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We narrowed to 492 results for: ARA-2

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Showing: 41 - 60 of 492 results

  1. CRISPR References and Information

    Type
    Collection
    ...Link opens in a new window) An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis, and rice. Proof-of-concept performed in zebrafish...

  2. Distribution to Industry

    Type
    Collection
    ...and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from ...Optimization Kit Using SUMO Protease Elution Protein Characterization Christopher Bahl A validation set of 96 unique...single-domain proteins that are structurally characterized (PDB-deposited), disulfide-free, mesophilic,...industry scientists. Please note this does not guarantee availability but lets us know what plasmids would...

  3. Deisseroth INTRSECT Collection

    Type
    Collection
    ...dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...delivering additional recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences... Mandelbaum G, Taranda J, Haynes TM, Hochbaum DR, Huang KW, Hyun M, Umadevi Venkataraju K, Straub C, Wang...payloads in target populations defined by multiple parameters. Depositor...payloads in target populations defined by multiple parameters. Figure 1: Examples of intersectional cell population...combination of genetic and/or anatomical-defined parameters, by placing two orthogonal recombinase (Cre and...Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron 102(3):636-652. PubMed (Link...

  4. AAV for Neuronal Tracing

    Type
    Collection
    ... and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein.... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  5. Institute for Protein Innovation

    Type
    Collection
    ... proteins are currently under development. Figure 2: Key adhesion proteins and receptor–ligand pairs bridge...IPI is providing access to high-quality, well-characterized antibodies in an open science model. Learn more...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...8661 p4455 FLAG-hPLIC-2 UBQLN2 Flag CMV ALS Peter Howley 8662 p4456 FLAG-hPLIC-2 NTF UBQLN2 Flag CMV ALS...196208 GB1-A11(2-196)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196209 GB1-A11(2-196, G38R)-Strep...196210 GB1-A11(2-196, D40G)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196211 GB1-A11(2-196, G175R...196212 GB1-A11(2-196, G189E)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196213 GB1-A11(2-52)-Strep ...TLS 1: hTLS.pBSKS(+) FUS T7 ALS David Ron 21828 TLS 2: hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C...ApoE Alzheimer's Sohail Tavazoie 51436 pGL3Basic-ME.2/ApoEpromoter APOE ApoE Alzheimer's Sohail Tavazoie...T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His T7 ALS Nicolas Fawzi 127196 pJ411 FUS 1-163...

  7. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient...15264811 Stachelhaus T, Mootz HD, Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal...

  8. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons... the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector Hillel...for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable...for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...hSyn promoter rtTA3 Wei Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter...expression and improved dynamic range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link...and the tet-controlled gene of interest from two separate vectors. Stable cell lines or transgenic animals...tissue-specific promoter and administer the transgene separately. All-in-one system: express both the transactivator...(Link opens in a new window) External Resources Takara: Tet-inducible Systems Overview (Link opens in ...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion...expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase expression plasmids. ID...

  11. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...AAV2 vectors in the mouse retina. Mol Ther . Feb;19(2):293-301. PMID: 21045809 Other citations include: ...sequencing of the final viral vector preparation. These viral vector preparations were produced with the pTR-UF11...Service , Addgene is producing ready-to-use viral preparations from the University of Florida for enhanced ...-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers...injection. It was identified by John Chiorini and characterized by Shannon Boye’s lab. AAV44.9(E531D) The AAV44.9... of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 Apr;27(3-4):127-142. PMID...Shannon Boye and cite Boye, et al. 2016. Impact of Heparan Sulfate Binding on Transduction of Retina by Recombinant...

  12. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...expressing Rep2 and Cap1 James M. Wilson 232199 pAAV2/2 AAV2 AAV packaging plasmid, expressing Rep2 and Cap2... (E4, E2a, and VA), eliminating the need for a separate helper plasmid during AAV production. You can ...

  13. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisomes Peroximal targeting signal (SKL) mCherry...Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles...GolgiCFP Golgi apparatus eNOS(1-33) CFP Alexandra Newton 36205 pmTurquoise2-Golgi Golgi apparatus B4GALT1(1...pmScarlet_Giantin_C1 Golgi apparatus Giantin mScarlet Dorus Gadella 129630 mTurquoise2-Giantin Golgi apparatus Giantin mTurquoise2...Golgi apparatus Giantin td5StayGold Atsushi Miyawaki 231777 pCMV-mGold2t-MannII-N-10 Golgi apparatus MannII... Golgi apparatus MannII mGold2s Francois St-Pierre 189773 pmScarlet3-Giantin_C1 Golgi apparatus Giantin...subcellular structures. To compare with a well-characterized marker's localization pattern, you should fuse...

  14. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...by Xiuyuan Ou, et al. that characterized the spike (S) protein of SARS-CoV-2 (Ou et al., 2020). The authors...ACE2 (Subbarao et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV-2, this...-Y (2020) Comparative tropism, replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-...Q, Wang J, Qian Z (2020) Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune...fast access to the tools required to study SARS-CoV-2, but with many academic research labs closed during...biobanks, are crucial for the advancement of SARS-CoV-2 research because they provide rapid access to validated...Many of the reagents used to investigate SARS-CoV-2 were developed for broad use and are now important...

  15. Fluorescent Proteins: FRET

    Type
    Collection
    ...referred to as intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the...protein-protein interactions where each protein is separately fused to a donor or acceptor molecule (also referred...pairs with mTurquoise2 Mastop et al. (2017) Characterization of a spectrally diverse set of fluorescent...

  16. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...the image can be acquired more quickly. 2-Photon Microscopy 2-Photon Microscopy attempts to solve two ...Fluorescence microscopy. Nature methods 2, 910-919 (2005). PubMed PMID: 16299476. 2. Conchello JA, Lichtman JW. Optical...enhance spatial contrast (i.e. optical sectioning (2) or super-resolution (3). Fluorescence microscopy...photobleaching and a reduction in signal intensity. 2-Photon microscopes restrict excitation (and bleaching...can be seen exiting through the tissue. Therefore, 2-photon allows for deeper imaging into thick tissues...imaging objective that collects fluorescence. Like 2-photon microscopy, only a single focal plane of the...relying on raster scanning as is done in confocal or 2-photon microscopes. Total Internal Reflection Microscopy...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...repository of tools for SAR-CoV-2 research. Two new antibodies, Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17...Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86], target distinct epitopes on the SARS-CoV-2 nucelocapsid ...Anti-SARS-CoV-2 Antibodies here! Terry, J.S., et al. (2021). Development of a SARS-CoV-2 nucleocapsid ...prime editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector preps of Voltron2 voltage... incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7 shows enhanced editing efficiencies in a number...editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime editing. A) Schematic of PE7 prime...PMID: 38570691. Antibodies to facilitate SARS-CoV-2 research   By Ashley Waldron Ready-to-use recombinant...

  18. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...Suresh from Iowa State University. Since its appearance, SARS-CoV-2 has spread to almost every part of the ... C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature...Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity:10-...primers that target the E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences... method has been optimized to detect the SARS-CoV-2 virus directly using saliva without having to perform...and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter of RNA targets...single-molecule sensitive. In the case of HIV-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification was...

  19. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus may acquire mutations to escape ... SARS-CoV-2 infects host cells through binding the human angiotensin converting enzyme 2 (ACE2) cell-surface...Pooled library to study antibody escape in SARS-CoV-2 Recombinant antibodies for aminoacyl tRNA synthetases...viral preps Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple...viral spike protein.   Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing. From Starr... binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the...and a barcode, and is designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface...

  20. Neurodegeneration Research Collection

    Type
    Collection
    ...different inherited genes: Presenilin 1, Presenilin 2, and APP. The majority (> 90%) of individuals develop...Neurodegenerative diseases are a group of disorders characterized by the progressive degeneration and ultimate...biology of these diseases, rather than focusing on separate diseases, will help speed progress towards curing...
Showing: 41 - 60 of 492 results