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...depositor’s protocol for the best results. If you have any questions about the library protocols, feel free... Library Amplification protocol. See our Pooled Library Amplification Protocol for Addgene's generalized...generalized library amplification protocol. Read our CRISPR guide for more information on genome-wide screening...

...PSAM-5HT3 LGICs pair a PSAM domain with a 5-HT 3 serotonin receptor sodium- or potassium-selective IPD. Binding...molecule driven by FRET-modified bioluminescence. Neurophotonics 11 (2), 021005. https://doi.org/10.1117/1.NPh...., Xiong, Y., Urushihata, T., Hirabayashi, T., Fujimoto, A., Mimura, K., English, J. G., Liu, J., Inoue...Inoue, K. I., Kumata, K., Seki, C., … Minamimoto, T. (2020). Deschloroclozapine, a potent and selective ...luminopsins with improved coupling efficiencies. Neurophotonics, 11 (2), 024208. https://doi.org/10.1117/1....

...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...10.1038/s41587-020-0490-7 PMID: 29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the...editing experiments in mammalian cells. Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/...

...collection of 93 modular plasmids and enhanced MoClo protocols for rapid one-pot, multipart assembly, combinatorial...Engineering, CRISPR, Mammalian Expression Takashi Yamamoto Plasmids for construction of all-in-one CRISPR...Accessory Pack Genome Engineering, TALEN Takashi Yamamoto Nine pFUS array vectors and destination vectors...Gate TALEN Kit Genome Engineering, TALEN Takashi Yamamoto Plasmids for construction of highly-active Platinum...

... models. For more detailed protocols, see Addgene’s Viral Vector Protocols . Figure 3: Overview of rAAV...Liver AAV8, AAV9 Lung AAV6 Pancreas AAV2, AAV8 Photoreceptor cells AAV2, AAV5, AAV8 Skeletal muscle AAV1,...Vectors 101 topics More AAV Vector topics Addgene Protocols General Transfection AAV Production in HEK293T...

...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification Methods...binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication. This...Special considerations Many of the steps in this protocol vary depending on assay and microscopy techniques... may be a fluorophore or an enzyme. The basic protocols and controls follow that of IF (above). Sample...

..., K. W., Vogelstein, B., & He, T. C. (2007). A protocol for rapid generation of recombinant adenoviruses...adenoviruses using the AdEasy system . Nature Protocols, 2 (5), 1236–1247. https://doi.org/10.1038/nprot.2007.135...17546019 (Link opens in a new window) Matsunaga, W., & Gotoh, A. (2023). Adenovirus as a vector and oncolytic...

...bacterial cultures with antibiotics, see Addgene’s protocol on inoculating bacterial cultures . Antibiotic...specialized membranes containing nanopores (Figure 5). A motor protein then unwinds double-stranded DNA, and a ...Plasmids 101 series on the Addgene blog Addgene Protocols Content last reviewed: 22 October 2025...

... annotated primers. You can also use Addgene’s protocol to design your own primers . Still have questions...

...Plasmid Backbone Genome Eengineering Guide Addgene Protocols Plasmid cloning by restriction digest Plasmid ...

... Protocols Pipetting Pipetting Protocol Intro to the Lab Bench Check out more protocols and videos...negatively impact your experimental results. This protocol will guide you in selecting the correct pipette...Information Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger at the top,... pipette tip off of the pipette. This side view photo of a P1000 pipette shows the volume adjustment ring...different mechanisms to disperse liquids, this protocol is focused on single channel pipettes. These come...ready to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving ...a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn more....

...transformations. Last Update: Nov. 13, 2017 Protocol Video Watch the protocol video below to learn how to isolate... Protocols Bacterial Transformation Bacterial Transformation...higher efficiency is needed follow the complete protocol. Thaw the competent cells in your hand instead...

... Protocols Gibson Cloning Gibson Assembly Cloning You may also...Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson and colleagues...

...Weighing Reagents Protocol Intro to the Lab Bench Check out more protocols and videos to help you get started...In this protocol you will learn the basics of how to weigh reagents. Protocols... Protocols Weighing Reagents Weighing...affect the outcome of your experiment. In this protocol, you will learn the basics of how to weigh reagents...will be weighed out Procedure The balance in the photo above has a capacity of 200 g, as indicated by the... Make sure that any spills produced during the protocol are cleaned up. Discard the weighing boat or weighing...

... Protocols Water Bath Water Bath Protocol Intro to the Lab Bench Check out more protocols and videos...between 4°C and room temperature. This general protocol suits almost any water bath. Video Watch the video...the appropriate temperature as described in your protocol. How to set the temperature will vary depending...

...plate. Last Update: Feb. 28, 2017 Protocol Video Watch the protocol video below to learn how to isolate... Protocols Streaking Bacteria on LB Agar Plate Streaking ...mixture of plasmids in your purified DNA. This protocol explains how to isolate a single bacterial colony...

...Protocols for plasmid cloning by PCR.... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may...

...-restriction site-your sequence). Protocol Video Watch the protocol video below to learn how to design... Protocols Designing Primers How to Design a Primer You may also like...

...In this protocol you will learn the basics of how to use a light microscope.... Protocols Using a Light ...Microscope Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab!...microscopy and still have more to learn, so, for this protocol, we'll focus on one of the most common types that...

...website . Last Upload: May 18, 2018 Protocol Video Watch the protocol video below to learn how to analyze...Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and... Protocols Diagnostic Restriction Digest Diagnostic Restriction Digest You may also like... Restriction...should have the correct orientation. For detailed protocols as well as tips and tricks see the Restriction...