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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,017 results for: LEA

Showing: 41 - 60 of 1017 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...NUSA tag and TEV cleavage pExp-NusA - Expression of protein in E. coli as TEV cleavable N-terminal His ...of target gene with N-terminal GST tag and TEV cleavage Transient expression - Gradia Lab plasmids for...lab pFB-LIC-Bse - Insect expression vector from Gileadi lab Worm unc-54, variety of worm gene promoters...Guide and the Michael Davidson FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid...myc-nuc - pEntry vector that adds a C-terminal nuclear localization signal pGADCg - Gateway yeast expression...

  2. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Microtubules 87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20...114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP...NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding...fibrillar center) 133964 SON-mEGFP AICSDP-82 mEGFP SON Nuclear speckles 159744 DMD-mEGFP AICSDP-55 mEGFP Dystrophin...PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines...

  3. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles...DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically...enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This...

  4. Viral Production

    Type
    Collection
    ...each viral lot. To learn which specific QC experiments were performed on your lot, please contact us . Full...medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation and then by filtration...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...the sample from the DNA tube all the way to the cleared viral preparation pool. Proviral Identity Confirmation...

  5. Control AAV Preps

    Type
    Collection
    ...Constitutive 9 Wilson 105557 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG CB7 mCerulean Constitutive 1, 9 Wilson 105598 pAAV.GfaABC1D.PI.Lck-GFP.SV40...VCre-dependent Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  6. Ras Pathway

    Type
    Collection
    ...NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide...guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2 RASGRP3 RASGRP4 RAS guanyl releasing protein RASSF...activating pathways for cell growth and potentially leading to cancer. Constitutively active Ras is the most...

  7. Neurodegeneration Research Collection

    Type
    Collection
    ...Disease. These diseases persist for many years and lead to a major burden on the patients, their families.... Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using...spinal cord. Over time, a loss of motor neurons leads to limb weakness, difficulty speaking, swallowing...percentage (5-10%) are familial ALS cases having at least one other affected family member. These familial... individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It...

  8. KLF Research Plasmids

    Type
    Collection
    ...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and shared... Krüppel-like factors (KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse...collaborative efforts within the KLF community have lead to the establishment of this shared resource on ...encourage you to contribute to this community resource. Learn about the benefits of depositing plasmids with Addgene...

  9. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed...DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically...enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This...

  10. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form . Please note that this ... (± 5 ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and check the individual...I find cloning resources or scientific support? Please see our Addgene Protocols for resources you may...

  11. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Hochschild and colleagues ( Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et...this Kit These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite...

  12. Antibody Production

    Type
    Collection
    ...Lab on the antibody webpage. To learn more about these antibodies, please visit the Institute for Protein...mammalian cells. To learn which specific QC experiments were performed on your lot, please email [email protected]...

  13. AAV Molecular Tools

    Type
    Collection
    ...-pre-mGRASP-mCerulean CAG-driven, constitutive Expression of pre-synaptic mGRASP-mCerulean for synaptic...Genome Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  14. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCS2plus-mCerulean 156-239-GGGS-TDP-43 TARDBP mCerulean CMV ALS Marco Morsch 162618 pCS2plus-mCerulean 156-...Parkinson's, FTD Michael Davidson 55431 mCerulean3-MAPTau-C-10 MAPT mCerulean3 CMV Parkinson's, FTD Michael Davidson...Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His, mCerulean3 T7 Parkinson's Dan Mulvihill 110486...Lippincott-Schwartz 164214 PLEX-PGK-ANXA11-mCerulean ANXA11 mCerulean PGK ALS Jennifer Lippincott-Schwartz ...mRuby CMV ALS Jennifer Lee 216227 TDP-43 mCerulean TARDBP mCerulean CMV ALS Jennifer Lee 216709 pCAG-TSF-...If you do not see a particular gene of interest, please search our full plasmid collection using the search...Bacterial Yeast Other Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease...

  16. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV3508 98928...Wickersham AV-9-PV3248 105557-AAV9 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV1090 105537...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ...pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation... BioSciences (N153AA, C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. Its robust signal, ...sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular tool for scientists...making it ideal for a variety of applications. To learn more and plan your experiment, check the plasmid's...Artificial luciferases desined de novo using deep-learning approaches. Split Luciferase Complementation assays...

  19. Caltech Systemic Capsids

    Type
    Collection
    ...mice use LY6A to cross the blood-brain barrier, leading to strain-specific tropism (See S3 table (Huang...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER...using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  20. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...modify a single genomic locus. Before ordering, please review the steps for using pooled CRISPR libraries...Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species PI Lentiviral...Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...
Showing: 41 - 60 of 1017 results