We narrowed to 702 results for: BASE;

...chromatin. Base Edit : CRISPR base edit libraries use gRNAs to guide base editors to convert one base to another...target genes for creating insertions, deletions, and base edits. Other : CRISPR libraries have also been designed...library. The process diverges for lentiviral or DNA-based screens. Created with BioRender.com. Types of Pooled...

...Multi-Color Imaging FPbase (Link opens in a new window) : an open-source, community-editable database for fluorescent...fluorescent protein. Data on this page are compiled from FPbase (Link opens in a new window) or plasmids' original...

...of components (Weber et al., 2011). This method, based on Golden Gate plasmid assembly, exploits the ability...Ellis Plasmids for constructing highly-tunable GPCR-based biosensors in yeast (requires MoClo-YTK parts for...O'Callaghan Toolkit for assembling large DNA fragments based on methylation-switching. 4G-Cloning Plasmid Kit...assembly of cellular circuits using Golden Gate-based cloning methods for use in mammalian cells. These...Plasmids to assemble custom-made RNA-binding proteins based on the Pumilio and FBF (PUF) homology domain, for...Nekrasov 95 plasmids including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for genome...

...Resources FPbase (Link opens in a new window) : an open-source, community-editable database for fluorescent...

...increase flexibility or specificity in antibody-based methods. Antibodies can recognize and bind other...produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary .... The complexity of this can vary significantly based on the antibody and conjugate chosen. Signal Amplification...assay. Overview of Antibody Applications Antibody-based applications can be generally classed into four ...Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify exact...strategy. Figure 5: Immunoprecipitation using bead-based antibody capture to isolate specific proteins from... cells are then slotted in a specific direction based on the emission(s) read by the machine. Controls...

...Phytochrome, cryptochrome, and LOV (light oxygen voltage)-based systems have been used in many experimental contexts...Blue-light–mediated induction of protein interactions based on Arabidopsis thaliana cryptochrome 2 and CIB1 ...to control calcium channels ~488 LOV domains LOV-based Light-oxygen-voltage-sensing (LOV) domains are protein...LINUS light-inducible nuclear localization signal based on the LOV2 domain of Avena sativa phototropin 1...blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible Dronpa mutant domains that associate...References and Further Reading OptoBase (Link opens in a new window) annotated database of optical switches and...

...Plasmids 101 September 2023 | Fourth Edition About Based on our popular Plasmids 101 blog series, this eBook...practical introductions and helpful tips for antibody-based applications, from flow to IHC! Close About Overlay...

...produce AAV vectors. Additional production systems based on other human cell lines, such as HeLa and A549...low risk of insertional mutagenesis. Several AAV-based gene therapies have been approved by the US Food...muscular dystrophy. Many clinical trials of AAV-based gene therapies are underway and are promising for...one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...the rAAV capsid, such as Cas9-derived plasmids and base/prime editors, is to create split AAVs. Here, a ...genes provide a helper function for transfection-based adeno-associated virus vector production . Journal...

...genetic cargo into cells. Most rAdV vectors are based on adenovirus type 5 (Ad5) and have been produced... adenoviral vector production system. Figure is based on the use of pAdEasy-1. AdMax™ AdMax™ is another...be very useful for designing and producing rAdV-based vaccines. Adenoviral vectors are excellent vectors...delivery: potential applications for gene and cell-based therapies in the new era of personalized medicine...

...interest is expressed. Promoters are about 100–1,000 base pairs long and are adjacent and typically upstream...The proximal promoter is found approximately 250 base pairs upstream from the TSS and is the site where...regulatory elements, such as enhancers, several kilobases away from the transcription start site (TSS). ...

... National Cancer Institute (NCI) NCI Biomarker Database International Cancer Genome Consortium...

...www.thesgc.org/scientists/groups/toronto Protein Database (PDB): www.pdb.org Request Plasmids To order, ...

...blog post . Data on this page are compiled from FPbase (Link opens in a new window) or plasmids' original...

...degradation. This degradation can continue for many kilobases in one or both directions from the target region...

...plasmid with your gene of interest? Search our database for shuttle plasmids with inserts. ID Plasmid ...

... Check out Addgene’s Help Center, a searchable database of information to help you every step of the way...

... ligands. Data is embedded in an integrated knowledgebase that features, among others, genetic variants...

...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...

...highly specific binding to other proteins. Antibody-based applications, which often use antibodies conjugated...

...including knockout, inhibition/activation, and prime and base editing. Browse Addgene's collection of CRISPR libraries...virus. The potential for oncogenesis is largely based on whether or not the specific insert contained ... Swaminathan, T. A., & Braun, S. E. (2021). HIV-based lentiviral vectors: Origin and sequence differences...