Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 368 results for: gne

Showing: 41 - 60 of 368 results

  1. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    .... “When PhD students graduate there has to be champagne, that's a given.” Furthermore, anyone leaving ...fellowships, jobs, or paper submissions with dinner and champagne in the lab. The Church Lab celebrates successful...toasts a newly minted PhD with food, cake, and champagne. What does your lab do for fun as a group? The...

  2. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... them. For the application we were looking at, magnetic isolation of circulating tumor cells, we found...may have an impact on this challenge either for magnetic capture or for capture using microfluidic devices...peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Fierer et al (Proc Natl Acad...

  3. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in C. elegans.... Posts The following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR...

  4. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ..., IDT scientists Mollie Schubert and Shuqi Yan designed experiments to test nickase preferences using ...between the nicks? To find out, Schubert and Yan designed a PAM-out nickase experiment to insert an EcoRI...that are further away from the target site, they designed a D10A nickase strategy (46 nt nick distance, ...

  6. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...why each is important. Our “Plasmids 101” series designed to educate all levels of scientists and plasmid...basics and spend more time developing cleverly designed experiments and innovative solutions necessary...utilized in the lab are usually artificial and designed to introduce foreign DNA into another cell. Minimally...

  7. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...verification experiments that can be partially designed using the Snapgene desktop software. If a plasmid...verify the sequence within that region. We have designed many primers for Sanger sequencing at Addgene....function, we can detect all of the primers we have designed that will anneal to the plasmid sequence. We can...

  8. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...multiple fragments of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA...purification steps and end-repair steps with well-designed fragment overlap sections, while still creating... individual fragments containing mutations and aligned via regions of homology. With NEBuilder HiFi DNA...

  9. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...or performance review. You can’t delegate tasks assigned to you and you must usually take on all tasks ... be able to communicate these well to the task assignee and this is a recipe for bad outcomes. Clearly...little, but be careful not to go too far. Is the assignee interested in this task? Don’t give out tasks ...

  10. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein...brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will work better with shorter...

  11. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in Drosophila and other insects.... Posts The following CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully...

  12. CRISPR Plasmids - Yeast

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in yeast.... Posts The following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/...

  13. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ... Addgene guest blogger post about colony PCR, I signed up immediately. I had used colony PCR before and...this method. I enjoyed writing the first post so I signed up to write a second. While working on this post...use Twitter as a scientist. While this post is designed with beginners in mind, there’s some great reminders...

  14. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs... at 3 days post fertilization. Since Ma et al. designed a repair template with two synonymous mutations...thus quieting some fears about the creation of “designer babies.” The differences in editing between iPS...

  15. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...machine-learning model to help them construct “designer” channelrhodopsins with desired characteristics...experiments. The sequences in this library were designed using structural information about parental channelrhodopsins...existing channelrhodopsins CoChR and/or ChR2(H134R). Designer channelrhodopsins paired with a systemic AAV allow...

  16. Promega Plasmid Collection

    Type
    Collection
    ...contains plasmids developed by Promega scientists and designed to streamline molecular and cellular biology experimental... a new window) is an energy transfer technique designed to measure molecular proximity in living cells...

  17. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in bacteria....Bacteria The following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/...

  18. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...ability to correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization of assembly systems...methodology. To further push the test system, we redesigned for 12 and 24 fragments.  This redesign was guided...fragment versions of the lacI/lacZ cassette were designed and synthesized. In conjunction with BsaI-HFv2...

  19. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...We should note that these vectors are primarily designed for bicistronic expression; however, many could...the table above (and their related plasmids) are designed to have one or more of the genes replaced.  Additionally...save on your desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...biorender.com   Another method of harvesting uses magnetic beads. In this method, beads are incubated with...suspension. Antibodies bind ligands on the beads and a magnet is used to separate the beads from the media (Brechmann... Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension. ...
Showing: 41 - 60 of 368 results