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We narrowed to 1,146 results for: Gene

Showing: 661 - 680 of 1146 results

  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...recombinant adeno-associated viral vectors." Human Gene Therapy ja (2019). PubMed PMID: 31140327. PubMed...vector genome titers by droplet digital PCR." Human gene therapy methods 25.2 (2013): 115-125. PubMed PMID...found that generating the standard curve for qPCR can be challenging to get right. Here at Addgene, we use...droplet quality. Using a droplet generator, the sample and the droplet generation oil are moved through small...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ...virus before adding your sample to a mastermix. At Addgene, we typically load 3 dilutions onto our ddPCR plate...Pipette slowly If you are using a manual droplet generator, you will have to be careful about transferring...

  2. Tips for arabidopsis transformation

    Type
    Blog Post
    ...transcriptional and translational dynamics of genes and proteins in living plants, to complementing mutant phenotypes...potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop....simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org Find plant... transformation. There are many motivations to generate transgenic Arabidopsis, from studying transcriptional... floral drip or dip transformation method. The general steps for this method include: Cloning and transforming...Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing an expression system Choosing an appropriate...appropriate expression vector is the first step in generating transgenic Arabidopsis. There are a bunch of ...

  3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using CRISPR ...tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...quantitative imaging experiments and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing...cellular compartments other than the cytoplasm. Generally, FPs evolved in or were engineered for use in ...N-glycosylation consensus sequences in popular FPs. This generated a palette of inert green, cyan, yellow, and blue...standard FPs. The moxFPs are now available through Addgene Many thanks to our guest bloggers Erik L. Snapp...Central PMCID: PMC2150754. Resources Avaliable at Addgene Read Our Blog Post on Protein Tags Read Other ...

  4. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great Tools for Gene Expression...Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from Addgene ...Expression in Mammals Resources on Addgene.org Find AAV Packaging and Transfer Plasmids Here New to Making...

  5. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...promoter. Use this for doxycycline inducible (tet-off) gene expression! We've also added AAV1 and AAV5 preparations...variants of a new optogenetic tool called PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...to our viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser...AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...

  6. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...Gladys will use plasmids containing a variety of HPV genes as positive controls in her PCR screens. She hopes... Sciences in Ghana will be receiving the third Addgene/Seeding Labs plasmid grant and plans to use the...future. Future awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of ...inspiring research. Additional Resources on the Addgene Blog Learn How We Raised Money for Rare Disease...Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer Pages Learn about Global...

  7. Editor's Choice, July 2016

    Type
    Blog Post
    ...the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression in Mammals Recombinase-based State Machines...excellent posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR... them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the...perturbations to how CRISPR has made it much easier to generate mouse models.   Cpf1 Update: Comparison to Cas9... Science Communications Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on ...

  8. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...6 letter representation of the gene (or DNA sequence). pBACKBONE-hGene Tip: Often researchers will add...pBACKBONE-Flag-hGene(Q295A)-EGFP  Wildtype version: pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt)-EGFP...insert, you would list it first. pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal... would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains...be included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...fluorescent biosensors to measure biomolecules or genes via FRET or other assays....specific biomolecules, the activity of specific genes and cellular processes, or other factors like environmental...mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial content, structure, stress and...for a sensor for a different analyte, a sensor for gene-specific activity, or for popular biosensors in ...mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible for Viral Packaging ...also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids ...Gadella Calcium Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca(2+) indicator...

  10. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ...Prof Jim Collins and specialising in engineering gene regulation in yeast. Dr. Ellis is now one of the...Today’s episode of the Addgene Podcast features guest host and Addgene European Outreach Scientist, Benoit...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...it was a short journey for Benoit to interview Addgene Advisory Board Member and lead researcher at Imperial...Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest, Tom Ellis! Dr. Ellis...direct interactions between drugs and promoters of oncogenes. He returned to academic research in 2005 to investigate...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...

  11. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ... Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Senaratne is a graduate of the PhD program in Genetics and Genomics at Harvard Medical School, and is...is currently a fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

  12. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ... Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Senaratne is a graduate of the PhD program in Genetics and Genomics at Harvard Medical School, and is...is currently a fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

  13. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ...Use cloning grade DNA for direct cloning of your gene of interest into a plasmid backbone. Transfection...quantity and purity.  Additional resource on the Addgene blog Browse blog posts on plasmid cloning Read... conjugation, and transfection Resources on Addgene.org Read the molecular biology reference Find plasmids...

  14. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...protected. Effects of Activation of the PGC-1a1 Pathway Gene expression analysis of the animals’ skeletal muscles...protects the brain from depression. Mice that are genetically modified to produce extra PGC-1a1 only in their...psychiatric and cognitive conditions as well as neurodegenerative diseases.” I don’t know about you, but I think...right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC-1α1 modulates...

  15. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can skip this... perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do...Preparation of electrocompetent cells (protocol) At Addgene, we use the Mix & Go! E. coli Transformation Kit...large number of transformations being performed at Addgene, this adds up to a significant time savings. You...

  16. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...applications of fluorescent proteins, genetically encoded biosensors, and optogenetic tools. Follow her recent research...-the-art probes, many of which you can find at Addgene. Biliverdin chromophore Near-infrared fluorescent...FPs can be combined with blue-light controlled optogenetics, such as channelrhodopsins and tools based on...reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of a probe leads to less interference... Ref BphP-based FPs miRFP670 https://www.addgene.org/79987/ 642 670 87,400 14.0 198 Monomer  155...155 4.5 72 5 miRFP703 https://www.addgene.org/79988/ 674 703 90,900 8.6 127 Monomer 394 4.5 37 ...

  17. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...aftermath of the Supreme Court’s decision to ban gene patents in a case against Myriad, an organization...should come as no surprise that those of us at Addgene believe in the power of sharing, and especially...s how science progresses after all, with each generation of scientists standing on the shoulders of their...public database to gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly...And, last but not least (certainly not for us), Addgene launched this blog, A Better Way to Share Science...

  18. Editor's Choice, August 2016

    Type
    Blog Post
    ...Directed Mutagenesis by PCR Google Forums Round Up: First Impressions of NgAgo CRISPR Between the Genes: How...quick way to generate many mutated plasmid constructs. The second post was contributed by Addgene Scientist...month including a first of its kind for the Addgene blog - Addgene Software Engineer Kris Shamloo wrote a ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the...optimize and troubleshoot the system. Site Directed Mutagenesis by PCR & Single Base Editing with CRISPR For ...and discusses a newer, CRISPR-based technique to generate C->T or G->A transitions in a target sequence ...potential specificity issues, and other means of generating mutations in live cells are all discussed further...

  19. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ... technique, PCR primers specific to the 16s RNA genes of various commonly infecting mycoplasmas are used...The primers used in the process are either species/gene-specific or universal. This method is quick, efficient... 93–103 Additional resources on the Addgene blog Browse Addgene's viral vectors articles Find tips and...working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols ...being contamination free. The aerosols that are generated (while talking or pipetting) during working in...leading causes of contamination. These aerosols generated will enter the culture medium during subculturing...are used which trigger cellular mechanisms that generate luminescence. This can then be detected by a luminometer...

  20. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...line Y, or the phenotype of a mouse with a specific gene knock out, often you have to remember the experiment...Data Sharing Resources at Addgene.org Deposit Your Plasmids with Addgene Find Molecular Biology Protocols...innovation by all.  Additional Resources on the Addgene Blog Use Apps to Save Time and Track Your Experiments...
Showing: 661 - 680 of 1146 results