... Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...express plasmid-based antibodies. ID Plasmid Description...

...production of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization... the genomic recognition site. NOTE: Figure 1 describes possible deletion strategies for genes and non-coding...deletion. For this approach, the internal primers described in step 2.1 may be omitted. CRISPR Cloning Anneal...plasmids by electroporation 4 . This protocol is described in detail for murine erythroleukemia (MEL) cells...editing efficiency, but 37 °C is acceptable. Fluorescence Activated Cell Sorting (FACS) of Transfected...

... Users may still generate their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

...3-fold repress on targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR as an...expression cassette, useful for selection or fluorescent gating of transiently transfected cells. Co-expression...can be ordered via the links below: ID Plasmid Description 44246 pdCas9-humanized A catalytically inactive...

...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more....Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...

...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains... Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning... and Eric Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the...

...and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI ...vector; for evaluation of shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA...transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast... M, Spangenberg C, Fees S, Fraser S, Eshkind L, Oesch F, Zabel B. Physiol Genomics . 2002 Dec 3;11(3):...

...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...inhibit the target neuron. For more detailed descriptions of chemogenetic receptors, ligands, and how ...preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags PI Do you...

...Service: Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism...Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various serotypes suitable... Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various serotypes suitable...

...strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination between... plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral For recombining... be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain...

...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

... COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection...Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...

...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...

...fused to a fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy...may also be fused to gRNA sequences to produce fluorescence at a target region, as in the CRISPRainbow kit...

...option. Coupling brighter bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive... routes. Image courtesy of Ute Hochgeschwender . Table 3. Descriptions of LMOs and their activity in neurons... alternative DREADD ligands. Compound 21, Deschloroclozapine (DCZ), Perlapine, and Olanzapine have all...bidirectional control of neuronal activity. Table 1. Descriptions of DREADDs and their activity in neurons DREADD...their effect, and outcome in neurons. Table 2. Descriptions of PSAMs and their activity in neurons PSAM ... PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic fusion proteins...Barter JW, Rossi MA, Augustine GJ, Yin HH, Hochgeschwender U (2016). Luminopsins integrate opto- and chemogenetics...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...Mammalian U6x2 yes, nick S. pyogenes Puro Jackson pBluescriptSKII+ U6-sgRNA(F+E) empty 74707 Mammalian hU6 none...from our collection. Plasmids Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit ...simultaneously repressing multiple target genes in Escherichia coli. Koffas Do you have suggestions for other...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...performs a chemiluminescent reaction in the presence of several different substrates. The luminescence produced...wells) by fluorescence activated cell sorting (FACS). Figure 7: Flow cytometry uses fluorescently-labeled...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent... to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...

...27. See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...For more information, see the bioRxiv preprint describing ICE . Deep Sequencing Data Analysis CRISPResso... featured on Addgene's blog Protocols Lab(s) Description Plasmids in protocol Download protocol Church...

...recognizes the different fluorescent labels. The machine detects which fluorescently labeled nucleotide is...some common plasmid elements: Plasmid Element Description Origin of Replication ( ori ) DNA sequence which...the control of a non-bacterial promoter) or a fluorescent protein (that can be used to select or sort the...commercial lab strains of E. coli used today are descended from two individual isolates, the K-12 strain ...relies on the random incorporation of modified, fluorescently tagged bases onto the growing DNA strand in ...process occurs when the polymerase incorporates a fluorescently tagged nucleotide. Because these special bases...nucleotide, the reaction is halted once the fluorescently tagged base is incorporated. Sanger sequencing...