT 68422 CRISPR-display S. pyogenes 26030444 Rinn gp78 H. sapiens GCCCAGCCTCCGCACCTACA...

  46. What the HEK?

    Type
    Blog Post
    ... fibroblast. However, Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent...
Showing: 101 - 150 of 232 results