We narrowed to 601 results for: Ret;
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TypeCollection...for the AAV1, AAV5, AAV6, AAV8, AAV11, or AAV Retrograde serotypes and 2 x 10 12 GC/mL for the AAV2 serotype...
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Synthetic Biology - Overview
TypeCollection...Modular PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. ... -
Antibody Production
TypeCollection... ensure that potency and specificity have been retained. If the target antigen is not endogenously-expressed... -
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...-surface proteins, and are useful for labeling secreted proteins with disulfide bridges such as antibodies... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...https://doi.org/10.1128/mbio.00019-20 Singh AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post... of ~50% and confirmed that the fusion protein retained the PAM properties of the individual Cas9. They... -
Arf GTPase Family
TypeCollection...Mammalian (pEGFP-C1) GEF Fbxo8 (FBX8) 26269 319 Gateway Return to top The content for this page was generated ... -
Deisseroth INTRSECT Collection
TypeCollection...transgenic recombinase-expressing animal lines and retro-grade expressing viruses delivering additional recombinases... -
Caltech Systemic Capsids
TypeCollection... AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top... -
Penn Vector Core Partnership with Addgene
TypeCollection...Please contact [email protected] with any questions. Return to top... -
Lentiviral Vector Guide
TypeGuide... Versus Gamma-Retrovirus Lentiviruses and gamma-retroviruses fall under the Retroviridae family. The genome...Glossary Lentiviruses are a type of retrovirus and belong to the Retroviridae family. Infection with lentiviruses...variety of cell types than retroviruses. Both lentiviruses and gamma-retroviruses use the same packaging ...packaged by gamma-retroviral packaging systems, and vice versa. For more about gamma-retroviruses, see Addgene's..., leading to disease. Lentivirus A retrovirus from the Retroviridae family. Characterized by long incubation...BIV), and feline immunodeficiency virus (FIV). Retroviruses are partially characterized by their ability...of viruses in this family is made of RNA. This retroviral RNA is reverse transcribed into DNA before being... -
Adeno-associated virus (AAV) Guide
TypeGuide...variant able to display neuronal retrograde transport, AAVrg. Retrograde transport refers to the direction.... Browse Addgene’s in-stock viral preps . AAV retrograde (AAVrg) Directed evolution has also been employed...treat a diversity of diseases such as inherited retinal disease, spinal muscular atrophy, hemophilia A ...capable of both anterograde (from soma to axon) and retrograde (from axon to soma) transport. Many rAAV vectors... use with rabies virus (RABV) in monosynaptic retrograde tracing of neurons. In this system, the rAAV ...2016). A designer AAV variant permits efficient retrograde access to projection neurons . Neuron, 92 (2)... and the adenoviral genes provided in trans . Retrograde transport Movement of molecules, vesicles or ... -
Modular Cloning Guide
TypeGuide...) to optimize secretion and cell surface display of proteins of interest. Yeast Secrete and Detect Yeast...Penicillium or Aspergillus species. MoClo Yeast Secretion and Display (YSD) Toolkit Yeast Expression Paul...Plasmids to generate transcription units for protein secretion in yeasts S. cerevisiae and P. pastoris (a.k.a... MoClo-YTK toolkit for protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS...RBS strength variants, 8 tag-compatible RBSs, 2 secretion tags, 6 CDSs, 9 terminators, 4 nonfunctional "... -
CRISPR Guide
TypeGuide...coding sequence ~1 kb shorter than SpCas9 while retaining the same basic function. Its size allows it to...AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells that are poorly...Cas9 Fusion Tools dCas9 cannot cleave DNA, but it retains the ability to find specific DNA targets through...specificity, and editors carrying an ADAR truncation that retain RNA editing capabilities but are small enough to...23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...: 32572269 Charpentier, M., Khedher, A. H. Y., Menoret, S., Brion, A., Lamribet, K., Dardillac, E., Boix... -
Molecular Biology Reference
TypeGuide...plasmids through cell division. To ensure the retention of plasmid DNA in bacterial populations, an antibiotic...these conditions, there is a selective pressure to retain the plasmid DNA, despite the added replication ...to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect... -
Antibody Guide
TypeGuide... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects... your samples using permeabilization, antigen retrieval, and/or sectioning. Block to reduce non-specific...upwards of twenty. Laser and software capacity theoretically allow for roughly fifty different fluorophore... -
Cloning
TypeGuide...final product, with no undesired sequence ("scar") retained. A disadvantage of type IIS assembly cloning systems...restriction site. Because of the relatively long stretches of base pairing in the annealed product, ligation... -
Chemogenetics Guide
TypeGuide...chemogenetic access to optogenetic tools, they also retain the ability to be activated by standard optogenetics...chemogenetic control in a single molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005... -
Science Guides
TypeGuide...Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus Read More... -
Addgene's eBook Collection
TypeGuide...for choosing a fluorescent protein; information on FRET, biosensors, and monitoring protein localization...