Transferable Skills Guide: Teamwork
Type
Blog Post
...have - sometimes you just don’t know who you need until the team really starts functioning (or malfunctioning...
We narrowed to 617 results for: TIL;
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Antibodies 101: Flow Cytometry
TypeBlog Post...different target. Flow cytometers however are highly versatile and some can accommodate up to 30 different colors... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post... overview and comparison of three of the most versatile biosensor biorecognition elements. Biosensors ... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...paper at Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for rapid combinatorial ... -
Molecular Cloning Techniques
TypeGuide...in the DNA. In restriction cloning, scientists utilize specific restriction enzymes to cut dsDNA of interest...reducing the amount of time required for cloning. To utilize this approach, the fragment of DNA that you would...pairs adenine (A) and thymine (T). TOPO cloning utilizes the Taq polymerase, which naturally leaves a single...traditional restriction enzyme cloning. Scientists have utilized this approach to create compatible custom overhangs...polymerase will continue to function as an exonuclease until a base is exposed on the single-strand overhang ...100 kb) compared to other cloning methods that utilize bacteria, where it becomes progressively more difficult...which pieces of DNA that share no homology ends can still be fused in a seamless manner. To accomplish this... -
Optogenetics Guide
TypeGuide...for light-induced cAMP modulation. Utilizes BLUF (blue light utilizing FAD) domains. 441 Phytochromes BphP1...technologies developed for optogenetics research utilize light to detect, measure, and control molecular...domains from two different ChRs. These ChR variants still function as light-gated, cation channels resulting...used, there is an incubation time (days to weeks) until peak expression of the opsin is reached. This approach...figure above, this light induced binding can be utilized to control the localization of a protein of interest... -
Chemogenetics Guide
TypeGuide...cross the blood-brain barrier in rats, and the utility of DREADDs in experiments in this species may be... as laser or LED illumination), making LMOs a versatile option. Coupling brighter bioluminescent modules...reporter ensures that the reporter remains silent until a cell or tissue-specific Cre is provided. Download...genetically-encoded bioluminescent opsins for versatile, scalable, and hardware-independent optogenetic... -
Plan Your Experiment
TypeGuide...cell type. Figure 2: Different delivery systems utilized for CRISPR/Cas gene editing. This image summarizes...contains a selection marker. Plasmid delivery is versatile thanks to the wide range of options for the Cas...also transient, as the RNPs will only be active until they are degraded. This method is helpful in cells...will ultimately depend on which CRISPR method you utilized. If your edit affects genome sequence, your edited... -
Molecular Biology Reference
TypeGuide... DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert or other ...primers page . Types of Plasmids Plasmids are versatile and can be used in many different ways by scientists...Sanger sequencing (Figure 4). Like Sanger, NGS utilizes modified, fluorescently-tagged nucleotides. During... -
CRISPR Guide
TypeGuide...close enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic..., a large portion of the Cas9-induced DSBs will still be repaired via NHEJ. The resulting population of... be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting requires delivery...CAPTURE system, developed by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor... -
Lentiviral Vector Guide
TypeGuide...replicate on their own. While the viral components are still separated on different plasmids, the viral genome...followed by transduction is necessary to properly utilize lentiviral vectors. Some lentiviral plasmids (mostly...knowing the general preference for integration, it is still random and difficult to predict. In therapeutic ... -
Promoters
TypeGuide...simpler than eukaryotic transcription, bacteria still have complex systems of gene regulation, like operons...opposite way. The operon is normally transcribed until a repressor/corepressor binds to the operator preventing... -
Science Guides
TypeGuide...technologies developed for optogenetics research utilize light to detect, measure, and control molecular... -
Sequencing Primers
TypeGuide...Addgene’s protocol to design your own primers . Still have questions about choosing the best primer for... -
Adenovirus Guide
TypeGuide...creating first-generation rAdV vectors, which is still the most commonly used generation for research purposes... -
Adeno-associated virus (AAV) Guide
TypeGuide...delivery of larger transgenes, but expression is still much less efficient than with a single rAAV vector... -
Antibody Guide
TypeGuide...bind to a protein is powerful, but in order to utilize it in the lab, researchers must be able to detect...