...chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila... recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced. Cre-mediated...repair templates (ie. PCR-derived dsDNA) with 30-60 basepair homology arms to knock in large epitopes, such...

.... Eggeling C, Willig KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics...

...PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo spectral analysis of fluorescent proteins...

...using the virus. You may need to adjust coordinates based on your own method of identifying bregma but it ...

...mutation or inserting a tag Cas9 (or Cas9 nickase); Base editor Single (or dual) gRNA targeting the region...displays reduced efficiency compared to NHEJ knockout. Base editors can make a limited set of mutations. Repress...and select a target sequence nearby. Select gRNAs based on predicted on-target and off-target activity A... target sequences and rank the associated gRNAs based on their predicted on-target and off-target activity...experimental system. CRISPR efficiency will vary based on the method of delivery and the cell type. Before...into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. ... delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nature Biotechnology...

...types, and most adenoviral vectors are based on Ad5. Ad5-based vectors use the Coxsackie-Adenovirus Receptor...elements that promote bi-directional transcription Ad5-based vector Most common type of adenoviral vector pAdTrack...-Adenovirus Receptor (CAR) Receptor used by Ad5-based vectors to enter cells Return to Top...

...increase flexibility or specificity in antibody-based methods. Antibodies can recognize and bind other...produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary .... The complexity of this can vary significantly based on the antibody and conjugate chosen. Signal Amplification...assay. Overview of Antibody Applications Antibody-based applications can be generally classed into four ...Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify exact...strategy. Figure 5: Immunoprecipitation using bead-based antibody capture to isolate specific proteins from... cells are then slotted in a specific direction based on the emission(s) read by the machine. Controls...

...activated. Optogenetics tools can be broadly classified based on their functions into two groups: Actuators are...Phytochrome, cryptochrome, and LOV (light oxygen voltage)-based systems have been used in many experimental contexts...Blue-light–mediated induction of protein interactions based on Arabidopsis thaliana cryptochrome 2 and CIB1 ...to control calcium channels ~488 LOV domains LOV-based Light-oxygen-voltage-sensing (LOV) domains are protein...LINUS light-inducible nuclear localization signal based on the LOV2 domain of Avena sativa phototropin 1...blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible Dronpa mutant domains that associate...profile 282 References Optogenetic Switches from OptoBase, W. Weber Lab Edward Boyden's Lab: Synthetic Neurobiology...

...Plasmids 101 September 2023 | Fourth Edition About Based on our popular Plasmids 101 blog series, this eBook...practical introductions and helpful tips for antibody-based applications, from flow to IHC! Close About Overlay...

... to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions...

...transduction. Many lentiviral transfer plasmids are based on the HIV-1 virus. For safety reasons, transfer...included in transfer plasmids include: Tet- or Cre-based regulation and fluorescent fusions or reporters....Functional restriction sites must be a certain number of bases from the ends of the primers used. Alternatively... cell. The potential for oncogenesis is largely based on the specific insert contained within the lentiviral...

... expressed. Summary Promoters are about 100-1000 base pairs long and are adjacent and typically upstream...The proximal promoter is found approximately 250 base pairs upstream from the TSS and it is the site where...regulatory elements such as enhancers several kilobases away from the TSS. Eukaryotic promoters are so...

...Cap, flanked by two 145 base inverted terminal repeats (ITRs). These ITRs base pair to allow for synthesis...

...highly specific binding to other proteins. Antibody-based applications, which often use antibodies conjugated...

...plasmids in our repository, we've chosen primers based on the plasmid backbone and insert. To identify ...

...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...

...Immunology Research Genes have been grouped into tables based on the Gene Lists from Immport . Click the links...grouped in to the CC, CXC, XC, and CX3C families based on conserved cysteine residues. Chemokines recruit...

... , Nature Methods 2016 Jul;13(7):557-62. FPbase : A database for users of fluorescent proteins created... Nikon Imaging Center at UCSF / QB3 Wiki FPbase : A database created by Talley Lambert at Harvard Medical...Scholarpedia : Entry by Robert Campbell Spectra Database : Graphing site hosted at the University of Arizona...

... CasPEDIA is meant to be a constantly updating database which serves as a resource for the scientific ...properties (Makarova et al). CasPEDIA is an evolving database, and all researchers are invited to contribute..., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: A Functional Classification System for Class ...

...exhaustive, so you may want to consult the REBASE database for more detailed information. Enzyme Dam...regulating genome replication, repairing mismatched basepairs or small indels that occur during DNA synthesis...