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We narrowed to 100 results for: dcas9, grna

Showing: 41 - 60 of 100 results

  1. Editor's Choice, August 2016

    Type
    Blog Post
    ...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...CRISPRi uses a nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription machinery...loss (Ho et al. 2014). To overcome this, paired gRNAs can be used to target specific sizes of a DNA fragment..., removing large lncRNA fragments with multiple gRNAs poses the risk of disrupting adjacent genes, especially...Cas12a variant can cleave single-stranded RNA using gRNAs to specifically edit or degrade targeted lncRNAs...around 20 base pairs long. Using a single guide RNA (sgRNA) to create a double-strand break works well because...locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands of ...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology ...TurboCas provides the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, ...proximity ligation tool (Cenik et al., 2024). While dCas9 has been used in combination with proximity labeling...background labeling. By fusing miniTurboID with dCas9 to create TurboCas, the Shilatifard Lab created ...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...the gene into a gRNA finder such as WU-CRISPR (Wong et al., 2015)) or our lab’s sgRNA scorer 1.0 (Now ...environmental triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to specific...uses for Cas9 activators is in genetic screening. gRNAs targeting every gene in the human genome for example...targeted by the activator by simply providing a new gRNA rather than engineering an entirely new protein....difficult to construct and deliver when compared to gRNAs. Additionaly, cDNAs cannot be used to study in cis...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single gRNA expression. Although... target RNA without cleaving the molecule (like dCas9) suggests that dCas13a may be useful for isolating....org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check Out Our CRISPR Guide...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...nickase) fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus A target base ...coupling a cytidine deaminase with the inactive dCas9 (Komor et al., 2016). These fusions convert cytosine...an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change.... To do so, they used a Cas nickase, instead of dCas9. The resulting editor, BE3, nicks the unmodified...CRISPR plasmids for base editing Find validated gRNAs ...

  7. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter...

  8. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding known ...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...

  9. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding known ...

  12. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or ... Biotin tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing BirA...CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known ...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying molecules...of any genomic sequence specified by a particular gRNA. In the enChIP (engineered DNA-binding molecule-...or gRNA for efficient purification. Various epitope tags including 3xFLAG-tag, PA, and biotin tags, can...

  13. CRISPR Plasmids - Plants

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...

  14. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR dCas9-FokI CRISPR: dCas9-FokI...Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI...specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...

  15. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor ...expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter/enhancer...

  16. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...

  17. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9 fused to a transcriptional...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

  18. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...

  19. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX)... used this technology, which they call C-BERST (dCas9–APEX2 biotinylation at genomic elements by restricted...This ‘Caspex’ construct is guided by a pre-defined sgRNA sequence to a specific genomic locus and labels ... and targeted to a sequence of interest with an sgRNA expressed from a separate plasmid. When cells are...

  20. CRISPR Guide

    Type
    Collection
    ... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent... specified by a particular gRNA. Epitope tag(s) are fused to dCas9 or gRNA for efficient purification ...CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator with a gRNA to increase transcription of a target...CRISPRi CRISPR Interference; using a dCas9 or dCas9-repressor with a gRNA to repress/decrease transcription... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...
Showing: 41 - 60 of 100 results