...technology. Visit the Zhang Lab page and forum to learn more. CRISPR Design FAQs Homologous Recombination...the Cas9 nickase which will together introduce cleavage of the target site with efficiency similar to ... donor. Generally if off-target (non-specific) cleavage is not a very big concern, you could go ahead ...PX335 vector, you need to find two guides for each cleavage site, but this system probably have better specificity...sequence is required for successful targeting and cleavage, changing it to'NGT' or 'NGC', in addition to ...Cas9 and tracrRNA, it will be processed (i.e., cleaved) into a shorter form that contains 20bp of the ...mature tracrRNA), the spacer used is only 20bp. Please refer to Cong et al. , Science 2013 for more details...

...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted fluorescent...sensor (MARIO) A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically Encoded...3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad...sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin...

...cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form . Please note that this .... Cloning Grade DNA is an Addgene Beta service. Learn more about Addgene's Beta Projects and how you can...µl (± 5ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and check the individual...I find cloning resources or scientific support? Please see our Addgene Protocols for resources you may...

...Microtubules 87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20...114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP...NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding...fibrillar center) 133964 SON-mEGFP AICSDP-82 mEGFP SON Nuclear speckles 159744 DMD-mEGFP AICSDP-55 mEGFP Dystrophin...PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines...

...downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles...DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically...enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This...

...Constitutive 9 Wilson 105557 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG CB7 mCerulean Constitutive 1, 9 Wilson 105598 pAAV.GfaABC1D.PI.Lck-GFP.SV40...VCre-dependent Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...each viral lot. To learn which specific QC experiments were performed on your lot, please contact us . Full...medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation and then by filtration...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...the sample from the DNA tube all the way to the cleared viral preparation pool. Proviral Identity Confirmation...

...NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide...guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2 RASGRP3 RASGRP4 RAS guanyl releasing protein RASSF...activating pathways for cell growth and potentially leading to cancer. Constitutively active Ras is the most...

...pCS2plus-mCerulean 156-239-GGGS-TDP-43 TARDBP mCerulean CMV ALS Marco Morsch 162618 pCS2plus-mCerulean 156-...Parkinson's, FTD Michael Davidson 55431 mCerulean3-MAPTau-C-10 MAPT mCerulean3 CMV Parkinson's, FTD Michael Davidson...Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His, mCerulean3 T7 Parkinson's Dan Mulvihill 110486...Lippincott-Schwartz 164214 PLEX-PGK-ANXA11-mCerulean ANXA11 mCerulean PGK ALS Jennifer Lippincott-Schwartz ...mRuby CMV ALS Jennifer Lee 216227 TDP-43 mCerulean TARDBP mCerulean CMV ALS Jennifer Lee 216843 pET-Duet_...If you do not see a particular gene of interest, please search our full plasmid collection using the search...Bacterial Yeast Other Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease...

...Disease. These diseases persist for many years and lead to a major burden on the patients, their families.... Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using...spinal cord. Over time, a loss of motor neurons leads to limb weakness, difficulty speaking, swallowing...percentage (5-10%) are familial ALS cases having at least one other affected family member. These familial... individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It...

... and guides to help you use CRISPR in your lab. Learn More Guide to CRISPR technology Addgene Blog Posts...sequence) can be provided, to compare the predicted cleavage position to the position of the observed mutations... the Chan Zuckerberg Biohub . gRNA Design Deep learning models to design gRNAs and predict their efficiency...sequence or list of sequences. RGEN (RNA-guided endonuclease) Tools: Cas-OFFinder & Cas-Designer From the...Targeter Originally developed to identify zinc finger nuclease sites, this tool from the Joung Lab has been expanded...

...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and shared... Krüppel-like factors (KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse...collaborative efforts within the KLF community have lead to the establishment of this shared resource on ...encourage you to contribute to this community resource. Learn about the benefits of depositing plasmids with Addgene...

...downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed...DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically...enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This...

...chiRNAs can induce defined deletions between the two cleavage sites, while the addition of an ssODN donor template... of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue... 46294 pBS-Hsp70-Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.... 45945 pHsp70-Cas9 : The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter...

...Hochschild and colleagues ( Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et...this Kit These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite...

...Lab on the antibody webpage. To learn more about these antibodies, please visit the Institute for Protein...mammalian cells. To learn which specific QC experiments were performed on your lot, please email [email protected]...

...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

...Which Type of Virus Should I Use? Use this guide to learn more about the different types of viruses that are...select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit ...viruses are temporary in the host. Use this guide to learn more about the different types of viruses that are...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...

...Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV3508 98928...Wickersham AV-9-PV3248 105557-AAV9 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV1090 105537...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

...pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation... BioSciences (N153AA, C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN...