We narrowed to 772 results for: Mos

1. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   ...epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead modifiers...

2. Technologies Enabled by NanoLuc® Luciferase

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   Blog Post

   ...fluorescent protein. FRET can be measured by exciting the most blue-shifted fluorescent protein (donor) and measuring...

3. Plasmids 101: Restriction Cloning

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   Blog Post

   ...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...

4. Antibodies 101: Antibody Engineering and Directed Evolution

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   Blog Post

   ...traction in recent years. The goal is to engineer the most miniscule parts of life, like proteins, to study...

5. Antibodies 101: Flow Cytometry

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   Blog Post

   ...microscopy must be used. To learn more about the most suitable fluorescent microscopy technique for your...

6. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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   Blog Post

   ...expressing Cre. The result of a system like this is a mosaic of cells labelled with different fluorescent proteins...

7. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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   Blog Post

   ...Rather than reprogram cells all the way back to their most primitive pluripotent stem cell state, through transdifferentiation...

8. Cloning

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   Guide

   ...The following guide will highlight several of the most popular cloning methods used to create recombinant...method, and for many reasons, remains one of the most popular today. Restriction enzymes, which are naturally...

9. Optogenetics + CRISPR, Using Light to Control Genome Editing

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   Blog Post

   ...photoactive Cas9 tool, as shown in the figure below. Their most successful design utilized Magnet photoswitchable...

10. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post

    ...four adenine base editors (ABEs). ABE7.10 is the most active editor, displaying an average editing efficiency...

11. Modular Cloning Guide

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    Guide

    ...constructs for integration into Y. lipolytica chromosomal loci. Yeast GPCR-sensor Toolkit Yeast Expression...

12. Optogenetics Guide

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    Guide

    ...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...