...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification Methods...binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication. This...Special considerations Many of the steps in this protocol vary depending on assay and microscopy techniques... may be a fluorophore or an enzyme. The basic protocols and controls follow that of IF (above). Sample...

...Library Amplification Protocol for Addgene's generalized library amplification protocol. Read our CRISPR guide...amplify the library, please refer to the depositor’s protocol for the best results. For some libraries, plasmid...

...McElligott ZA, Holmes A, Heisler LK, Kash TL. 2016. Serotonin engages an anxiety and fear-promoting circuit ...

...such as adenovirus or herpes simplex virus, or a genotoxic agent such as UV radiation or hydroxyurea, is ... AAV4, AAV5, AAV6, AAV9 Pancreas AAV6, AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment ...

... Protocols Pipetting Pipetting Protocol Intro to the Lab Bench Check out more protocols and videos...negatively impact your experimental results. This protocol will guide you in selecting the correct pipette...Information Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger at the top,... pipette tip off of the pipette. This side view photo of a P1000 pipette shows the volume adjustment ring...different mechanisms to disperse liquids, this protocol is focused on single channel pipettes. These come...ready to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving ...a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn more....

...transformations. Last Update: Nov. 13, 2017 Protocol Video Watch the protocol video below to learn how to isolate... Protocols Bacterial Transformation Bacterial Transformation...higher efficiency is needed follow the complete protocol. Thaw the competent cells in your hand instead...

... Protocols Gibson Cloning Gibson Assembly Cloning You may also...Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson and colleagues...

...Weighing Reagents Protocol Intro to the Lab Bench Check out more protocols and videos to help you get started...In this protocol you will learn the basics of how to weigh reagents. Protocols... Protocols Weighing Reagents Weighing...affect the outcome of your experiment. In this protocol, you will learn the basics of how to weigh reagents...will be weighed out Procedure The balance in the photo above has a capacity of 200 g, as indicated by the... Make sure that any spills produced during the protocol are cleaned up. Discard the weighing boat or weighing...

... Protocols Water Bath Water Bath Protocol Intro to the Lab Bench Check out more protocols and videos...between 4°C and room temperature. This general protocol suits almost any water bath. Video Watch the video...the appropriate temperature as described in your protocol. How to set the temperature will vary depending...

...plate. Last Update: Feb. 28, 2017 Protocol Video Watch the protocol video below to learn how to isolate... Protocols Streaking Bacteria on LB Agar Plate Streaking ...mixture of plasmids in your purified DNA. This protocol explains how to isolate a single bacterial colony...

...Protocols for plasmid cloning by PCR.... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may...

...-restriction site-your sequence). Protocol Video Watch the protocol video below to learn how to design... Protocols Designing Primers How to Design a Primer You may also like...

...In this protocol you will learn the basics of how to use a light microscope.... Protocols Using a Light ...Microscope Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab!...microscopy and still have more to learn, so, for this protocol, we'll focus on one of the most common types that...

...website . Last Upload: May 18, 2018 Protocol Video Watch the protocol video below to learn how to analyze...Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and... Protocols Diagnostic Restriction Digest Diagnostic Restriction Digest You may also like... Restriction...should have the correct orientation. For detailed protocols as well as tips and tricks see the Restriction...

...lengths). Last Update: Feb. 20, 2018 Protocol Video Watch the protocol video below to learn how to perform...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and... Protocols Agarose Gel Electrophoresis Agarose Gel Electrophoresis You may also...

...Protocol - How to Ligate Plasmid DNA... Protocols DNA Ligation DNA Ligation You may also like... Plasmid... to fuse the two ends of the vector together). Protocol: Standard Insert + Vector DNA Ligation Before ...

...Watch the protocol video below to learn how to inoculate bacteria in liquid culture. Protocol Prepare liquid...Protocol for Inoculating a Bacterial Culture Protocols... Protocols Inoculating a Liquid Bacterial Culture Inoculating...plasmid DNA for experimental use. The following protocol is for inoculating an overnight culture of liquid...cloudy haze in the media (see right). Notes: Some protocols require bacteria to be in the log phase of growth...growth. Check the instructions for your specific protocol and conduct an OD600 to measure the density of...

... Protocols pLKO.1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878. Protocol Version ... RNAi Tools page. E.2 Protocol for Producing Lentiviral Particles This protocol is for transfection in...Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting...Copyright Addgene 2006, All Rights Reserved. This protocol is provided for your convenience. See “warranty...Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles F. Infecting...Determining the optimal puromycin concentration F.3 Protocol for lentiviral infection and selection G. Safety...competent DH5 alpha cells, following manufacturer’s protocol. Plate on LB agar plates containing 100 μg/mL ...

... Protocols Sequence Analysis Sequence Analysis of a Plasmid...

...concentration. Last Update: Oct. 27, 2017 Protocol Video Watch the protocol video below to learn how to spread... Protocols Streaking Bacteria on LB Agar Plate Over-Agar ...Plasmid DNA from Bacterial Culture Introduction This protocol describes methodology for plating antibiotic over-agar...separate batches of antibiotic-containing agar. This protocol will focus specifically on selection with carbenicillin...transformation page for a detailed heatshock transformation protocol. Plate 50 µL of transformed E. coli /rescue media...