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We narrowed to 1,141 results for: Gene

Showing: 661 - 680 of 1141 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid...definitions (e.g. neurons defined by a single gene or projection). Intersectional expression approaches ... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions...cells based on a doubly-specified combination of genetic and/or anatomical-defined parameters, by placing...Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT recombinases...pAAV-CaMKIIa-Flpo None No Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...Vcre No Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...

  2. The Pleiades Promoter Project

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    Collection
    ... disorders by enabling region- and cell-specific gene-delivery in the mouse brain through these human ...List of Pleiades MiniPromoters MiniPromoter Source Gene Construct Reporter Negative Control N/A pEMS1312...

  3. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ...Prof Jim Collins and specialising in engineering gene regulation in yeast. Dr. Ellis is now one of the...Today’s episode of the Addgene Podcast features guest host and Addgene European Outreach Scientist, Benoit...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...it was a short journey for Benoit to interview Addgene Advisory Board Member and lead researcher at Imperial...Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest, Tom Ellis! Dr. Ellis...direct interactions between drugs and promoters of oncogenes. He returned to academic research in 2005 to investigate...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...

  4. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ... Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Senaratne is a graduate of the PhD program in Genetics and Genomics at Harvard Medical School, and is...is currently a fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

  5. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ... Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Senaratne is a graduate of the PhD program in Genetics and Genomics at Harvard Medical School, and is...is currently a fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

  6. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ...Use cloning grade DNA for direct cloning of your gene of interest into a plasmid backbone. Transfection...quantity and purity.  Additional resource on the Addgene blog Browse blog posts on plasmid cloning Read... conjugation, and transfection Resources on Addgene.org Read the molecular biology reference Find plasmids...

  7. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...protected. Effects of Activation of the PGC-1a1 Pathway Gene expression analysis of the animals’ skeletal muscles...protects the brain from depression. Mice that are genetically modified to produce extra PGC-1a1 only in their...psychiatric and cognitive conditions as well as neurodegenerative diseases.” I don’t know about you, but I think...right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC-1α1 modulates...

  8. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can skip this... perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do...Preparation of electrocompetent cells (protocol) At Addgene, we use the Mix & Go! E. coli Transformation Kit...large number of transformations being performed at Addgene, this adds up to a significant time savings. You...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...fluorescent biosensors to measure biomolecules or genes via FRET or other assays....specific biomolecules, the activity of specific genes and cellular processes, or other factors like environmental...mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial content, structure, stress and...for a sensor for a different analyte, a sensor for gene-specific activity, or for popular biosensors in ... Cells", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type PI Publication Return to Top ...also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids ...Gadella Calcium Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca(2+) indicator...

  10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...applications of fluorescent proteins, genetically encoded biosensors, and optogenetic tools. Follow her recent research...-the-art probes, many of which you can find at Addgene. Biliverdin chromophore Near-infrared fluorescent...FPs can be combined with blue-light controlled optogenetics, such as channelrhodopsins and tools based on...reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of a probe leads to less interference... Ref BphP-based FPs miRFP670 https://www.addgene.org/79987/ 642 670 87,400 14.0 198 Monomer  155...155 4.5 72 5 miRFP703 https://www.addgene.org/79988/ 674 703 90,900 8.6 127 Monomer 394 4.5 37 ...

  11. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...aftermath of the Supreme Court’s decision to ban gene patents in a case against Myriad, an organization...should come as no surprise that those of us at Addgene believe in the power of sharing, and especially...s how science progresses after all, with each generation of scientists standing on the shoulders of their...public database to gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly...And, last but not least (certainly not for us), Addgene launched this blog, A Better Way to Share Science...

  12. Editor's Choice, August 2016

    Type
    Blog Post
    ...Directed Mutagenesis by PCR Google Forums Round Up: First Impressions of NgAgo CRISPR Between the Genes: How...quick way to generate many mutated plasmid constructs. The second post was contributed by Addgene Scientist...month including a first of its kind for the Addgene blog - Addgene Software Engineer Kris Shamloo wrote a ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the...optimize and troubleshoot the system. Site Directed Mutagenesis by PCR & Single Base Editing with CRISPR For ...and discusses a newer, CRISPR-based technique to generate C->T or G->A transitions in a target sequence ...potential specificity issues, and other means of generating mutations in live cells are all discussed further...

  13. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ... technique, PCR primers specific to the 16s RNA genes of various commonly infecting mycoplasmas are used...The primers used in the process are either species/gene-specific or universal. This method is quick, efficient... 93–103 Additional resources on the Addgene blog Browse Addgene's viral vectors articles Find tips and...working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols ...being contamination free. The aerosols that are generated (while talking or pipetting) during working in...leading causes of contamination. These aerosols generated will enter the culture medium during subculturing...are used which trigger cellular mechanisms that generate luminescence. This can then be detected by a luminometer...

  14. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...elegant bacterial immune system and an efficient gene editing tool… but boy does it have a lot of parts... resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet...section of the bacterial chromosome that stores a genetic record of previously encountered foreign nucleic...looser PAM requirements, which are available from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from...CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence...sequence as the scaffold. gRNA: "Guide RNA" is a general term referring to the RNAs used by Cas proteins...Conclusion Ready for some more details? Check out the Addgene CRISPR guide or our CRISPR 101 ebook. We also ...

  15. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...line Y, or the phenotype of a mouse with a specific gene knock out, often you have to remember the experiment...Data Sharing Resources at Addgene.org Deposit Your Plasmids with Addgene Find Molecular Biology Protocols...innovation by all.  Additional Resources on the Addgene Blog Use Apps to Save Time and Track Your Experiments...

  16. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...this publication at Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence...multiple protein molecules (and also available from Addgene!) Since the signal level provided by SunTag is ...applicable to studies of cell cycle progression, epigenetics and cellular reactions to external stimuli. It...pantheon of useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References.... Find the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human.... Find the plasmids from this publication at Addgene. ...

  17. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... GFP-containing organism,” says Hanson, “using a gene encoding one of the unexpectedly photoconvertible... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...multiple important technologies, including FRET and optogenetics. Even though GFP has been so thoroughly characterized... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...

  18. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...approach is to target two exonic sites within a single gene using paired DSBs. This method allows for deletion...Medical School. Addgene is proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions...within the deletion boundaries may be produced to generate clonal allelic series. Single DSBs resolved by...biologists can imagine, create, and examine novel genetic configurations. Watch the following video to learn...the full protocol from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank... Medical School. His lab studies the molecular genetics of blood cell development and disease. Learn more...al. An erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin level. Science...

  19. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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    Blog Post
    ...they have been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling cascades...and Dr. Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR... to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents out ...  New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...CGE), which has been used in plasmid vaccine and gene therapy development to examine plasmid topologies...plasmid DNA concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: ...resources on Addgene.org  Addgene's Plasmids 101 eBookAddgene's Molecular Biology ReferenceAddgene's Restriction...length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to increasing affordability...RA, James AA, Kolodner R. recA-independent general genetic recombination of plasmids. Nature. 1981 Nov... performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut... the short-read Illumina MiSeq sequencing that Addgene uses.) However, when some researchers received ...
Showing: 661 - 680 of 1141 results