Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 799 results for: tec

Showing: 41 - 60 of 799 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...capacity to give rise to an entire organism. iPSC technology removes the possible ethical concerns related...tumorigenicity, an inherent disadvantage of iPS cell technology. Browse Addgene's collection of Transdifferentiation...Transdifferentiation Plasmids below. Many of the techniques for reprogramming, differentiation, and transdifferentiation... lineage conversion of acinar cells. Nature Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic...oligodendroglial cells by direct lineage conversion. Nat Biotechnol. 2013 May;31(5):434-9. Wernig Fibroblasts Oligodendrocyte...myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol. 2013 May;31(5):426-33. Tesar Fibroblasts Medium...

  2. Viral Production

    Type
    Collection
    ...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre. ... are determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity.... mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (...for mycoplasma contamination using mycoplasma detection kits. The cell line is maintained for ~20 passages...

  3. Genetic Code Expansion

    Type
    Collection
    ...104069 pTECH-chAcK3RS(IPYE) AcK3RS Chimeric Nε-acetyl-L-lysine Bacterial TAG David Liu 104070 pTECH-MbAcK3RS...104071 pTECH-MmAcK3RS(IPYE) AcK3RS M. mazei Nε-acetyl-L-lysine Bacterial TAG David Liu 104072 pTECH-MbPylRS...M. mazei Cyanobacterial TAG Lital Alfonta 99222 pTECH-chPylRS(IPYE) PylRS Chimeric p-iodo-L-phenylalanine...-L-phenylalanine Bacterial TAG David Liu 104073 pTECH-MmPylRS(IPYE) PylRS M. mazei m-iodo-L-phenylalanine...

  4. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ... heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be ...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 ...

  5. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... of detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like...imaging has numerous advantages over other imaging techniques, including that it is easy to implement due to...FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics in living cells. The fluorescent...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system using two...express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014). Full references are below. Available ...mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements...Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647...Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent... luciferases require different substrates and detection wavelengths. Firefly luciferase is brighter than...investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating...investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5'...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization...bp from the predicted cleavage site to ensure detection would not be impacted by a small indel at the ...in Molecular Biology. 2001. PubMed . MicroRNA detection in prostate tumors by quantitative real-time PCR...PubMed . Western blotting: sample preparation to detection. Eslami A, Lujan J. J. Vis. Exp . 2010...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...binding of Tet-Advanced transactivator (developed by Clontech, based on Resnitzky et al., 1994). TRE3G promoter...alternative effectors for Tet transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04...promoters with minimized background expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10...opens in a new window) Tet Systems: Science and Technology (Link opens in a new window) Do you have suggestions...

  10. AAV Viral Preps

    Type
    Collection
    ...can be safely handled at the lowest biosafety protection levels. High quality and high titer in-stock ...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1...AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed...

  11. Institute for Protein Innovation

    Type
    Collection
    ...opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...collection includes anti-glypicans that accurately detect glypican presence and explore glypican function...that recognize common epitope tags to help purify, detect, or localize particular proteins. Browse or search...

  12. Plan Your Experiment

    Type
    Collection
    ...editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID:...more information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different... analysis of CRISPR–Cas experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587...

  13. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...

  14. Antibody Plasmid Collection

    Type
    Collection
    ...proteins, visualize a protein under a microscope, or detect when the protein is present in a sample--just to...Vector system for expression, dimerisation and detection of recombinant antibody fragments in the form ...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system...

  15. Distribution to Industry

    Type
    Collection
    ... Proteins Mikel Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. Protein...intellectual property rights, and provides liability protection for material providers. Addgene's deposit agreements...distribution. Please see the Sample Agreements on our Technology Transfer Information page for more information...

  16. Plant Plasmids and Resources

    Type
    Collection
    ...spanning a wide variety of molecular biology technologies: from genome engineering and gene regulation...CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR Resources for a full selection...initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement ...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ...

  18. TALEN Plasmids and Kits

    Type
    Collection
    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

  19. Rett Syndrome

    Type
    Collection
    ... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

  20. Zinc Finger Consortium Reagents

    Type
    Collection
    ...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...
Showing: 41 - 60 of 799 results