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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,005 results for: Eras

Showing: 61 - 80 of 1005 results

  1. Promega Plasmid Collection

    Type
    Collection
    ...HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter... two proteins interact, the subunits come together to form an active enzyme and generate a bright luminescent...NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...solutions that help advance science while reducing operational complexity. This unique collection contains plasmids...experimental workflows. The collection contains luciferase reporter vectors for sensitive gene expression..., reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other...

  2. Antibody Guide

    Type
    Collection
    ...signaling molecules are horseradish peroxidase (HRP) and fluorophores. Horseradish peroxidase (HRP) HRP ...with several suitable antibodies; if they all meet your criteria, you may either purchase several to compare...epitopes on one antigen. Monoclonal antibodies are generated from cultured B cells, where each culture should...variability. Other Affinity Reagents There are several alternatives to antibodies, ranging from antibody... aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles. Here are a few tips for storing...a chemiluminescent reaction in the presence of several different substrates. The luminescence produced...Applications Antibody-based applications can be generally classed into four categories: Quantification -...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...sequence and eliminating interactions with endogenous factors. Experimental Considerations Expression System ...relative ratio. Different generations of transactivators and promoters are generally cross-compatible. Your...transcriptional and proliferative state of each cell in a population. Proliferation history is monitored...Tetracycline Off Tetracycline On Experimental Considerations Tet Plasmids Inducible and repressible promoters... the tetracycline repressor protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA...other tet- or dox-dependent promoters (sometimes generally called Ptet) have been designed by combining tet...than simply removing the repressor. 2nd and 3rd Generation Systems Subsequent efforts have improved the ...

  4. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your ...phosphoserine into the growing polypeptide chain to generate the new phosphorylated protein. Protein Phosphorylation...phosphoprotein yield, rEcoli growth characteristics, and an overall improved ease of use when expressing recombinant...further details in Mohler et al. (2023) and find general tips on phosphoprotein expression in rEcoli XpS...Barber et al., 2018). This phosphosite library was generated in a single mixed pool by expressing and purifying...assessments. The Mode #1 phosphosite library can be generated with either phosphoserine using SepOTSλ (Plasmid...screening phosphorylation-dependent protein-protein interactions Figure 2: Mode #1 and Mode #2 phosphosite library...

  5. Lentivirus Plasmids

    Type
    Collection
    ...used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253...transgene expression. There are some safety considerations to evaluate when working with lentivirus: these...potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems available at Addgene have ...information about: Lentiviral components 2nd versus 3rd generation lentiviral systems Viral production Frequently...produce an infectious viral particle (a virion) are generally divided among multiple plasmids. Some plasmids... Packaging Contains Rev; can be used with 3rd generation transfer plasmids along with additional packaging...Packaging Contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding...

  6. Rett Syndrome

    Type
    Collection
    ...curated literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information...MECP2 reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to...contrast to the sustained loss of skills in neurodegenerative conditions, the regressive period in Rett ...locally or globally. The MECP2 protein contains several functional domains, including: the N - t erminal...acid and Thyroid hormone receptor (NCoR/SMRT) interacting domain (NID) a nuclear localization signal (not...the MECP2 protein in mouse and rat models have accelerated our understanding of Rett syndrome at the molecular...They are also an important tool for developing therapeutic strategies to treat Rett syndrome and restore...

  7. Brain Initiative Collection

    Type
    Collection
    ...the revolutionary dynamic picture of the brain generated through this effort will provide novel ways to...Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111067-AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111068-AAV1 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2...promoter Lin Tian 111068-AAV5 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2...promoter Lin Tian 111069-AAV1 pAAV-CAG-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.1...

  8. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2... vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...poorly understood but are critical for developing therapeutic strategies to prevent viral spread. As a Biosafety...the live virus. Alternatively, it is possible to generate pseudovirus, where the structural and functional...spike protein is inefficient for pseudotyping; several modifications may enhance infectious particle production...lists expression plasmids that are suitable for generating pseudotyped virus for SARS-CoV-2 and other coronaviruses...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EGFP Ken-Ichi Takemaru 54234 mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson 54491 mCherry-Lifeact...Vladislav Verkhusha 79990 pKeratin-miRFP703 Intermediate Filaments (epithelial) Keratin miRFP703 Vladislav Verkhusha...mCherry Alpha Yap 54219 mEmerald-Paxillin-22 Focal Adhesions paxillin mEmerald* Michael Davidson 15233...GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin mEmerald* Michael Davidson 79997...EEA1 GFP Silvia Corvera 42635 TagRFP-T-EEA1 Early endosomes EEA1 TagRFP-T Silvia Corvera 38770 pEF.myc....Optogenetics Fluorescent Protein Blog Posts Background General Cytoskeletal Junctions Secretory Pathway Background...structures as these well-characterized proteins. General Structures The cell contains a variety of organelles...

  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide...Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-gTEMP Intracellular Heat...You may also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics...family Cameleon calcium sensors Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an... cAMP levels for imaging in live cells Fourth-generation epac-based FRET sensors for cAMP feature exceptional...GMP) Green fluorescent probe for cGMP (cGull) Generation of a cGMP Indicator with an Expanded Dynamic ...Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe. Nat...

  11. Genetic Code Expansion

    Type
    Collection
    ...the new amino acid is introduced in the media. Generally, you will need two plasmids, as depicted in the...interest: The non-canonical amino acid, which is generally introduced in the media. A new codon to be allocated...in selected amino acids within a protein, any alterations in structure or function in the protein can be...and evolve proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning...beginning to reprogram the genetic code, there are several things to consider. If you are working off of a...tRNA pair instead. For directed evolution of the liberated EcTrpRS/tRNA pair to incorporate other Uaas (derived...

  12. Zebrafish Plasmid Collection

    Type
    Collection
    ...developing embryos. zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R ...(embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding, ...study of cancer, inflammation and infection, regeneration, human neurological disorders, microbiota studies...CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the zebrafish... Vivo Lineage Tracing during Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral...abundance, size, and localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly...Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ...estrogen receptor 1 DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1 ESR2 estrogen receptor 2 (ER beta) ER-BETA...MGC3886 ERAP1 endoplasmic reticulum aminopeptidase 1 A-LAP, ALAP, APPILS, ARTS-1, ARTS1, ERAAP, ERAAP1, KIAA0525...disulfide isomerase family A, member 2 PDA2, PDI, PDIP, PDIR PDIA3 protein disulfide isomerase family A...KGFLP1 keratinocyte growth factor-like protein 1 MGC125746, MGC125747, MGC126891 KGFLP2 keratinocyte growth... TDGF1 teratocarcinoma-derived growth factor 1 CR, CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived...systems. Upon infection, the immune system triggers several processes to activate cells to recognize and get...the dominant defense strategy during the first several days following exposure to a pathogen. The innate...

  14. COVID-19 Resources

    Type
    Collection
    ...suitable for generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent...mammalian sequences linked to SARS-CoV-2 infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse...top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as having ... our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK and DETECTR - Simple, portable...window) reverse transcriptase, protease, and Bst polymerase constructs now available from Andrea Pauli's ...nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2. Huo, et al. Nat Struct Mol Biol. 2020...Diagnostic Toolkit Enzymes. (unpublished) Taq polymerase and MMLV reverse transcriptase expression plasmids...

  15. Plant Plasmids and Resources

    Type
    Collection
    ...plant expression vectors containing several cassettes and generate multi-construct transgenic plants -...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use...includes plasmids containing promoters that drive general and species-specific gene expression (see the column...CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the Plant ...selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner...MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes...publications are created. Papers from the plant biology literature are discussed with one of the authors. The podcast...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression mEmerald 487 509 39 6 Monomer (A206K) mEmerald-N1 - Mammalian Expression mEmerald-C1 - Mammalian...Expression mPA-Emerald 487 509 - Monomer (A206K) mPA-Emerald-N1 - Mammalian Expression mPA-Emerald-C1 - Mammalian...HisD-rsTagRFP - Bacterial Expression rsEmerald 491 508 rsEmerald-pBAD - Bacterial Expression rsGFP1 493...Expression Species . The content for this page was generated with the help of Erik Snapp . Empty Backbones ...Mammalian Expression mEmerald-pBAD - Bacterial Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-...

  17. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...full-text of the publication here . Article Citation Generation of Genomic Deletions in Mammalian Cell Lines ... incubation time allows for sufficient cell proliferation for screening genomic DNA (gDNA) for the intended... and 7.1 ). The bulk cells have sufficiently proliferated when the concentration exceeds ~100,000 cells...80% confluence. The clones have sufficiently proliferated once macroscopically visible with ~2 mm diameter...pellets in 50 μl of DNA extraction solution. NOTE: Generally ~100,000 cells are used for DNA extraction, although... band” in separate reactions. NOTE: Numerous polymerases may be used for step 6.3 . Run samples in a thermocycler...biallelic deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning...

  18. TALEN Plasmids and Kits

    Type
    Collection
    ...accessory plasmids available from Addgene for generating your own specific TALE plasmids.... TALENs assembled by Golden Gate. Validated in several species. Takashi Yamamoto Additional Plasmids for... Gate TALEN kit (Bogdanove/Voytas) in order to generate TALORs (TAL Orthongal Repressors) that can be ...Grunwald pCS2TAL3-DD and pCS2TALE3-RR are next generation TALEN backbone vectors designed for use with ...segments (136AA and 63AA, respectfully). This next generation architecture has been shown to increase mutation... C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN(Sangamo)-Destination.... In addition, a T7 promoter was included for generating TALEN mRNAs through in vitro transcription as...

  19. mTOR Pathway

    Type
    Collection
    ... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer promotes the conditions...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...specific relationship. The content for this page was generated with the help of David Sabatini . mTOR Pathway...specific relationship. The content for this page was generated with the help of David Sabatini . Return to top...

  20. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular ... stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited here with Addgene...With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular ...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...164500 POLR2A-mEGFP AICSDP-117 mEGFP RPB1 RNA polymerase II 168798 TERF2-mEGFP AICSDP-77 mEGFP Telomeric...CTCF-binding sites 170818 PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication ...Protocols The Allen Institute for Cell Science has several protocol videos available that may be helpful for...
Showing: 61 - 80 of 1005 results