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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 363 results for: gne

Showing: 41 - 60 of 363 results

  1. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in mammalian systems....Expression The following CRISPR plasmids have been designed for use in mammalian expression systems. Cut Fully...

  2. CRISPR Plasmids - Plants

    Type
    Collection
    ...CRISPR expression plasmids that have been designed for use in plants.... Posts The following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR...

  4. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...most yeast vectors and many mammalian vectors are designed to shuttle between species. Figure 1: Shuttle...host’s replication machinery. A shuttle vector designed for unrelated bacteria should include oris for...target species. Fun fact! Plant vectors are usually designed to shuttle between E. coli and Agrobacterium tumefaciens...image for individual analysis. A shuttle vector designed for this approach in a mammalian cell experiment...

  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...reproducibility than others. Unfortunately, most ELNs are designed as closed ecosystems and act as data silos because...RSpace ELN supports reproducibility RSpace was designed in response to requirements that emerged from ... in institutional and lab servers specifically designed to hold large amounts of data. Although it may...Since most ELNs, unlike data repositories, are designed to support confidentiality and limit permissions...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    ...utilizing chemogenetics (Designer Receptors Exclusively Activated By Designer Drugs, DREADDs) and optogenetics...examining the use of DREADDs (Designer Receptors Exclusively Activated By Designer Drugs) to determine the ...

  7. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...many MoClo kits are designed with specific model organisms in mind, others are designed for flexibility. .... phaffii, then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al... the Multi-Kingdom (MK) Golden Gate system was designed to work in fungi, bacteria, protists, plants, ...

  8. CRISPR References and Information

    Type
    Collection
    ...organisms. Developed by the Jin-Soo Kim Lab . Cas-Designer (Link opens in a new window) Searches for targets...a a low probability of off-target effects. Cas-Designer integrates information from Cas-OFFinder and Microhomology...

  9. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal activity with a magnetic field. Listen...Listen to Our Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's...

  10. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    .... “When PhD students graduate there has to be champagne, that's a given.” Furthermore, anyone leaving ...fellowships, jobs, or paper submissions with dinner and champagne in the lab. The Church Lab celebrates successful...toasts a newly minted PhD with food, cake, and champagne. What does your lab do for fun as a group? The...

  11. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... them. For the application we were looking at, magnetic isolation of circulating tumor cells, we found...may have an impact on this challenge either for magnetic capture or for capture using microfluidic devices...peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Fierer et al (Proc Natl Acad...

  13. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...why each is important. Our “Plasmids 101” series designed to educate all levels of scientists and plasmid...basics and spend more time developing cleverly designed experiments and innovative solutions necessary...utilized in the lab are usually artificial and designed to introduce foreign DNA into another cell. Minimally...

  14. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ..., IDT scientists Mollie Schubert and Shuqi Yan designed experiments to test nickase preferences using ...between the nicks? To find out, Schubert and Yan designed a PAM-out nickase experiment to insert an EcoRI...that are further away from the target site, they designed a D10A nickase strategy (46 nt nick distance, ...

  15. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...verification experiments that can be partially designed using the Snapgene desktop software. If a plasmid...verify the sequence within that region. We have designed many primers for Sanger sequencing at Addgene....function, we can detect all of the primers we have designed that will anneal to the plasmid sequence. We can...

  16. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...multiple fragments of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA...purification steps and end-repair steps with well-designed fragment overlap sections, while still creating... individual fragments containing mutations and aligned via regions of homology. With NEBuilder HiFi DNA...

  17. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...or performance review. You can’t delegate tasks assigned to you and you must usually take on all tasks ... be able to communicate these well to the task assignee and this is a recipe for bad outcomes. Clearly...little, but be careful not to go too far. Is the assignee interested in this task? Don’t give out tasks ...

  18. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein...brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will work better with shorter...

  19. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ... Addgene guest blogger post about colony PCR, I signed up immediately. I had used colony PCR before and...this method. I enjoyed writing the first post so I signed up to write a second. While working on this post...use Twitter as a scientist. While this post is designed with beginners in mind, there’s some great reminders...

  20. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...ng gDNA, and H 2 O up to 20 μl. Use the primers designed in step 2 above. Conduct PCR for “non-deletion.... Optimize PCR conditions for each primer pair designed based on testing the bulk sorted cells. Run samples...
Showing: 41 - 60 of 363 results