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We narrowed to 189 results for: lentiviral

Showing: 121 - 160 of 189 results

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...the authors packaged it into lentivirus and used the resulting lentiviral library to generate a population...Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral CRISPR library     CRISPR pooled libraries enable...Library. This complex second-generation CRISPR lentiviral library targets nearly all human protein-coding...from the CDC and cloned into a third generation lentiviral transfer vector. Each gene has been FLAG-tagged...applications. First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed...dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing... system has a much larger cargo capacity than lentivirus and is capable of efficiently creating stable...

  5. Addgene: The First Twenty Years

    Type
    Blog Post
    ...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing...

  8. CRISPR Library Amplification

    Type
    Protocol
    ...gel ( see restriction digest protocol here ). Lentiviral plasmids can recombine between their LTRs resulting...these sequences are not efficiently packaged into lentiviral particles. See below for options if the recombinant...

  9. Pooled Library Amplifications

    Type
    Blog Post
    ...difficult to work with. For example, a library in a lentiviral backbone might be more prone to recombination...

  11. Hot Plasmids February 2024

    Type
    Blog Post
    ...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...proportions from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). ...

  15. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...this gene can be targeted to RPE cells using a lentiviral vector and a clinical trial is ongoing. Moving...other viral vectors such as adenovirus (AdV) and lentivirus (LV) are known to transduce retinal cells, AAV...

  16. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same ...same family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...

  22. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...been used - GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the...

  27. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

  28. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...safety. For a summary of all lentiviral plasmid components, see the Lentiviral Plasmid Elements table . In...different generations. Lentiviral Generations First-generation First-generation lentiviral plasmids are largely...Second-generation Second-generation lentiviral plasmids greatly improved the safety of lentiviral vector production by...between second- and third-generation lentiviral plasmids. Lentiviral Vector Production Cloning Cloning your...

  29. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...

  30. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Clear Filters Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson...cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore...Therefore, you must be equipped and authorized to use lentivirus in your lab. Once you have determined that CRISPR-based...

  32. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...gamma-retroviruses may not be efficiently packaged by lentiviral packaging systems, and vice versa. Gamma-retroviral... 293T (HEK293T) cells (Figure 3A), similar to lentiviral packaging methods . The three plasmids described...BioRender.com. Viral Vector Integration Much like lentiviral vectors, gamma-retroviral vectors integrate at...to make stable cell lines. Like their popular lentiviral counterparts, gamma-retroviral vectors offer ...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...cell line protocol . Addgene’s protocol is for lentiviral vectors, but a similar method can be applied ...

  33. Guide to Using Pooled Libraries

    Type
    Guide
    ...cells of interest. With others, notably pooled lentiviral plasmid libraries, the plasmids must first be...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

  34. Plan Your Experiment

    Type
    Guide
    ...high efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...

  35. CRISPR Guide

    Type
    Guide
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147...

  36. Molecular Biology Reference

    Type
    Guide
    ...these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that...viral service offers select ready-made AAV and lentiviral particles. Visit our viral service page to learn...

  38. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...humans, earning this virus a BSL-2 classification. Lentivirus (a type of retrovirus) and gamma retrovirus require...and required in BSL-2 labs. When working with lentivirus, many organizations require or recommend disposable...you have to work with BSL-2 materials, such as lentivirus. Most of this work will need to be done in a ...that particles are not sprayed into the air. For lentivirus and other BSL-2 materials, you’ll need to use...for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge bin covers, which...

  39. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...different types of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results...effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once thawed, there was... 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps Additional Resources on Addgene.org Check... lentivirus...

  40. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Mammalian Lentivirus pRDA_512 (RSV-driven; lentiGuide-based) Mammalian Lentivirus pRDA_722...lentiCRISPRv2-based; preferred over 512) Mammalian Lentivirus pRDA_789 (CMV-driven; CROPseq) Mammalian...Organism (Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector...
Showing: 121 - 160 of 189 results