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We narrowed to 172 results for: NAP

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Showing: 61 - 80 of 172 results

  1. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...a sequence manager A sequence manager, such as SnapGene, can be very helpful to confirm primer design....currently investigating the molecular biology of synaptic transmission and plasticity.     Javier García-Nafría...

  2. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...expertise to our mutual benefit.  Our partnership with SnapGene, for example, allows us to display high-quality... into it. At the same time, this project gives Snapgene great exposure to the community of biological ...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    ...is a scaffolding protein known for its roles in synapse development, transmission, and plasticity and is... is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD-95 [K28/43R] has been...

  5. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...low-throughput, semi-quantitative, and provide only snapshots rather than continuous views of protein activity... attempts to purify or overexpress proteins inappropriate in discerning their true roles. Work conducted...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    ... neurons (retrograde transport) or towards the synapse (anterograde transport), which is a possible mechanism... storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen or a dry ice/ethanol bath...

  8. Brain Initiative Collection

    Type
    Collection
    ...optogenetics. Human Synapsin (hSynapsin) promoter Edward Boyden 107712-AAV9 pAAV-hSynapsin-FLEX-soCoChR-GFP...-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA... viral vector Hillel Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which...Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1...Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter Lin Tian 111068-AAV5 pAAV-hSyn-dLight1.2...Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter Lin Tian 111069-AAV1 pAAV-CAG-dLight1.2...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human...101129 pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control...Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag Control...Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression...expression under T7 control 101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient...transient mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box)... localization Supplemental Information SNAP and CLIP tags SNAP and CLIP tag FAQs (PDF, 252 KB) ACP and...

  10. AAV Molecular Tools

    Type
    Collection
    ... constitutive Expression of pre-synaptic mGRASP-mCerulean for synaptic connectivity mapping. 5 Jinhyun... constitutive Expression of post-synaptic mGRASP-dTomato for synaptic connectivity mapping. 5 Jinhyun ...in the anterograde direction across synapses. Also see Monosynaptic Neuronal Tracers available at Addgene...constitutive Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, ...Cre-dependent Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that...Viviana Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator...pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of the...

  11. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-hSynapsin1-axon-GCaMP6s Syn axon-GCaMP6s none Constitutive 1 Lin Tian 112000 pAAV-hSynapsin1-axon-GCaMP6f...LiLac Promoter CAG CaMKII Dlx EF1a GFAP/GfaABC1D Synapsin Other Activity Cre-dependent Flp-dependent Cre...Serotype PI Acetylcholine Sensors 137950 pAAV.hSynap.iAChSnFR Syn iAChSnFR none Constitutive 1 Loren Looger...Constitutive 1, 9, rg* Marianne Fyhn 172921 pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 Syn axon-jGCaMP8s mRuby3...mRuby3 Constitutive 1, 9 GENIE Project 172923 pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 Syn axon-jGCaMP8f mRuby3...none Constitutive 8 Marianne Fyhn 172922 pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 Syn axon-jGCaMP8m mRuby3... 5, 9 Douglas Kim , GENIE Project 111261 pAAV-hSynapsin1-axon-GCaMP6m Syn axon-GCaMP6m none Constitutive...

  12. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...channel Mouse Mouse IgG2a 140071 Anti-Snapin/SNAPAP [L8/15R] Snapin/SNAPAP Human Mouse IgG2a 140072 Anti-Mitofusin... 177471 Anti-Synapsin-3 [L125/121R] Synapsin-3 Human Mouse IgG2a 177472 Anti-Pan-Synapsin [L125/129R] ... Human Mouse IgG2a 114476 Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a 114477 Anti-TRPML3... Human Mouse IgG2a 114482 Anti-Synaptotagmin-12 [N277/7R] Synaptotagmin-12 Mouse Mouse IgG2a 114483 Anti-GluA1...) Rat Mouse IgG2a 114558 Anti-Synaptotagmin-10 [N269/73R] Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin... Mouse Mouse IgG2a 177526 Anti-Synaptotagmin-7 [N275/14R] Synaptotagmin-7 Mouse Mouse IgG2a 177527 Anti-Lgi1... Human Mouse IgG2a 188216 Anti-Synaptotagmin-6 [N270/47R] Synaptotagmin-6 Mouse Mouse IgG2a 188217 Anti-CASK...

  13. Genetic Code Expansion

    Type
    Collection
    ...Schultz 48696 pANAP AnapRS E. coli 3-(6-acetylnaphthalen-2-ylamino)-2-amino-propanoic acid (Anap) Mammalian...4xEcoLeuT(CUA)_AnapRS AnapRS E. coli 3-(6-acetylnaphthalen-2-ylamino)-2-amino-propanoic acid (Anap) Mammalian...241290 pANAP-P2A-eRF(E55D) AnapRS 3-(6-acetylnaphthalen-2-ylamino)-2-amino-propanoic acid (Anap) Mammalian... is deleted); T7RNAP cassette genomically inserted Michael Jewett 182777 C321.∆A T7RNAP ompT- rne- Recoded...Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF) pyrrolysine (Pyl...TAG Ivana Nikić-Spiegel 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (...Bacterial TAG Thomas Huber 223508 pCMV_NESPylRS(AF)_hU6tRNAPyl PylRS (Y306A, Y384F) M. mazei Mammalian TAG Edward...

  14. AAV for Neuronal Tracing

    Type
    Collection
    ...Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools for Monosynaptic Neuronal...Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with modified...not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype...Wickersham Introduction to Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect... within infected cells, but is required for transynnaptic spread (Wickersham et al., 2007a, Wickersham...cannot spread to any neighboring cells. To enable monosynaptic spread of this deletion-mutant rabies, the essential...

  15. Institute for Protein Innovation

    Type
    Collection
    ...receptor–ligand pairs bridge the presynaptic and postsynaptic membranes at neuronal synapses. Image credit: IPI Browse...Recommended Applications PI Return to top Synaptic Cleft IPI’s Synaptic Cleft Antibody Collection features highly...mediators of synaptic adhesion and function. Additional antibodies targeting other synaptic cleft proteins...Antibody Collection Axon Guidance Antibodies NEW Synaptic Cleft Antibodies NEW Integrin Antibodies Epitope...ligands (Glypicans and Integrins), components in the synapse (Neurexins and Neuroligins), and common epitope...protein families that function at or across the synaptic cleft. Developed using yeast display technology...powerful tools to investigate neurotransmission, synapse formation, neural connectivity, plasticity and ...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...membrane Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal...hippocampal synapses. Neuron. 2006 Sep 21. 51(6):773-86. Leon Lagnado Endo/Exocytosis Synaptophysin with tandem...2023.07.18.549527 Eric Schreiter Calcium GCEPIA1-SNAP ER for sensing ER calcium A genetically encoded ...May 29;7. pii: 32638. Kai Johnsson NADPH/NADP+ NAPstar biosensors for NADPH/NADP+ in plants A family of...sensitivity and scale (4s) or speed (4f) of imaging synaptic transmission Glutamate indicators with increased...glutamate sensors (iGluSnFR3) for improved imaging of synaptic transmission Glutamate indicators with improved...activation kinetics and localization for imaging synaptic transmission. Nat Methods. 2023 May 4. Kaspar ...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Claudio Alarcon 200789 CLIPf-KIF5A-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...ALS Alison Twelvetrees 200791 KIF5A-SNAPf-HisTag-FLAG KIF5A SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...200792 CLIPf-KIF5A(delta954-1027)-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...CLIPf-KIF5A(1-914; delta505-610)-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...Twelvetrees 200798 CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG KIF5A CLIPf, SNAPf, His, Flag CMV, T7 ALS Alison Twelvetrees...MBP, TEV, SNAP PH ALS Anthony Hyman 221886 TH0994-pOCC177-Fus-wt_(1-211) FUS His, MBP, TEV, SNAP PH ALS ... TEV, SNAP PH ALS Anthony Hyman 221888 TH1149-pOCC177-FUS(Nhe)-RGG_RtoK FUS His, MBP, TEV, SNAP PH ALS...

  18. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pENTR4-SNAPf (w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression pSNAP-tag... Expression SNAP-Tag Benzylguanine (BG) pSNAP-tag (m) Vector - Mammalian Expression pSNAPf-C1 - Mammalian...Contributing Authors Written and reviewed by Erik Snapp (Albert Einstein College of Medicine) and the Scientific...

  19. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Optogenetics Karl Deisseroth AV-1-26967M 100049-AAV1 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Optogenetics Karl...Optogenetics Karl Deisseroth AV-9-26967M 100049-AAV9 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Optogenetics Karl...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...CI.eGFP.WPRE.rBG James M. Wilson AV-2-26967M 100049-AAV2 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Karl Deisseroth...floxed-eNpHR-EYFP-WPRE-pA Karl Deisseroth AV-5-26967M 100049-AAV5 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Karl Deisseroth...
Showing: 61 - 80 of 172 results