We narrowed to 437 results for: ena

1. AAV Production in HEK293 Cells

   Type

   Protocol

   ...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence...

2. Lentivirus ddPCR Titration

   Type

   Protocol

   ...°C/sec) # Cycles Enzyme Activation 95 10 2 1 Denaturation 94 0.5 2 40 Annealing/Extension 60 1 2 40 Enzyme...

3. Cre-lox system

   Type

   Collection

   ...Cre: The fusion of Cre to a fluorescent reporter enables visualization of Cre expression. Optimized Cre:...expression when cloning in E. coli . This alteration enables the generation of a single vector containing Cre...

4. Depositor Collections

   Type

   Collection

   ...Expression Cell Migration Consortium Malate Dehydrogenase CUREs Community (MCC) Collection Pleiades Promoter...

5. Ginkgo Bioworks COVID-19 Collection

   Type

   Collection

   ...Department of Bioengineering, to build DNA that will enable researchers to express SARS-CoV-2 viral proteins...

6. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

   Type

   Collection

   ...modified their web-based ZiFiT Targeter software to enable identification of potential RGN target sites and...

7. Cancer Research Plasmids and Resources

   Type

   Collection

   ...signaling and physiology, and, when dysregulated, can enable cancer cell survival. Although cancers are partially...

8. CRISPR Plasmids - Purify Genomic Loci

   Type

   Collection

   ...purification methods are more straightforward and enable direct identification of molecules associated with...

9. CRISPR Plasmids - Parasites

   Type

   Collection

   ...of these parasites. Together, these tools have enabled precise genetic analyses to pinpoint genes linked...

10. Bikard Lab - CRISPR Repression Collection

    Type

    Collection

    ... Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial genes....

11. AAV for Neuronal Tracing

    Type

    Collection

    ...virus cannot spread to any neighboring cells. To enable monosynaptic spread of this deletion-mutant rabies...

12. Antibody Production

    Type

    Collection

    ...Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside serial dilutions of a ...

13. mTOR Pathway

    Type

    Collection

    ...rapamycin p53 TP53; tumor protein p53 PDK1 Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA...

14. Adeno-associated virus (AAV) Plasmids

    Type

    Collection

    ...circumvented with “helper-virus free systems,” which enable the production of infectious AAV particles without...

15. Microbiology Resources

    Type

    Collection

    ...Sulfolobus islandicus Plasmids for Bacteria Species Anabaena sp. Bacillus sp. Burkholderia sp. Bordetella pertussis...

16. Worm Expression Resources

    Type

    Collection

    ...cycle (3 days from egg to adult) and easy lab maintenance. Worms are particularly well suited for studies...

17. Open Enzyme Collection

    Type

    Collection

    ...biochemistry, and more. The foundational technologies enabled by this collection include polymerase chain reaction...

18. Rinehart Lab Phosphoprotein Reagents

    Type

    Collection

    ...encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent protein-peptide...

19. COVID-19 Resources

    Type

    Collection

    ...for utilizing SHERLOCK to detect coronavirus - Enabling coronavirus detection using CRISPR-Cas13: Open-access...

20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type

    Collection

    ...have an antibody against it. Another common scenario is fusing your protein to another protein, such...