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Showing: 601 - 620 of 707 results

  1. Plasmid Collections

    Type
    Collection
    ...Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics...and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more.... set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways ...collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology...

  2. Optogenetics Plasmid Collection

    Type
    Collection
    ...Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical...neuronal activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used ...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the... to tag endogenous loci with epitope tags or fluorescent markers. Future Directions It’s amazing to see...for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product....

  4. Microbiology Resources

    Type
    Collection
    ...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning...

  5. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Science has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in... Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids... collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell...plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...

  6. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

  7. Institute for Protein Innovation

    Type
    Collection
    ... Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...express plasmid-based antibodies. ID Plasmid Description...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...production of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization... the genomic recognition site. NOTE: Figure 1 describes possible deletion strategies for genes and non-coding...deletion. For this approach, the internal primers described in step 2.1 may be omitted. CRISPR Cloning Anneal...plasmids by electroporation 4 . This protocol is described in detail for murine erythroleukemia (MEL) cells...editing efficiency, but 37 °C is acceptable. Fluorescence Activated Cell Sorting (FACS) of Transfected...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... Users may still generate their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

  10. Tetracycline Inducible Expression

    Type
    Collection
    ...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...expression of your gene of interest. ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro... shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target...transactivator for your tetracycline system. ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast... for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression...

  11. Distribution to Industry

    Type
    Collection
    ... COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection...Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards... multigene constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for ...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...

  12. Control AAV Preps

    Type
    Collection
    ...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more....Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...

  13. TALEN Plasmids and Kits

    Type
    Collection
    ...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains... Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning... and Eric Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the...

  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...mammalian expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6...pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3...pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression ... with multiple cloning sites Browse our full Fluorescent Protein (FP) Guide and the Michael...

  15. Chemogenetics Plasmids

    Type
    Collection
    ...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...inhibit the target neuron. For more detailed descriptions of chemogenetic receptors, ligands, and how ...preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags PI Do you...

  16. Serotype Testing AAV

    Type
    Collection
    ...Service: Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism...Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various serotypes suitable... Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various serotypes suitable...

  17. Adenovirus Plasmids

    Type
    Collection
    ...strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination between... plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral For recombining... be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain...

  18. Antibody Production

    Type
    Collection
    ...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

  19. Promega Plasmid Collection

    Type
    Collection
    ..."HaloTag", "BiT", or other terms. ID Plasmid Description Industry Plasmid Technology Overview This collection... detection. It can be paired with LgBiT for bioluminescent assays or with the high-affinity anti-HiBiT... form an active enzyme and generate a bright luminescent signal in the presence of a substrate. NanoBiT...

  20. TALEN Guide

    Type
    Collection
    ...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...
Showing: 601 - 620 of 707 results