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We narrowed to 1,000 results for: Eras

Showing: 61 - 80 of 1000 results

  1. Rinehart Lab Phosphoprotein Reagents

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    Collection
    ...The Rinehart Lab has developed several tools useful for the incorporation phosphoserine into your gene...phosphoprotein yield, rEcoli growth characteristics, and an overall improved ease of use when expressing recombinant... in Mohler et al., Mol Syst Biol 2023 and find general tips on phosphoprotein expression in rEcoli XpS... Biotech. 2018). This phosphosite library was generated in a single mixed pool by expressing and purifying...assessments. The mode #1 phosphosite library can be generated with either phosphoserine using SepOTSλ ( 68292...screening phosphorylation-dependent protein-protein interactions. The phosphosite DNA library can also be introduced...of phosphorylation-dependent protein-peptide interactions (“mode #2” expression, Barber et al, Nat. Biotech...

  2. Viral Vectors

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    Collection
    ...and generate virus (right), similar to how natural viruses can be delivered to a host and generate virus... Adenovirus vectors for gene therapy, vaccination and cancer gene therapy. Curr Gene Ther. 2013 Dec;13...can direct long-term transgene expression, but generally are not permanently integrated into the host genome...oncolytic) adenoviruses are utilized for cancer therapy because of their ability to preferentially infect...can be used to target specific cells or tissues. Overall, viruses can efficiently get genetic material into...an organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered ...genetic material (termed the viral genome) that, in general, encodes for more viruses. When a virus infects...

  3. Bacterial Expression Systems

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    Collection
    ...to measure protein-protein interactions; if two proteins of interest interact in a bacterial two-hybrid...pTac GFP, Luciferase (LuxCDABE) Attila Karsi Broad host range vectors for expressing luciferase and fluorescent...to a particular sequence? Do these two proteins interact? What happens when I overexpress this enzyme at... redirecting metabolic pathways to produce a therapeutic compound, there are many different ways to alter...appropriate construct, grown at the permissive temperature (32°C) to allow transposition, and the construct... then cured by growing at the non-permissive temperature (42°C). Transposition is efficient enough that...about your protein’s function, structure, and interactions! Plasmid ID Promoter Epitope Tag(s) ID Purpose...

  4. Lentivirus Plasmids

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    Collection
    ...used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253...transgene expression. There are some safety considerations to evaluate when working with lentivirus: these...potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems available at Addgene have ...information about: Lentiviral components 2nd versus 3rd generation lentiviral systems Viral production Frequently...produce an infectious viral particle (a virion) are generally divided among multiple plasmids. Some plasmids... Packaging Contains Rev; can be used with 3rd generation transfer plasmids along with additional packaging...Packaging Contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding...

  5. Plan Your Experiment

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    Collection
    ...want to: Generate complete and permanent loss of gene expression or function ( knockout )? Generate a specific... Edit Generate a specific user-defined sequence change in a particular gene, such as generating a point...like never before. This section will provide a general framework to get you started using CRISPR in your...Manipulation Application Cas9 gRNA Additional Considerations Knockout Permanently disrupt gene function ... enrich positive cells, or selection marker to generate stable cell lines (for details, see Plasmids 101...inserted into an AAV transfer vector and used to generate AAV particles (for details, see our AAV Guide ...Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then delivered...

  6. Rett Syndrome

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    ...curated literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information...MECP2 reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to...contrast to the sustained loss of skills in neurodegenerative conditions, the regressive period in Rett ...locally or globally. The MECP2 protein contains several functional domains, including: the N - t erminal...acid and Thyroid hormone receptor (NCoR/SMRT) interacting domain (NID) a nuclear localization signal (not...the MECP2 protein in mouse and rat models have accelerated our understanding of Rett syndrome at the molecular...They are also an important tool for developing therapeutic strategies to treat Rett syndrome and restore...

  7. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2... vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...poorly understood but are critical for developing therapeutic strategies to prevent viral spread. As a Biosafety...the live virus. Alternatively, it is possible to generate pseudovirus, where the structural and functional...spike protein is inefficient for pseudotyping; several modifications may enhance infectious particle production...lists expression plasmids that are suitable for generating pseudotyped virus for SARS-CoV-2 and other coronaviruses...

  8. Brain Initiative Collection

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    Collection
    ...the revolutionary dynamic picture of the brain generated through this effort will provide novel ways to...Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111067-AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111068-AAV1 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2...promoter Lin Tian 111068-AAV5 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2...promoter Lin Tian 111069-AAV1 pAAV-CAG-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.1...

  9. Fluorescent Protein Guide: Subcellular Localization

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    Collection
    ...EGFP Ken-Ichi Takemaru 54234 mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson 54491 mCherry-Lifeact...Vladislav Verkhusha 79990 pKeratin-miRFP703 Intermediate Filaments (epithelial) Keratin miRFP703 Vladislav Verkhusha...mCherry Alpha Yap 54219 mEmerald-Paxillin-22 Focal Adhesions paxillin mEmerald* Michael Davidson 15233...GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin mEmerald* Michael Davidson 79997...EEA1 GFP Silvia Corvera 42635 TagRFP-T-EEA1 Early endosomes EEA1 TagRFP-T Silvia Corvera 38770 pEF.myc....Optogenetics Fluorescent Protein Blog Posts Background General Cytoskeletal Junctions Secretory Pathway Background...structures as these well-characterized proteins. General Structures The cell contains a variety of organelles...

  10. Fluorescent Protein Guide: Biosensors

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    Collection
    ...highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide...Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-gTEMP Intracellular Heat...You may also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics...family Cameleon calcium sensors Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an... cAMP levels for imaging in live cells Fourth-generation epac-based FRET sensors for cAMP feature exceptional...GMP) Green fluorescent probe for cGMP (cGull) Generation of a cGMP Indicator with an Expanded Dynamic ...Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe. Nat...

  11. Genetic Code Expansion

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    ...the new amino acid is introduced in the media. Generally, you will need two plasmids, as depicted in the...interest: The non-canonical amino acid, which is generally introduced in the media. A new codon to be allocated...in selected amino acids within a protein, any alterations in structure or function in the protein can be...and evolve proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning...beginning to reprogram the genetic code, there are several things to consider. If you are working off of a...tRNA pair instead. For directed evolution of the liberated EcTrpRS/tRNA pair to incorporate other Uaas (derived...

  12. Zebrafish Plasmid Collection

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    ...developing embryos. zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R ...(embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding, ...study of cancer, inflammation and infection, regeneration, human neurological disorders, microbiota studies...CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the zebrafish... Vivo Lineage Tracing during Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral...abundance, size, and localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly...Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting...

  13. Cre-lox system

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    Collection
    ...expression when cloning in E. coli . This alteration enables the generation of a single vector containing Cre ...molecules (in trans), a translocation event is generated between the target sites. For a more thorough ...Drosophila. Read Addgene's Blog on Cre alternatives from Gerald Rubin's lab. Cre-containing Plasmids Scientists... Cre-EGFP fusion in a promoterless vector with several available restriction sites none Mammalian Sauer... region including the polyadenylation site and several introns tet-hCMV Mammalian Sauer 12168 pMB80 (R26...hsp70 Insect Rubin 32606 Ttr:Cre Cre expressed in visceral endoderm MCK Mammalian Hadjantonakis 33340 LGmCreER...backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre, luciferase, and sgRNA expression EFS AAV Zhang 60229 AAV...

  14. Immunology Research Plasmids and Resources

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    Collection
    ...estrogen receptor 1 DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1 ESR2 estrogen receptor 2 (ER beta) ER-BETA...MGC3886 ERAP1 endoplasmic reticulum aminopeptidase 1 A-LAP, ALAP, APPILS, ARTS-1, ARTS1, ERAAP, ERAAP1, KIAA0525...disulfide isomerase family A, member 2 PDA2, PDI, PDIP, PDIR PDIA3 protein disulfide isomerase family A...KGFLP1 keratinocyte growth factor-like protein 1 MGC125746, MGC125747, MGC126891 KGFLP2 keratinocyte growth... TDGF1 teratocarcinoma-derived growth factor 1 CR, CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived...systems. Upon infection, the immune system triggers several processes to activate cells to recognize and get...the dominant defense strategy during the first several days following exposure to a pathogen. The innate...

  15. COVID-19 Resources

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    ...suitable for generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent...mammalian sequences linked to SARS-CoV-2 infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse...top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as having ... our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK and DETECTR - Simple, portable...window) reverse transcriptase, protease, and Bst polymerase constructs now available from Andrea Pauli's ...nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2. Huo, et al. Nat Struct Mol Biol. 2020...Diagnostic Toolkit Enzymes. (unpublished) Taq polymerase and MMLV reverse transcriptase expression plasmids...

  16. Plant Plasmids and Resources

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    ...plant expression vectors containing several cassettes and generate multi-construct transgenic plants -...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use...includes plasmids containing promoters that drive general and species-specific gene expression (see the column...CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the Plant ...selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner...MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes...publications are created. Papers from the plant biology literature are discussed with one of the authors. The podcast...

  17. Fluorescent Protein Guide: Empty Backbones

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    ...Expression mEmerald 487 509 39 6 Monomer (A206K) mEmerald-N1 - Mammalian Expression mEmerald-C1 - Mammalian...Expression mPA-Emerald 487 509 - Monomer (A206K) mPA-Emerald-N1 - Mammalian Expression mPA-Emerald-C1 - Mammalian...HisD-rsTagRFP - Bacterial Expression rsEmerald 491 508 rsEmerald-pBAD - Bacterial Expression rsGFP1 493...Expression Species . The content for this page was generated with the help of Erik Snapp . Empty Backbones ...Mammalian Expression mEmerald-pBAD - Bacterial Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-...

  18. Genomic Deletions in Mammalian Cell Lines

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    ...full-text of the publication here . Article Citation Generation of Genomic Deletions in Mammalian Cell Lines ... incubation time allows for sufficient cell proliferation for screening genomic DNA (gDNA) for the intended... and 7.1 ). The bulk cells have sufficiently proliferated when the concentration exceeds ~100,000 cells...80% confluence. The clones have sufficiently proliferated once macroscopically visible with ~2 mm diameter...pellets in 50 μl of DNA extraction solution. NOTE: Generally ~100,000 cells are used for DNA extraction, although... band” in separate reactions. NOTE: Numerous polymerases may be used for step 6.3 . Run samples in a thermocycler...biallelic deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning...

  19. TALEN Plasmids and Kits

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    ...accessory plasmids available from Addgene for generating your own specific TALE plasmids.... TALENs assembled by Golden Gate. Validated in several species. Takashi Yamamoto Additional Plasmids for... Gate TALEN kit (Bogdanove/Voytas) in order to generate TALORs (TAL Orthongal Repressors) that can be ...Grunwald pCS2TAL3-DD and pCS2TALE3-RR are next generation TALEN backbone vectors designed for use with ...segments (136AA and 63AA, respectfully). This next generation architecture has been shown to increase mutation... C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN(Sangamo)-Destination.... In addition, a T7 promoter was included for generating TALEN mRNAs through in vitro transcription as...

  20. mTOR Pathway

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    ... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer promotes the conditions...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...specific relationship. The content for this page was generated with the help of David Sabatini . mTOR Pathway...specific relationship. The content for this page was generated with the help of David Sabatini . Return to top...
Showing: 61 - 80 of 1000 results