We narrowed to 883 results for: prot

... Require Ex Vivo Handling of Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002... CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002...

...light can damage DNA and that proper personal protective equipment should be worn when visualizing using...for more detail. Find columnless purification protocols on our website. 6. Verify the plasmid After purifying...

...complexes to study neural function. Often the reporter proteins associated with these complexes are so robustly...vectors: packaging capacity and the role of rep proteins in vector purity." Human gene therapy 18.2 (2007...

...individual colonies growing out in the 96-well dish. My protocol with many additional tips can be found here. ...knockouts and knockins in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene...

...catecholamine neurons or glial fibrillary acidic protein (GFAP) for astrocytes it is important to test it...have low levels of TH mRNA without expressing TH protein indicating that there is promoter activity. Additionally...

... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo...

...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...binding to a specific configuration of a protein or a protein:protein complex. Due to their increased manufacturing...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...

... domain protein 1 2B28, UBXD10 ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2,...morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic protein 7 OP...TYROBP TYRO protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I...adaptor-related protein complex 3, beta 1 subunit ADTB3, ADTB3A, HPS, HPS2, PE AZGP1 alpha-2-glycoprotein 1, zinc-binding...shock 70kDa protein 1B FLJ54328, HSP70-1B, HSP70-2, HSPA1A HSPA1L heat shock 70kDa protein 1-like HSP70...HSPA2 heat shock 70kDa protein 2 HSP70-2, HSP70-3 HSPA4 heat shock 70kDa protein 4 APG-2, HS24/P52, MGC131852...

...Visualizing Protein–Protein Interactions Easy Split Fluorescent Proteins for Studying Protein–Protein Interactions...Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization...that can be used for protein purification, protein visualization, study of protein interactions, controlled...Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled Expression...using protease cleavage sites. Other protein tags and signal peptides are used to enhance protein solubility... out more! Protein Purification To study the function, structure, and activity of a protein it is generally...biofactories to express plasmids and produce proteins in high yields. Proteins can then be extracted from the culture...

...the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed by the serine protease TMPRSS2...encoding SARS-CoV-2 proteins, (spike protein, S; small envelope protein, E; nucleocapsid protein, N; and membrane...a cysteine endosomal protease that triggers proteolysis of SARS-CoV Spike protein, activating its membrane...SARS-CoV-2 nucleocapsid protein. Brian Geiss 211757 Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] Clone mBG86...membrane protein, M), including plasmids for protein production, secretion, and codon-optimized...TMPRSS2 plasmids - a serine protease that primes the SARS-CoV-2 S protein and is involved in virus entry...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential...

...binding protein Rac) RAF AFAF BRAF RAF1 Serine/threonine kinases: A-Raf proto-oncogene B-Raf proto-oncogene...GTPase and is a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular...known as MAPK; Mitogen-activated protein kinase ETS ETS1 ETS2 ETS proto-oncogene, transcription factor ...substrate JUN Jun proto-oncogene KSR KSR1 KSR2 Kinase suppressor of ras MDM2 MDM2 proto-oncogene MEK MAP2K1...known as MAP2K; Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase...phosphoinositide dependent protein kinase 1 PEBP1 Phosphatidylethanolamine binding protein 1 PIK3 Catalytic Subunits...RASA1 RASA2 RASA3 RAS p21 protein activator RASAL RASAL1 RASAL2 RASAL3 RAS protein activator like RASGRF ...

...fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein Common...Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further ...Another common scenario is fusing your protein to another protein, such as GFP, which allows you to...localization of your protein. Additionally, tags are commonly added to aid in protein purification. Just...production of His6-MBP-tagged fusion proteins in E. coli SUMO Protein purification pCIOX - Bacterial expression...tagged recombinant protein pDest-Sumo - For prokaryotic expression of target protein as N-terminal fusion...two sgRNAs from an hU6 and an mU6 promoter Protein-protein interaction Proximity-dependent biotin identification...

...ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ..., or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...edition) CRISPR software and depositor protocols Video protocol for genomic deletions in mammalian cell...applicability of base editing through protein engineering and protein delivery. Nature Communications . 8...

...truncated protein. Targeting an exon coding for an essential protein domain could abolish protein activity...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...modified mouse lines. Protein delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly...to translate mRNA to protein. Instead, you directly combine purified Cas protein and in vitro transcribed...Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and...our CRISPR Guide . There are many different Cas proteins available, and researchers can adapt these experiments...a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower...

...expression analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc...system is ideal for protein:protein interaction (PPI) studies, where each protein of interest is fused...useful for detecting and tracking key drug target proteins. Depositor...example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "...pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated gene editing...where you want minimal disruption of endogenous protein expression and interactions, such as exploring ...the virus of interest and monitoring endogenous protein dynamics in highly scalable assays. HaloTag Fusions...

... CRISPR Protein Tagging CRISPR Plasmids - Protein Tagging Browse CRISPR Plasmids...provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB Mendenhall...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...tagging techniques, as well as the plasmids and protocols needed for using them, are described below. Read...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and ...

...-producing proteins for detection of gene expression, protein localization, protein–protein interactions...Fluorescent Protein Guide for more plasmids containing fluorescent proteins. A set of fluorescent protein-based...plants. ID Plasmid Description PI Kits for Protein–Protein Interactions The following plasmid kits contain...variety of reporter plasmids for the study of protein–protein interactions in plants. ID Kit Description ...2in1 Plasmid Toolkit Plasmids for analysis of protein-protein interactions in planta via ratiometric Bimolecular...complementation (MoBiFC) toolkit for analyzing protein-protein interactions in plants. Golden Gate (MoClo)...expressing sgRNA and Cas9 in rice . Includes a protocol (Link opens in a new window) for targeted gene...

...For more details on protocols see the Addgene Protocols page or the Neuromab Protocols (Link opens in a ...then purified from the supernatant using Protein A or Protein G affinity columns. Formulation Buffer After...measured and the ratio of the antibody protein content to the total protein content of the sample is determined...secondary antibody. Protein expression is compared to an untransfected control and the protein size is confirmed...before distribution. Details about our production protocols, titering methods, and quality control are described...by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies, IPI will...these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage. Antibody...

... DNA repair protein, and is suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag...complementary protein function or localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ...ACP or MCP tagged plasmids for visualization of proteins inside cells. Depositor...Information New England Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ACP-, and... and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated with...features: Allows for visualization of multiple proteins simultaneously with dual labeling when used with...

...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...Engineering You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds...sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly after where the... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types...