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We narrowed to 794 results for: tec

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Showing: 41 - 60 of 794 results

  1. COVID-19 Resources

    Type
    Collection
    ...For rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics: DETECTR (Link opens...tools, like Cas12a and Cas13a plasmids, to reliably detect nucleic acids SARS-CoV-2 main protease (Mpro) activity...protease, and polymerase constructs for SARS-CoV-2 detection assays Neutralizing nanobody plasmids And more...Addgene blog Finding nucleic acids with SHERLOCK and DETECTR No Llamas Required - Synthetic Nanobodies Against...

  2. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ... heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be ...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 ...

  3. CRISPR Plasmids for Genomic Visualization

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    ... of detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like...imaging has numerous advantages over other imaging techniques, including that it is easy to implement due to...FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics in living cells. The fluorescent...

  4. Zhang Lab CRISPR Page

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    Collection
    ...trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system using two...express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014). Full references are below. Available ...mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements...Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647...Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055...

  5. Genetic Code Expansion

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    ...99222 pTECH-chPylRS(IPYE) PylRS Chimeric Nε-boc-lysine (BocK) Bacterial TAG Dieter Söll 104069 pTECH-chAcK3RS...104070 pTECH-MbAcK3RS(IPYE) AcK3RS M. barkeri Nε-acetyl-L-lysine Bacterial TAG David Liu 104071 pTECH-MmAcK3RS...104072 pTECH-MbPylRS(IPYE) PylRS M. barkeri Nε-boc-lysine (BocK) Bacterial TAG David Liu 104073 pTECH-MmPylRS...

  6. Rinehart Lab Phosphoprotein Reagents

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    ...popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus plasmid...split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent protein-peptide interactions...kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library ...phosphorylation-dependent interactions . Nature Biotechnology , 36 (7), 638–644. https://doi.org/10.1038/nbt...

  7. Tetracycline Inducible Expression

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    ...binding of Tet-Advanced transactivator (developed by Clontech, based on Resnitzky et al., 1994). TRE3G promoter...alternative effectors for Tet transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04...promoters with minimized background expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10...opens in a new window) Tet Systems: Science and Technology (Link opens in a new window) Content last reviewed...

  8. AAV Viral Preps

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    ...can be safely handled at the lowest biosafety protection levels. High quality and high titer in-stock ...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1...AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed...

  9. Plan Your Experiment

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    ...editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID:...more information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different... analysis of CRISPR–Cas experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587...

  10. New England Biolabs Cell-Imaging Plasmid Collection

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    ...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...proteins, visualize a protein under a microscope, or detect when the protein is present in a sample — just ...Vector system for expression, dimerisation and detection of recombinant antibody fragments in the form ...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system...

  12. Distribution to Industry

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    Collection
    ... Proteins Mikel Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. Protein...intellectual property rights, and provides liability protection for material providers. Addgene's deposit agreements...distribution. Please see the Sample Agreements on our Technology Transfer Information page for more information...

  13. Zebrafish Plasmid Collection

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    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ...

  14. Plant Plasmids and Resources

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    ...spanning a wide variety of molecular biology technologies: from genome engineering and gene expression...luminescent, and color-producing proteins for detection of gene expression, protein localization, protein–protein...initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement ...

  15. TALEN Plasmids and Kits

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    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

  16. Rett Syndrome

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    ... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

  17. Luciferase Plasmid Collection

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    ... luciferases require different substrates and detection wavelengths. Firefly luciferase is brighter than...investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating...investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5'...

  18. Zinc Finger Consortium Reagents

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    ...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

  19. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of...

  20. Antibodies

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    ...you choose and use antibodies, with an expert technical support team ready to answer your questions. All... antibody production and storage techniques, and explain common applications. View Guide ...
Showing: 41 - 60 of 794 results