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We narrowed to 935 results for: Ina

Showing: 61 - 80 of 935 results

  1. p53 Pathway

    Type
    Collection
    ...CDK6 Cyclin-dependent kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger ...factor 1 ATM ATM serine/threonine kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed 1...PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific...to activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization...

  2. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...BbsI yes, cut, N-terminal S. pyogenes Zhang PX854 62886 Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang...BbsI yes, cut, N-terminal S. pyogenes Zhang PX852 62884 Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang...activate, N-terminal S. pyogenes Zhang PX856 62888 Mammalian BbsI yes, activate, C-terminal S. pyogenes...assembled into a single T-DNA binary vector of choice through Gateway recombination. Qi Multiplex Conditional...pyogenes GFP Vakoc U6>sgRNA(F+E) 59986 Other/Ciona intestinalis BsaI none S. pyogenes Christiaen p(U6a-BsaI-...Gersbach MuLE System Mammalian MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses...expression cassettes, which can be assembled into the binary CRISPR/Cas9 vectors in one round of cloning by ...

  3. Bacterial Expression Systems

    Type
    Collection
    ... to enhance protein solubility and to direct recombinant proteins to the periplasmic space between the... to the periplasm reduces bacterial protein contamination and facilitates extraction. Browse our most ...NusA Cleavage sites: TEV protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization...gram-negative bacteria with different purification tag combinations, including PelB tags for localization to the...) Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 78577 pL99 PnitA-NitR ε-caprolactam Escherichia...Hypoxia Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 188974 pREDusk-AmpR-MCS FixK2 Red light... Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence (GFP:mRFP1 ratio) Escherichia...

  4. Antibody Production

    Type
    Collection
    ...methods and quality control processes for purified recombinant antibodies....webpage for further information. Production For recombinant antibodies (rAb) encoded by mammalian expression... buffer exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline ...sample is adjusted by diluting or concentrating to a final concentration of 0.9–1.1 mg/mL. Antibody concentration...software and a standard curve is generated. The final antibody concentration is calculated from the standard...performed in parallel with a previous lot of recombinant antibody or the hybridoma-derived equivalent ... Residual Plasmid Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified...

  5. Rett Syndrome

    Type
    Collection
    ...) N-terminal Anti-MECP2 (M7443) mouse antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2...syndrome can be inherited. In females, random X inactivation results in approximately half of cells expressing...expressing mutant MECP2 . Skewing of these X inactivation ratios can affect disease severity. The MECP2...several functional domains, including: the N - t erminal D omain (NTD) the M ethyl B inding D omain (MBD...signal (not essential for localization) the C - t erminal D omain (CTD) The most common missense mutations...antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling...direction and degree of skewing of X-chromosome inactivation. J Med Genet . 44, 148–152. (Link opens in a...

  6. AAV Molecular Tools

    Type
    Collection
    ...of the tet-off transactivator (tTA). 2 Viviana Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter... loop for amplified tTA expression. 1 Viviana Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible... loop for amplified tTA expression. 1 Viviana Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible..., Tet-inducible expression of EYFP. 1 Viviana Gradinaru Tools for Affinity Purification These AAV encode...tdTomato and synaptophysin-EGFP for labeling of axon terminals. 1 Hongkui Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby... and synaptophysin-mRuby for labeling of axon terminals. 1 Liqun Luo 60658 pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato... of neurons through a process reliant on Flp recombinase. 5 Kazunari Miyamichi Genome Engineering Tools...

  7. Plasmids for Stem Cell Research

    Type
    Collection
    ...and shRNA against p53 in different gene/insert combinations A more efficient method to generate integration-free...NR5A2, and microRNA 302/367 in three different combinations of reprogramming factors Efficient germ-line...40. Hochedlinger MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS... and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming to pluripotency...beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells induced Beta Cells Adenoviral Mouse Long-...pancreatic beta cells generated by lineage conversion of acinar cells. Nature Biotechnology. 2014 Dec;32(12):1223... of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...al., 2010; Heinz et al., 2011), engineered to eliminate binding of endogenous mammalian transcription ... by codon optimization of their sequence and eliminating interactions with endogenous factors. Experimental...2017 ) for other viral tTA plasmids. tTA Viviana Gradinaru 104102 pCAG-tTA Mammalian expression of tTA from...AAV vector for dox-inducible expression of Cre recombinase. Takuji Iwasato 63704 pRetroX GFP T2A Cre Retroviral... for dox-inducible expression of GFP T2A Cre recombinase. Floris Foijer 165457 AAVS1-Neo-TRE-CMV-Cre-rtTA...-rtTA Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible...Dox-inducible expression of a light-activated Cre recombinase. Jacco van Rheenen 104102–104112 TetBow Series of plasmids...

  9. Neurodegeneration Research Collection

    Type
    Collection
    ... upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads ...familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic mutations...Huntington’s disease (HD) is a fatal autosomal dominant genetic disorder that causes the progressive degeneration... gene and late-onset AD. It is more likely a combination of gene variation and lifestyle or environmental...exocytosis with VAMP2/3 plasmids to study CNS myelination. (Lam et al. Nat Commun. 2022) Endogenously tag...Innovation Antibody Collection . Explore the recombinant monoclonal antibodies and scFvs for neuroscience... tools and emphasizes their rapid and broad dissemination to the research community. This collection highlights...

  10. CRISPR Plasmids - Tagging

    Type
    Collection
    ...or C-terminal tagging in Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging...deposited a plasmid which introduces an N- or C- terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes...untagged SpCas9 (Addgene 41815 or #44719 ) in combination with gRNA_AAVS1-T2 (Addgene #41818) or using ...modification. This system can be used to create C- and N-terminal epitope tags. The plasmids in the following articles...selection markers are available. An improved FLP recombinase expression vector allows for efficient marker...

  11. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...developing mouse retina. To aide in their studies of the regulatory networks involved in retinal development...plasmids to study the regulatory networks involved in retinal development. Depositor...990 KB) Plasmid and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2...Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or ...Simultaneous deletion of Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification...

  12. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors...creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created... of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved TadA ...modifying the linker region between Cas variant and deaminase to adjust the editing window, or adding fusions... Cas9 is fused to activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing...

  13. Control AAV Preps

    Type
    Collection
    ....eB Viviana Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Viviana Gradinaru 116869 pAAV-...Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Viviana Gradinaru 100896 pAAV.GFA104.PI.eGFP.WPRE.bGH GFA104 EGFP...Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Viviana Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet ...pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Viviana Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre...pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen Flp dependent 1 Viviana Gradinaru 137131 pAAV-Ef1a-Coff/Fon-BFP EF1a BFP Flp dependent...

  14. MAPK Plasmids

    Type
    Collection
    ...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...Browse mitogen-activated protein kinase (MAPK) plasmids. Plasmid...

  15. Plant Plasmids and Resources

    Type
    Collection
    ...innovation for improvement of sustainable agriculture and conservation. Medicinal Plant Genomics Resource (...other monocots - Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for non-transgenic genome...in a new window) - The initiative promotes interdisciplinary exchange, open technologies, and responsible...Transcriptomic and metabolomic resources for 14 key medicinal plants for the advancement of drug production ...

  16. Genetic Code Expansion

    Type
    Collection
    .... Pairs from methanogens like Methanosarcina barkeri and Methanosarcina mazei are orthogonal both in bacteria...Mb-Fluoro-Phe B5 Pyl Fluoro-Phe B5 M. barkeri fluorinated phenylalanine derivatives Mammalian TAG Ryan ...Mb-Fluoro-Phe D6 Pyl Fluoro-Phe D6 M. barkeri fluorinated phenylalanine derivatives Mammalian TAG Ryan ...sites changes to UAG, RF1 function removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church...codon, and tyrU gene replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses...tyrTV and tyrU genes replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...factors and the sensor behavior may differ from the original characterization (e.g. in vitro vs. in vivo, cytoplasmic...Zhou Calcium Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids...Verkhusha Calcium Small near-infrared indicator iGECInano Design and Initial Characterization of a Small...biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7....charges in live cells. Nat Biotechnol. 2017 Mar 13. Katharina Gaus Endo/Exocytosis Superecliptic pHluorin fused...-pHluorin Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal ...expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy...

  18. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression mCardinal 604 659 17 5.3 27 min Prone to dimerization mCardinal-N1 - Mammalian Expression...Expression mCardinal-C1 - Mammalian Expression mCardinal-pBAD - Bacterial Expression mMaroon1 609 657 8.8...fluorescent protein. The content for this page was originally compiled with the help of Erik Snapp . Note for...Chloralkane (CA) pMH-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry...

  19. NETRF

    Type
    Collection
    ... Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors Ramesh Shivdasani Dana-...Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou...Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley ...Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  20. Adenovirus Plasmids

    Type
    Collection
    ... large-scale production. In order to produce recombinant adenoviral vectors, researchers generally need...interest, the ITRs, and homology regions for recombination with the adenoviral plasmid. An adenoviral plasmid...for viral production and homology regions for recombination with the shuttle plasmid. This collection provides...Description PI 16398 BJ5183 E. coli strain for recombination between shuttle plasmid and adenoviral plasmid...
Showing: 61 - 80 of 935 results