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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 497 results for: RAP

Showing: 41 - 60 of 497 results

  1. NETRF

    Type
    Collection
    ... that will help create new and more effective therapies for these tumors. The NETRF encourages others ...

  5. TALEN Engineering

    Type
    Collection
    ...sites and then generates individual user-friendly graphical roadmaps for each target that guide the user through...

  6. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...poorly understood but are critical for developing therapeutic strategies to prevent viral spread. As a Biosafety...

  7. Retrovirus Plasmids

    Type
    Collection
    ...deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused...

  8. Antibody Production

    Type
    Collection
    ... columns. Formulation Buffer After affinity chromatography, antibodies undergo a buffer exchange using...

  10. AAVED

    Type
    Collection
    ...Resources Missing in the Field Kenton Woodard 4:00 PM Wrap Up & Next Steps Melina Fan/Leila Haery 5:00 PM Adjourn...

  11. Viral Production

    Type
    Collection
    ...than full vector particles. Figure 3: Electron micrograph of AAV vector preparation shows that the vast...

  12. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...Fisher 21028 Protein G GraviTrap columns, Cytiva 28-9852-55 or rProtein A GraviTrap columns, Cytiva 28-9852...and mix well. Uncap the Protein G Gravitrap or rProtein A Gravitrap columns and gently pour off the ethanol...Buffer Reservoirs to the top of the Gravitrap column. Pro-Tip Gravitrap columns have a finite binding capacity...the Gravitrap column to a clamp on a clamp stand. Use scissors to cut the bottom of the Gravitrap column...the Gravitrap columns at the bottom. Add 5 mL of Pierce IgG Elution Buffer to the capped Gravitrap column...430281 0.45 µm PES complete filtration unit, 500 mL, rapid-flow, VWR 73520-984 Syringes, 30 mL, VWR BD302832...upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate filtered tissue culture supernatant...

  13. Brain Armamentarium

    Type
    Collection
    ...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru and Chad Cowan Ekker...

  15. CRISPR Library Amplification

    Type
    Protocol
    ...during the scraping process. Add each scrape into a 50 mL conical tube on ice pooling the scrapings of two...spreader and cold LB to scrape bioassay plates and remove bacteria. Use one scraper for all plates. Use two...mL cold LB to each plate for each scrape and use spreader to scrape plates. Pro-Tip Pushing motion is ... each tube on ice while scraping. Repeat addition of 10 mL cold LB and scrape for each plate until agar...spreading. Some spreaders have a sharp edge that can scrape plates more abrasively at a certain angle. Incubate...conical tubes on ice and ensure access to sterile scraper and cold LB. Pro-Tip Prepare one to two more conical...removing LB containing bacteria from plate). Pro-Tip Scraped bacteria in LB can clog the pipette either mix ...

  17. Caltech Systemic Capsids

    Type
    Collection
    ...for the AAV Decision Tree The AAV decision tree graphic above helps you choose the best capsid for your...

  18. The Pleiades Promoter Project

    Type
    Collection
    ...window) was established to advance research and therapies for brain disorders by enabling region- and cell-specific...

  19. Bacterial Expression Systems

    Type
    Collection
    ...from the culture and purified through affinity chromatography. To facilitate the purification process, proteins...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...) system is one of the most popular choices for rapid, efficient, and reversible control of gene expression...
Showing: 41 - 60 of 497 results