Using AAV for Neuronal Tracing
Type
Blog Post
...transport is typically used for the trafficking of organelles, such as mitochondria, as well as macromolecules...
We narrowed to 614 results for: GAN
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Technical Design of a Western Blot
TypeBlog Post...blog Antibodies 101: The Basics of Western BlottingAntibodies 101: Selecting the Right AntibodyAntibodies... -
Adeno-associated virus (AAV) Guide
TypeGuide...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...different serotype(s) for transduction of a given organ. Tissue Serotype Central nervous system AAV1, AAV2...administration, distributing widely throughout the organism to target large or diffused biological structures... of systemic rAAV vectors that can target whole organs or specific cell types of the CNS (e.g. AAV-PHP.eB... transport Movement of molecules, vesicles or organelles from the main cell body, or soma, towards the...pathogen, like viruses or bacteria, within a host organism, leading to disease. Insertional mutagenesis Random... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...import of newly synthesized (green) protein and organelles with older (yellow to orange) protein. Image ... -
Modular Cloning Guide
TypeGuide...at 18 levels for rapid characterization in new organisms. Mobius Assembly Vector Toolkit Bacterial Expression...reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial...Platinum TALENs for use in a wide variety of target organisms. TALEN Kit Genome Engineering, TALEN Stephen Ekker...fusion proteins for use in many different model organisms. Diatom uLoop Assembly Kit Other, Yeast Expression...tags, and signal peptides, for use in a range of organisms including diatoms, yeast, plants and bacteria.... -
CRISPR Guide
TypeGuide...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG...Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of..., E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., Ter-Ovanesyan, D., Braff, J. L., Davidsohn...23907171 Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,... -
Optogenetics Guide
TypeGuide...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...LAD Light-activated dimerization, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain...engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain... -
Molecular Biology Reference
TypeGuide... A, T, C, and G, respectively). The bases are organized into a right-handed, double-stranded helix. To...the instructions to make every protein within an organism. Before proteins can be translated, the DNA is...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and cultured human... -
Gamma-Retroviral Vector Guide
TypeGuide...Hussain, R., Banani, M. A., Day, W., Stevenson, E., Madigan, M., Chen, J., Khinder, R., . . . Pule, M. (2022...pathogen, like viruses or bacteria, within a host organism, leading to disease. Provirus The genetic material... -
Plan Your Experiment
TypeGuide...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have... -
Lentiviral Vector Guide
TypeGuide... Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996...pathogen, like viruses or bacteria, within a host organism, leading to disease. Lentivirus A retrovirus from... -
Antibody Guide
TypeGuide...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This... -
Promoters
TypeGuide...promoter region controls when and where in the organism your gene of interest is expressed. Promoters ... -
Adenovirus Guide
TypeGuide...pathogen, like viruses or bacteria, within a host organism, leading to disease. Innate immunity Non-specific...