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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 650 results for: CHI;

Showing: 41 - 60 of 650 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...gently touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well...nothing falls into the plate. Leave dilutions on the chilled 96-well freezer block in the BSC until ready to...Prepare the Master Mix Place a ddPCR plate onto a chilled 96-well freezer block and set aside in the droplet...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 20 µL of the master ...aspirating the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. In this protocol...

  2. CRISPR Library Amplification

    Type
    Protocol
    ...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to ...Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent...ice for 15-20 minutes or until completely thawed. Chill a box of 200 µL micropipette tips in a -20 °C freezer...kV, 1 pulse. Aliquot 25 µL DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation involves...sterile LB at 4 ℃. Day 2 (morning) Before beginning, prechill at least four conical tubes on ice and ensure ...

  3. Gibson Assembly Protocol

    Type
    Protocol
    ... order primers that are 60 bp long, with 30 bp matching the end of the adjacent fragment and 30 bp annealing...the assembled parts. Tips and Troubleshooting “Stitching” fragments together using oligos When you need...Pro-Tip Please note that the way to design the “stitching” primers and the amounts of primers to include...TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA...Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...doxycycline (Zhou et al., 2006). Tet-On 3G systems can achieve >10,000-fold induction of the gene of interest....cell lines or transgenic animals can be made to achieve greater control over expression with flexibility...Galla, M., Maetzig, T., Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral and transposon-based ...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ...sensitivity, wide dynamic range, and resistance to photobleaching has made luciferase a common choice in assays... of renilla luciferase Reuben Shaw 48688 pUltra-Chili-Luc Firefly Ubc Lentiviral expression of firefly... Mammalian expression of firefly luciferase Ming-Chih Lai 115366 p CIneo-RL Renilla CMV Mammalian expression...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ... gently touching the screen. Preparation of the Master Mix Place a ddPCR plate onto a chilled 96-well ...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 16 µL of the master ...pipetting the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the...

  7. Synthetic Biology - Overview

    Type
    Collection
    ...Resources iGEM - International Genetically Engineered Machine Foundation Registry of Standard Biological Parts...synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource EraSynBio...

  8. Chemogenetics AAV Preps

    Type
    Collection
    ...none 2, 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D(Gq) - Activation HA fusion none 2, 5 Richie 50467 pAAV-CaMKIIa-HA-hM4D...

  9. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013...

  10. Viral Vectors

    Type
    Collection
    ...scientific review of our viral pages, we thank Boston Children's Hospital Viral Core . Viral Plasmid Biosafety...Au S, Panté N. How viruses access the nucleus. Biochim Biophys Acta. 2011 Sep;1813(9):1634-45. doi: 10.1016...

  11. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...using streptavidin affinity purification. For both enCHIP and CAPTURE, design your gRNA sequence to direct...

  12. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below the primer...liquid to the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from...

  13. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...minutes at 95°C in a PCR machine or in a beaker of boiling water. If using a PCR machine, incubate the sample... that does not contain antibiotics. h. Without touching the sides of the dish, gently add DNA:FuGENE® ...PubMed . Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Zufferey R et...

  14. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...strategy relies on cellular delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double...of PCR products. Finney M, Nisson PE, Rashtchian A. Current Protocols in Molecular Biology. 2001...

  15. Bacterial Expression Systems

    Type
    Collection
    ...protein reporter genes in Gram-negative bacteria. Hochschild Bacterial Two-Hybrid System Various Plasmids ...with lambda-C1 operator Beta-galactosidase Ann Hochschild Bacterial two-hybrid assay using pBRα-β flap ...

  16. Plasmids for Stem Cell Research

    Type
    Collection
    ... Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...

  17. Biosensor AAV Preps

    Type
    Collection
    ...Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Patriarchi Oxytocin (OT...

  19. AAV Production in HEK293 Cells

    Type
    Protocol
    ...surface and should be handled carefully. Avoid touching the cells when replacing media. T-175 flask, Corning...plasmids, we can determine the total μg/bp we need to achieve a 1:1:1 molar ratio of each plasmid: 2000 μg / ...

  20. TALEN Engineering

    Type
    Collection
    ...assemble TAL effector repeat arrays on a single architectural framework and the DNAs produced by all three...
Showing: 41 - 60 of 650 results