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Showing: 41 - 60 of 796 results

  1. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ... heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be ...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 ...

  2. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... of detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like...imaging has numerous advantages over other imaging techniques, including that it is easy to implement due to...FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics in living cells. The fluorescent...

  3. Zhang Lab CRISPR Page

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    ...trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system using two...express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014). Full references are below. Available ...mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements...Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647...Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055...

  4. Bacterial Expression Systems

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    ...bacterial genomes for your research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini... expressed in vivo . Plasmid ID Visualization Technique/Tag PI Purpose Empty Backbones with Fluorescent...Reporter Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium....small molecule, the reporter plasmid can be used to detect the presence of that small molecule and sometimes...) in the plasmid. Other similar reporters for detecting maltose can be found here . pAra Series Various...

  5. Zhang Lab's CRISPR Frequently Asked Questions

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    ...assembled a list of FAQs about using the lab's CRISPR technology....assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn ...addition to mutations in the spacer itself, will protect the HR template from the Cas9. Again, if it's within...of the Cong, et. al, 2013 Science paper). If you detect positive HR, isolate single-cell colonies, grow...

  6. Genomic Deletions in Mammalian Cell Lines

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    ...of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization...bp from the predicted cleavage site to ensure detection would not be impacted by a small indel at the ...in Molecular Biology. 2001. PubMed . MicroRNA detection in prostate tumors by quantitative real-time PCR...PubMed . Western blotting: sample preparation to detection. Eslami A, Lujan J. J. Vis. Exp . 2010...

  7. Luciferase Plasmid Collection

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    ...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent...more about pHlash sensor in our 28 Hot Plasmid Technologies from 2015 blog post. RNA Biology CMV-LUC2CP/...investigating regions controlling transcription Pete Stecha 212933 pGL4.84(hRlucCP/Puro) Renilla Vector for...investigating regions controlling transcription Pete Stecha Return to Top Expression Constructs Luciferase ...

  8. AAV Viral Preps

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    ...can be safely handled at the lowest biosafety protection levels. High quality and high titer in-stock ...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1...serotypes AAV1, AAV2, AAV5, AAV8, AAV9, AAVrg, the Caltech systemic serotypes, or others, it will be listed...

  9. Institute for Protein Innovation

    Type
    Collection
    ...opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...collection includes anti-glypicans that accurately detect glypican presence and explore glypican function...that recognize common epitope tags to help purify, detect, or localize particular proteins. Browse or search...

  10. New England Biolabs Cell-Imaging Plasmid Collection

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    ...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...

  11. Antibody Plasmid Collection

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    Collection
    ...proteins, visualize a protein under a microscope, or detect when the protein is present in a sample--just to...Vector system for expression, dimerisation and detection of recombinant antibody fragments in the form ...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system...

  12. Plant Plasmids and Resources

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    ...spanning a wide variety of molecular biology technologies: from genome engineering and gene regulation...CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR Resources for a full selection...initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement ...

  13. Zebrafish Plasmid Collection

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ...

  14. Fujii Lab CRISPR Plasmids

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    ...2009 (Hoshino & Fujii), the locus-specific ChIP technologies enable us to purify specific genomic regions...interacting with those regions. Locus-specific ChIP technologies consist of insertional chromatin immunoprecipitation...DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins. ...

  15. TALEN Plasmids and Kits

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    Collection
    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

  16. Tetracycline Inducible Expression

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    ...Krueger C, Pfleiderer K, Hillen W, Berens C. Biotechniques . 2004 Oct;37(4):546, 548, 550. PubMed . Optimization... R, Heinz N, Hampf M, Bujard H, Gossen M. BMC Biotechnol . 2010 Nov 24;10:81. doi: 10.1186/1472-6750-10...-10-81. PubMed . Tet Systems: Science and Technology (Link opens in a new window) Do you have suggestions...

  17. Rett Syndrome

    Type
    Collection
    ... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

  18. Zinc Finger Consortium Reagents

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    ...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

  19. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of...

  20. Antibodies

    Type
    Collection
    ...you choose and use antibodies, with an expert technical support team ready to answer your questions. All... antibody production and storage techniques, and explain common applications. View Guide ...
Showing: 41 - 60 of 796 results