...Browse Lenti Packaged on Request Can't find what you need in our in-stock preps? Our Packaged on Request service...plasmids and we’ll make the viral vector prep you need. Packaged on Request Viral Vector Packaging Service...

...spectrophotometry using a NanoDrop spectrophotometer. If needed, the sample is adjusted by diluting or concentrating... shipping conditions will be shown in your cart. Need Some Help? Browse some of our other resources to...

... an Account: Before placing your order, you will need to create an account and affiliate it with your ...has not ordered from Addgene in the past, we will need to verify your account. We will notify you by email...

...Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for..., preserves, reproduces, and distributes diverse seed and other stocks of Arabidopsis thaliana and related...

...rather than focusing on separate diseases, will help speed progress towards curing and treating them. Scientists... a lentiviral FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere et al. Mol Neurodegener...

...techniques, as well as the plasmids and protocols needed for using them, are described below. Read our CRISPR...gene of interest is currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9...

...editing at a given locus. To use the tool, you'll need Sanger sequencing reads from PCR amplicons that ... Program for designing optimal gRNAs. Provides feedback on number of potential off-targets, target's genomic...

...preparation. Systemic Delivery AAV Capsid Choice Need help deciding which capsid to choose? The following...plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what you would...

..., Tieu M, Trader C, van Velthoven CTJ, Walker M, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T,... S, Miller JA, Kalmbach BE, Radaelli C, Gore BB, Weed N, Omstead V, Bishaw Y, Shapovalova NV, Martinez...

...GST-FUS-WT FUS GST tac ALS Robin Reed 44981 GST-FUS 266-526 FUS GST tac ALS Robin Reed 44982 GST-FUS 356-526 FUS...FUS GST tac ALS Robin Reed 44983 GST-FUS 465-526 FUS GST tac ALS Robin Reed 44984 GST-FUS deltaNLS 1-...513 FUS GST tac ALS Robin Reed 44985 3X FLAG-FUS-WT FUS Flag CMV ALS Robin Reed 45640 pcDNA3 HA eIF4GI (...

... is introduced in the media. Generally, you will need two plasmids, as depicted in the figure below: A...changes to the standard translation machinery are needed in order to incorporate a non-canonical amino acid...

...from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA Viviana...tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTA Viviana...

...BsmBI none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none, need plasmid 65776...

...iGluSnFR4) for improved sensitivity and scale (4s) or speed (4f) of imaging synaptic transmission Glutamate ...to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage sensor to detect...

...techniques, and explain common applications. View Guide Need Some Help? Browse some of our other resources to...

...’re using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target...

...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target...

...kinds of important experiments. In an effort to speed up research of SARS-CoV-2, Ginkgo Bioworks (Link...

...type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA...

... to facilitate design of short oligonucleotides needed to construct gRNA expression vectors. More information...