...close enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic..., a large portion of the Cas9-induced DSBs will still be repaired via NHEJ. The resulting population of... be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting requires delivery...CAPTURE system, developed by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor...

... simpler than eukaryotic transcription bacteria still have complex systems of gene regulation, like operons...opposite way. The operon is normally transcribed until a repressor/corepressor binds to the operator preventing...

...technologies developed for optogenetics research utilize light to detect, measure, and control molecular...

...using helper-free packaging cell lines This method utilizes a packaging cell line that stably expresses Gag-Pol...

... sequencing primer" and "3' sequencing primer". Still not sure what primer you need? Email us at help@...

...desired edit, as a large percentage of DSBs are still repaired by NHEJ. How do you determine that your...

...bind to a protein is powerful, but in order to utilize it in the lab, researchers must be able to detect...