...tracking of viral transduction. See article for more similar plasmids. Elledge 21373 pHIV-EGFP 3rd EF-1alpha...

...transcriptional activation domain from HSV (herpes simplex virus). In the absence of tetracycline, the tetR...

...top General Plasmid Tools SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably...

...introduce cleavage of the target site with efficiency similar to using a single chimeric gRNA. At the same time...

...pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with simultaneous dTomato expression Recombinases Larsen 107788...

... Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM. A regulatory toolbox of MiniPromoters to drive...

...., Rodriguez-Rozada, S., Dieter, A., Pieper, F., Sims, R., Tenedini, F., Bergs, A. C. F., Bendifallah,...cascade of the intracellular part of the receptor, similar to what the endogenous binding partner would do...over when and where the receptor is activated.  Similarly, PTLs are special, covalently linked molecules... light exposure to activate them. PORTLs use a similar concept, except the ligand is attached to a protein...

...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...

...non-specific labeling observed Publications Ben-Simon Y, Hooper M, Narayan S, Daigle T, Dwivedi D, Way...

...Super-resolution techniques such as Structured Illumination (SIM), Single Molecule Localization (PALM/STORM), or Reversible... the world around us. Without contrast, objects simply vanish into noise. Due to its unrivaled contrast...Fluorescent Widefield Microscopy is the most common and simplest form of fluorescent microscopy. Collimated (non-converging...thicker three dimensional sample (see Figure 1); similar to an MRI or CT scanner. This is accomplished by...penetrates deeper into biological tissue. To prove this, simply hold a flash light to the palm of your hand. Although... excited at one time, limiting photobleaching. Similar to widefield, the entire field of view is excited...Website Other major microscopy manufacturers have similar information References 1. Lichtman JW, Conchello...

...ImageJ or similar software Reagents Purified recombinant antibody, 0.9–1.1 mg/mL SimplyBlue SafeStain,... bio-safe Coomassie G-250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample is...and reagents from other vendors should produce similar outcomes when using these protocols. However, please... Pour off the water in the sink. Add 20 mL of SimplyBlue SafeStain and incubate for 1 h with gentle agitation...agitation on a rocking platform. Pour off the SimplyBlue SafeStain in the sink. Add 100 mL of deionized...Analysis Use ImageJ (Link opens in a new window) or a similar photo software to determine the relative intensity...

...cloning sites, will result in similar sized bands, thus making this simple digest less informative. This...Plasmid Size -OR- Insert and Backbone Size The simplest form of diagnostic digest is one in which you ...products from each orientation are different sizes. Simply run the digest products on a gel and those clones...

...choice with minimal limitations. Background In its simplest form, PCR based cloning is about making a copy...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get... get PCR amplification. We can start similarly, taking the final 18bases of the ORF, including the stop...primer. If you are amplifying from a plasmid or simple template, there is very little chance for mis-priming...

... (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol® or similar product such as TRI Reagent®, RNAzol®, QIAzol® ...how effective your cell lysis protocol is. While simple homogenization is effective for most mammalian ...Homogenize or lyse tissues or cells in TRIzol® or a similar product. For tissues: use 1 mL of TRIzol® per 100...

...bands needed to be separated - see FAQs below . Simply adjust the mass of agarose in a given volume to...How do you get better resolution of bands? A few simple ways to increase the resolution (crispness) of ...you get better separation of bands? If you have similarly sized bands that are running too close together...

...primers are mainly used for amplifying a small, simple fragment of DNA. On the other hand, a long primer...The structure of the primer should be relatively simple and contain no internal secondary structure to ...

...do you get better resolution of bands? A couple simple ways to increase the resolution (crispness) of ...you get better separation of bands? If you have similarly sized bands that are running too close together...

... Your Primers Primer design for LIC is often as simple as using the backbone manufacturer's suggested ...the first G on 3'-5' strand from cut site). For simplicity, only the 5' primer is shown here. Note: Use ...

...RNA extractions. Plus, centrifuges are useful for simply collecting liquid to the bottom of a tube. You ... can be about bench height. They often spin at similar speeds to their smaller cousins, but can hold much...

... al. , 2010). Number of fragments assembled simultaneously Multiple fragments can be assembled in one ...opens in a new window) Rabe BA, Cepko C. (2020). A Simple Enhancement for Gibson Isothermal Assembly. bioRxiv...