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We narrowed to 1,025 results for: URE

Showing: 91 - 120 of 1025 results

  1. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...registration please go to http://futureofresearch.org/boston/. The Future of Research Symposium A year ago...daily lives and future prospects. Among the topics discussed, many related to the structure of the biomedical... overall structure of the biomedical research sector, and what a tenable workforce structure may look ...biomedical graduates take in their future careers. To participate in the Future or Research Symposium Boston...The second Boston Symposium on the Future of Research will be held from 22-24 October. This blog has ...has been contributed by guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here... of the six panel discussions to be held at the Future of Research Symposium Boston 2015 – Academic Data...

  2. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...upcoming "Future of Research" conference. You can register for the conference here. The Future of Research...colleagues in envisioning the Future of Science. Attend the Symposium on the Future of Science: A postdoc-...science to be happy. What do we have to change to ensure that all scientists can have successful careers...postdoc-organized symposium on the future of the scientific endeavor October 2-3, 2014 Boston University Jacob...

  3. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...protein variants. The future of DNA feature identification What’s next for common features? We plan to continue...creating a database of common features, and devising rules for identifying a feature even when the match is ...is imperfect. SnapGene's feature database The source of common features was our collection of popular ...identity when detecting a reference feature. For a coding sequence feature that may be used to make fusion... into the original literature, some of it decades old, to provide reliable feature annotations. Even with...that a feature is missing from our database, or if you see an opportunity to harness common feature detection... planning, visualizing, and documenting their procedures. Preventable errors in the design of cloning ...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... Elite: Nobel Laureates in the United States. Free Press. New York. Resources MDH CUREs Community (MCC...number of honorees to have trained under a previous laureate (Zuckerman, 1977; Ma et al., 2020). Many have ...trainees at an earlier point in their careers? A CURE for Biochemistry Undergraduate biochemistry courses... Course-based Undergraduate Research Experience (CURE). Having incorporated a discovery-based lab on planarian... a stable, easily purified protein with easily measured activity, MDH is particularly amenable to in-class...    Fig. 1: Undergraduates in Provost’s CURE prepare to analyze their recently purified MDH to...the impact of phosphorylation on the enzyme’s structure, function, and interaction with other proteins...

  5. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...takeaways I got from the symposium. The goal of the Future of Research Symposium On the FOR website, the FOR...prevent us from identifying problems with the structure of the academic research workforce, and we are...only 14.3% of PhDs in the life sciences end up in tenure track position 5 years after receiving their PhDs... needs to be asked going forward is: How do we measure inclusion and equity? This is a difficult question...capturing, quantifying, and preventing this type of failure. Final thoughts The FOR Symposium Boston 2015 shined...Stephan’s Book - How Economics Shapes Science The Future of Research Website Additional Publications of ...

  6. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...office space on the nicely manicured lawn of University Park at MIT.  I’m sure at some companies, employees...those early years, the founders knew that company culture and enjoying your job were important parts of team... team buidling - they relieved some of the pressure of start up life and maintained their sanity by throwing... still isn't time for goofing off, the company culture here at Addgene has remained paramount. Candidates...Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best place to work, we do SO many...over 60 people join the fun - including a lot of future Addgenies as shown in the photo below.  Kids and... was another successful event! Addgene company culture At Addgene we organically developed the philosophy...

  7. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...relief that I attended the amazing, postdoc-driven Future of Research Conference at Boston University last...postdocs are taking some steps to control their own future in science (see my previous blog on this).  Indeed...writing about issues in our science training infrastructure for some years.  He spoke eloquently and vehemently... the need to pay postdocs more.  What will the future of research be? The second day of the conference...conference organizing team.  The topics were Training, Structure of the Workforce, Metrics and Incentives and Funding...beginnings of solutions can’t come too soon.  Watch the Future of Research Website for news and updates on the...

  8. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...power of light. In a recent article published in Nature Methods, Boyden and his team describe the discovery... or pathology, and by disabling neurons you can figure out what they are necessary for. We published our...branch on the tree of life. Our most recent paper in Nature Methods offers the first true two-color pair. We'll... to see how a monkey changes decision making, to cure a human patient. We can't do it all by ourselves...Distinct Neural Populations. Nathan Klapoetke et al. Nature Methods. 2014 Feb 9. (PubMed). Read More: Other...

  9. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...FPs to highlight various subcellular structures. Please see Figure 1 for commonly used markers for major...up of organs, cells comprise compartments and structures, called organelles. Take a sneak peak inside ...fluorescent protein fusions to visualize subcellular structures. For example, you might use your fluoresent protein...organelle of interest. Click on the gene names in the figure below to find plasmids containing that particular...find more plasmids for labeling your subcellular structure of interest in the Allen Institute for Cell Science...possible to get an idea about the subcellular structures your protein resides in and potentially which...proximity of two proteins within the same subcellular structure or protein complex. By coexpressing both the marker...

  10. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...science in D.C. "Scientists shouldn't be hidden figures.  Ask me about my science!", read my sign.  People...University. She studies beta-cell function and failure in Type 2 Diabetes. Birmingham, AL, USA 33.5207...data collection, destroys existing data, and guts future funding.I marched because science belongs to the... some little scientists (as you can see in the picture) and this march proved to be the perfect opportunity... a Scientific Instrument Maker at Intellectual Ventures Laboratory. Vancouver, BC Canada 49.2827°N 123.1207...attended the march in Vancouver, BC, although I wasn’t sure I’d go until several hours before it started. It...Previow Post on the March for Science Learn How Future of Research is Advocating for Change Within the...

  11. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...with the literature There are a few ways to make sure you are keeping up with the literature in your field... with the scientific literature a little bit at a time. Searching for literature My go-to search engines...the literature until you have all of the information you need. Reading primary research literature The...papers are also just poorly written. By nature, scientific literature is information dense and since it continually...you daily updates with relevant literature. Organizing the literature As a scientist, you will begin to...require numerous other references to scientific literature to substantiate the facts upon which it builds...means you have to become familiar with a body of literature related to the topic. Finding reliable references...

  12. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... manipulate biology based on molecular features found in nature, with the ultimate goal to improve the...impact on this challenge either for magnetic capture or for capture using microfluidic devices. Addgene: What...antibodies against common tumor markers to subsequently capture cancerous cells expressing low levels of tumor ...binding stability really mattered for efficient capture. We wanted to be able to build a highly multivalent...for keeping going while we worked out the cell capture system.) Cancer and other applications Addgene:...technology mean in the context of cancer? Howarth: The capture of circulating tumor cells (CTCs) is going to have...treatment of cancer. It is clear that one needs to capture a diverse range of CTCs to cope with the variation...

  13. CRISPR Guide

    Type
    Guide
    ...addition of a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased ...synthesized (Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries...Berkeley. Plan Your CRISPR Experiment Resources Figures All figures were created using BioRender.com (Link opens..., N., Ishitani, R., Zhang, F., & Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA...vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. PMID: 25830891 Rauch...became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism Engineered...gRNA left free to interact with the target DNA. Figure 2: Overview of the NHEJ repair mechanism Cas9 will...

  14. Antibody Guide

    Type
    Guide
    ...isolated and placed in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is...Antibodies can also be produced in cell cultures using hybridomas, cell cultures created by fusing B cells with...form a “Y” shape, as shown in Figure 1. The two arms of the Y structure are responsible for binding the...Monoclonal antibodies are generated from cultured B cells, where each culture should produce the same antibody...Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared... a signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary ...In a sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest...

  15. Lentiviral Vector Guide

    Type
    Guide
    ...Table 1. Figure 4: Third-generation lentiviral plasmids. Created with BioRender.com. Feature Second-generation... chromatin architecture through large-scale mapping of lentiviral integration sites. Nature Protocols ...to continue their lifecycle and replicate. This feature can be harnessed for stable delivery of various... typically ranges from 8–10 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...have been removed or mutated for increased safety. Figure 1: Wild-type lentivirus genome. Created with BioRender.com...largely intact. First-generation plasmids include (Figure 2): Transfer plasmid — contains transgene and wild-type...envelope removed Envelope plasmid — contains env Figure 2: First-generation lentiviral plasmids. Created...

  16. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...replication and also act as signals for packaging. Figure 1: Wild-type AAV genome. Created with BioRender.com...do not trigger a strong immune response. These features have made AAVs the preferred choice to be used...components, see the AAV Plasmid Elements table . Figure 2: Plasmids required for rAAV vector production...methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid is compatible with...available in your lab. When cloning your plasmids, be sure to use recombination deficient bacterial strains... the ITRs found in transfer plasmids. This will ensure that the repeats will be maintained and often results...and instead provided by the chosen cell line to ensure safety and prevent the potential formation of replication-competent...

  17. Adenovirus Guide

    Type
    Guide
    ...Collection page . Figure 5: Overview of AdEasy™ adenoviral vector production system. Figure is based on the...assemble icosahedral capsids and build new virions. Figure 1: Wild-type adenovirus and its genome. Genes over...which incorporate additional genomic deletions. Figure 2: First-generation rAdV vectors. Created with ...using first- and third-generation rAdV vectors. Figure 3: Second-generation rAdV vectors. Created with... challenge of the HCAdV vector production is to ensure no carryover from the HV in final viral preparations... genome or using other plasmids instead of HVs. Figure 4: Third-generation rAdV vectors and helper virus...methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid is compatible with...

  18. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...to continue their lifecycle and replicate. This feature can be harnessed for stable delivery of various...gamma-retroviruses ranges from 9–11 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus genome. Created with...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA...infectivity for different cell types (known as tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com...methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid is compatible with...available in your lab. When cloning your plasmids, be sure to use recombination-deficient bacteria strains,...

  19. Optogenetics Guide

    Type
    Guide
    ...Guide to Channelrhodopsin Variants: Features, Limitations and Future Developments. Exp Physiol. 96(1): ...optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons) and their...optogenetics research utilize light to detect, measure, and control molecular signals and cells in order...functions. Example schematic of an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed... allow the fast depolarization of neurons upon exposure to light through direct stimulation of ion channels... in excitation (depolarization) of the neuron. Feature enhancements include: Increased photocurrent amplitude...from the cryptophyte Guillardia theta ). Other feature enhancements include: Increased photocurrent amplitude...

  20. Plan Your Experiment

    Type
    Guide
    ...interest is PCR amplified, PCR products are denatured-renatured, treated with a nuclease that cleaves DNA...reagents are appropriate for a given experiment. Make sure to check whether reagents are available to carry... gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then delivered to...match the target locus, but it is also critical to ensure that the gRNA target sequence does NOT match additional...allele may be different owing to the error-prone nature of NHEJ. In HDR gene editing experiments, most ...protein-based genome editing in vitro and in vivo. Nature Biotechnology . 33 (1), 73–80. PMID: 25357182...

  21. Chemogenetics Guide

    Type
    Guide
    ...check out our Chemogenetics Plasmid Collection . Figure 1: General overview of a chemogenetics experiment...muscarinic receptor and has not been used in vivo . Figure 2: DREADDs, their ligands, and signaling properties...and/or K + ions, and activates neuronal activity. Figure 3: PSAM4-based ligand-gated ion channels, their...optogenetic systems in our Optogenetics Guide . Figure 4: Luminopsins can be activated through chemogenetic... control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or... X, Stone O, Roth BL, Jin J (2015). The First Structure–Activity Relationship Studies for Designer Receptors...

  22. Molecular Biology Reference

    Type
    Guide
    ...propagation of these plasmids through cell division. To ensure the retention of plasmid DNA in bacterial populations.... Under these conditions, there is a selective pressure to retain the plasmid DNA, despite the added replication...organisms, including plants, worms, mice, and even cultured human cells. Although plasmids were originally...bacteria, but also provide those bacteria with a pressure to maintain and replicate your plasmid over multiple... extremely important when verifying plasmids to ensure each plasmid contains the essential elements to...replicated using the modified bases and a microscope captures the fluorescent color that is emitted each time...

  23. Science Guides

    Type
    Guide
    ...conjugated to a signaling molecule, can be used to capture, quantify, and identify proteins of interest. Read...Optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons) and their...optogenetics research utilize light to detect, measure, and control molecular signals and cells in order...

  24. Addgene's eBook Collection

    Type
    Guide
    ...When you're not sure where to start or need a handy reference, Addgene's eBooks are here to help!...Addgene's eBook Collection When you're not sure where to start or need a handy reference, Addgene's eBooks...you need to know about plasmids, from parts, nomenclature, and uses to common promoters and cloning techniques...

  25. Guide to Using Pooled Libraries

    Type
    Guide
    ...infected cell receives only one plasmid. To make sure that every plasmid is adequately represented in ...planning to conduct a pooled library screen. The figure above is an outline of how a gRNA library is used...cells that survive post-selection. The selective pressure must be strong enough that most of the cells die...

  26. Promoters

    Type
    Guide
    ...the TSS. Eukaryotic promoters are so complex in structure that the DNA tends to fold back on itself which...which is a main component of a cell’s ribosome structure. Ribosomes are the site of protein syntehsis where...

  28. Cloning

    Type
    Guide
    ...protocol on our website. Ligation Independent Cloning (Figure adapted from LIC protocol ) Back to Top Yeast-mediated...

  29. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...crystallography cannot capture flexible or disordered regions, so crystal structures like those in Figures 2 and 3 don...biology! Major features First, we’ll cover the basic parts of the Cas9 enzyme.   Figure 1: A cartoon...from Streptococcus pyogenes.   Figure 2:  Crystal structure of S. pyogenes Cas9 in the apo state...state that it doesn’t appear in the crystal structure (Figure 2) at all. When this crucial recognition region...learn about this process!      Figure 3: Crystal structure of S. pyogenes Cas9 bound to guide RNA...are disordered and don’t appear in the Figure 3 crystal structure, reflecting the less stringent recognition...tolerate mismatches in this region. The crystal structure in Figure 3 shows the guide RNA stabilizing the DNA-binding...

  30. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...contamination of the rest. Avoid long exposure of the cell culture to air and make sure to tighten the flask cap before...contamination in your cell culture or you are about to begin a new cell culture project. If mycoplasmas ...They are present in most of the cell culture facilities, tissue culture labs and every cell culturist has... every cell culture. Reusing the same bottle of serum again and again for each subculture can enhance ...does not ensure complete sterilization as mycoplasma can even escape 0.2-micron filters. Figure 1: Contamination...seen in the culture, it is a good option to discard the flasks (unless the source of culture is irreplaceable... to be 100% sure. Before testing for mycoplasma, the cells should be in continuous culture for at least...
Showing: 91 - 120 of 1025 results