We narrowed to 163 results for: NAP
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TypeBlog Post...Finally, using synaptic receptor blockers to distinguish real and artifactual postsynaptic currents (PSCs...
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Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...expression of cytoplasmic-tdTomato and Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen... -
Neurodegeneration Plasmid Collection
TypeCollection...Claudio Alarcon 200789 CLIPf-KIF5A-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...ALS Alison Twelvetrees 200791 KIF5A-SNAPf-HisTag-FLAG KIF5A SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...200792 CLIPf-KIF5A(delta954-1027)-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...CLIPf-KIF5A(1-914; delta505-610)-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...Twelvetrees 200798 CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...MBP, TEV, SNAP PH ALS Anthony Hyman 221886 TH0994-pOCC177-Fus-wt_(1-211) FUS His, MBP, TEV, SNAP PH ALS ... TEV, SNAP PH ALS Anthony Hyman 221888 TH1149-pOCC177-FUS(Nhe)-RGG_RtoK FUS His, MBP, TEV, SNAP PH ALS... -
Plasmids 101: Degron Tags
TypeBlog Post...the presence of a specific protease inhibitor, asunaprevir, the viral protease can no longer self-cleave...components are 1) a SMASh-tagged protein and 2) asunaprevir (small molecule). dTAG The degradation tag (dTAG... -
Experimenting with New Careers while in Grad School
TypeBlog Post... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...a sequence manager A sequence manager, such as SnapGene, can be very helpful to confirm primer design....currently investigating the molecular biology of synaptic transmission and plasticity. Javier García-Nafría... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...expertise to our mutual benefit. Our partnership with SnapGene, for example, allows us to display high-quality... into it. At the same time, this project gives Snapgene great exposure to the community of biological ... -
Hot Plasmids - November 2023
TypeBlog Post...is a scaffolding protein known for its roles in synapse development, transmission, and plasticity and is... is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD-95 [K28/43R] has been... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...primer melting temperature greater than 48℃. Both Snapgene and NEB have tools that help you design primers...video gives a helpful demonstration of how to use Snapgene’s program to design primers for Gibson Assembly... -
Important Considerations When Using AAVs
TypeBlog Post... neurons (retrograde transport) or towards the synapse (anterograde transport), which is a possible mechanism... storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen or a dry ice/ethanol bath... -
Penn Vector Core Partnership with Addgene
TypeCollection...Optogenetics Karl Deisseroth AV-1-26967M 100049-AAV1 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Optogenetics Karl...Optogenetics Karl Deisseroth AV-9-26967M 100049-AAV9 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Optogenetics Karl...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...CI.eGFP.WPRE.rBG James M. Wilson AV-2-26967M 100049-AAV2 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Karl Deisseroth...floxed-eNpHR-EYFP-WPRE-pA Karl Deisseroth AV-5-26967M 100049-AAV5 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Karl Deisseroth... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9 fusions. Here’s some others. SoxS based...including transcription factors, phage proteins, and RNAP components (Dong et al., 2018). They found a transcription... -
Optogenetics AAV Preps
TypeCollection...Bidirectional BiPOLES Promoter CAG CaMKII CBA Dlx EF1a/nEF Synapsin E2 regulatory element Fluorophore GFP Red-wavelength...EYFP Cre dependent PHPeB Gradinaru 107708 pAAV-hSynapsin- soCoChR-GFP Syn CoChR (soma-targeted) GFP Constitutive...Constitutive 9 Boyden 107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn CoChR (soma-targeted) GFP Cre dependent...ChrimsonR tdTomato Flp dependent 8 Boyden 100049 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Syn ChETA EYFP ... -
Adeno-associated virus (AAV) Guide
TypeGuide...developed for their use with rabies virus (RABV) in monosynaptic retrograde tracing of neurons. In this system...express a viral element necessary for RABV trans-synaptic transport. Learn more about these and other initiatives...for neuronal tracing . Browse AAV vectors for monosynaptic neuronal tracing available at Addgene. Optogenetics... from the main cell body, or soma, towards the synapse at the axons. Blood-brain barrier (BBB) Layer of... of molecules, vesicles or organelles from the synapse at the axons towards the main cell body, or soma... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Erik Snapp 49150 BFP-KDEL...GalT signal anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N-10 Golgi apparatus MannII... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog Post...wintertime festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the... -
'Tis the Season to #DeckTheLab
TypeBlog Post... Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your labmates celebrating the... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post... Getting a Faculty Position Author: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware... -
Call for Guest Bloggers
TypeBlog Post...Generating Mouse Models Using CRISPR/Cas9 Erik L. Snapp, Associate Professor at Albert Einstein College ... -
New Viral Vectors - Summer 2024
TypeBlog Post... its bistable nature, it enables inhibition of synapses distant to the site of injection with high temporal...